LOCUS       sequence-229274-         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Flag-HA-6xHIS-Mtor Kinase Domain fusion protein in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-FHH-MTORKinaseDomain
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     20..44
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             115..138
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             145..171
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             181..198
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             367..645
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISK"
     CDS             379..651
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQL"
ORIGIN
        1 gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc
       61 cacgctgttt tgacctccat agaagacacc gactctacta gaggatctac catggattat
      121 aaagatgacg acgataaagc aggttaccca tacgatgtgc ctgactatgc tgcaggttca
      181 catcatcacc accatcatgg atcctaccac ccccaggccc tcatctaccc actgacagtg
      241 gcttctaagt ctaccacgac agcccggcac aatgcagcca acaagattct gaagaacatg
      301 tgtgagcaca gcaacaccct ggtccagcag gccatgatgg tgagcgagga gctgatccga
      361 gtggccatcc tctggcatga gatgtggcat gaaggcctgg aagaggcatc tcgtttgtac
      421 tttggggaaa ggaacgtgaa aggcatgttt gaggtgctgg agcccttgca tgctatgatg
      481 gaacggggcc cccagactct gaaggaaaca tcctttaatc aggcctatgg tcgagattta
      541 atggaggccc aagagtggtg caggaagtac atgaaatcag ggaatgtcaa ggacctcacc
      601 caagcctggg acctctatta tcatgtgttc cgacgaatct caaagcagct gcctcagctc
      661 acatccttag agctgcaata tgtttcccca aaacttctga tgtgccggga ccttgaattg
      721 gctgtgccag gaacatatga ccccaaccag ccaatcattc gcattcagtc catagcaccg
      781 tctttgcaag tcatcacatc caagcaga
//
