LOCUS       sequence-229271-         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Flag-HA-6xHIS-Raf1 RBD fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-FHH-Raf1RBD
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     12..36
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             107..130
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             137..163
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             173..190
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt
       61 tttgacctcc atagaagaca ccgactctac tagaggatct accatggatt ataaagatga
      121 cgacgataaa gcaggttacc catacgatgt gcctgactat gctgcaggtt cacatcatca
      181 ccaccatcat ggatccgagc acatacaggg agcttggaag acgatcagca atggttttgg
      241 attcaaagat gccgtgtttg atggctccag ctgcatctct cctacaatag ttcagcagtt
      301 tggctatcag cgccgggcat cagatgatgg caaactcaca gatccttcta agacaagcaa
      361 cactatccgt gttttcttgc cgaacaagca aagaacagtg gtcaatgtgc gaaatggaat
      421 gagcttgcat gactgcctta tgaaagcact caaggtgagg ggcctgcaac cagagtgctg
      481 tgcagtgttc agacttctcc acgaacacaa aggtaaaaaa gcacgcttag attggaatac
      541 tgatgctgcg tctttgattg gagaagaact tcaagtagat ttcctggatc atgttcccct
      601 cacaacacac aactttgctc ggaagacgtt cctgaagctt taactcgagt ctagagggcc
      661 cgacgcgtct ggccaccggt cgcccctctc cctccccccc ccctaacgtt actggccgaa
      721 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
      781 cttttggcaa tgtgagggcc cggaaacct
//
