LOCUS       sequence-229272-         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Flag-HA-6xHIS-Raf1 RBD fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-FHH-Raf1RBD
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        10..61
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     12..32
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     58..82
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             153..176
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             183..209
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             219..236
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
       61 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
      121 aagacaccga ctctactaga ggatctacca tggattataa agatgacgac gataaagcag
      181 gttacccata cgatgtgcct gactatgctg caggttcaca tcatcaccac catcatggat
      241 ccgagcacat acagggagct tggaagacga tcagcaatgg ttttggattc aaagatgccg
      301 tgtttgatgg ctccagctgc atctctccta caatagttca gcagtttggc tatcagcgcc
      361 gggcatcaga tgatggcaaa ctcacagatc cttctaagac aagcaacact atccgtgttt
      421 tcttgccgaa caagcaaaga acagtggtca atgtgcgaaa tggaatgagc ttgcatgact
      481 gccttatgaa agcactcaag gtgaggggcc tgcaaccaga gtgctgtgca gtgttcagac
      541 ttctccacga acacaaaggt aaaaaagcac gcttagattg gaatactgat gctgcgtctt
      601 tgattggaga agaacttcaa gtagatttcc tggatcatgt tcccctcaca acacacaact
      661 ttgctcggaa gacgttcctg aagctttaac tcgagtctag agggcccgac gcgtctggcc
      721 accggtcgcc c
//
