LOCUS       sequence-229276-       12240 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express VE-cadherin tagged with tdTomato on C-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12240)
  TITLE     Cooper Lab plasmids
REFERENCE   2  (bases 1 to 12240)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBOB-VE-cadherin-tdTomato
FEATURES             Location/Qualifiers
     source          1..12240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        95..398
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        399..602
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     599..623
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             complement(2722..2733)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      3040..3049
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3046..4476
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     misc_feature    4612..5200
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4665..4685)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5083..5094)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5607..5787
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5813..5830)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5819..6043
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6089..6517
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6176..6195)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6386..6407
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6526..6546)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6531..6860
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6711..6846
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6773..6792
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6908..6955
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6974..7348
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7478..7599
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7515..7534)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7569..7588
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7648..7664)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7648..7664)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7661..7683)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7672..7688
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7696..7702,7703..7720,7721..7726))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7741..7762
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7879..7896)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8050..8638)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8130..8149)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8809..9600,9601..9669))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9432..9451
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9670..9774)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9849..9868)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10040..10419
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10421..10619
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             10637..10817
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10864..10989
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11482..11715
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11900..11944
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    join(12196..12240,1..72)
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
       61 attcaaaatt ttatcgataa gcttgggagt tccgcgttac ataacttacg gtaaatggcc
      121 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      181 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      241 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      301 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      361 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      421 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      481 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
      541 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
      601 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
      661 gaagacaccg actctagaat gcagaggctc atgatgctcc tcgccacatc gggcgcctgc
      721 ctgggcctgc tggcagtggc agcagtggca gcagcaggtg ctaaccctgc ccaacgggac
      781 acccacagcc tgctgcccac ccaccggcgc caaaagagag attggatttg gaaccagatg
      841 cacattgatg aagagaaaaa cacctcactt ccccatcatg taggcaagat caagtcaagc
      901 gtgagtcgca agaatgccaa gtacctgctc aaaggagaat atgtgggcaa ggtcttccgg
      961 gtcgatgcag agacaggaga cgtgttcgcc attgagaggc tggaccggga gaatatctca
     1021 gagtaccacc tcactgctgt cattgtggac aaggacactg gtgaaaacct ggagactcct
     1081 tccagcttca ccatcaaagt tcatgacgtg aacgacaact ggcctgtgtt cacgcatcgg
     1141 ttgttcaatg cgtccgtgcc tgagtcgtcg gctgtgggga cctcagtcat ctctgtgaca
     1201 gcagtggatg cagacgaccc cactgtggga gaccacgcct ctgtcatgta ccaaatcctg
     1261 aaggggaaag agtattttgc catcgataat tctggacgta ttatcacaat aacgaaaagc
     1321 ttggaccgag agaagcaggc caggtatgag atcgtggtgg aagcgcgaga tgcccagggc
     1381 ctccgggggg actcgggcac ggccaccgtg ctggtcactc tgcaagacat caatgacaac
     1441 ttccccttct tcacccagac caagtacaca tttgtcgtgc ctgaagacac ccgtgtgggc
     1501 acctctgtgg gctctctgtt tgttgaggac ccagatgagc cccagaaccg gatgaccaag
     1561 tacagcatct tgcggggcga ctaccaggac gctttcacca ttgagacaaa ccccgcccac
     1621 aacgagggca tcatcaagcc catgaagcct ctggattatg aatacatcca gcaatacagc
     1681 ttcatcgtcg aggccacaga ccccaccatc gacctccgat acatgagccc tcccgcggga
     1741 aacagagccc aggtcattat caacatcaca gatgtggacg agccccccat tttccagcag
     1801 cctttctacc acttccagct gaaggaaaac cagaagaagc ctctgattgg cacagtgctg
     1861 gccatggacc ctgatgcggc taggcatagc attggatact ccatccgcag gaccagtgac
     1921 aagggccagt tcttccgagt cacaaaaaag ggggacattt acaatgagaa agaactggac
     1981 agagaagtct acccctggta taacctgact gtggaggcca aagaactgga ttccactgga
     2041 acccccacag gaaaagaatc cattgtgcaa gtccacattg aagttttgga tgagaatgac
     2101 aatgccccgg agtttgccaa gccctaccag cccaaagtgt gtgagaacgc tgtccatggc
     2161 cagctggtcc tgcagatctc cgcaacagac aaggacataa caccacgaaa cgtgaagttc
     2221 aaattcactt tgaatactga gaacaacttt accctcacgg ataatcacga taacacggcc
     2281 aacatcacag tcaagtatgg gcagtttgac cgggagcata ccaaggtcca cttcctaccc
     2341 gtggtcatct cagacaatgg gatgccaagt cgcacgggca ccagcacgct gaccgtggcc
     2401 gtgtgcaagt gcaacgagca gggcgagttc accttctgcg aggatatggc cgcccaggtg
     2461 ggcgtgagca tccaggcagt ggtagccatc ttactctgca tcctcaccat cacagtgatc
     2521 accctgctca tcttcctgcg gcggcggctc cggaagcagg cccgcgcgca cggcaagagc
     2581 gtgccggaga tccacgagca gctggtcacc tacgacgagg agggcggcgg cgagatggac
     2641 accaccagct acgatgtgtc ggtgctcaac tcggtgcgcc gcggcggggc caagcccccg
     2701 cggcccgcgc tggacgcccg gccttccctc tatgcgcagg tgcagaagcc accgaggcac
     2761 gcgcctgggg cacacggagg gcccggggag atggcagcca tgatcgaggt gaagaaggac
     2821 gaggcggacc acgacggcga cggccccccc tacgacacgc tgcacatcta cggctacgag
     2881 ggctccgagt ccatagccga gtccctcagc tccctgggca ccgactcatc cgactctgac
     2941 gtggattacg acttccttaa cgactgggga cccaggttta agatgctggc tgagctgtac
     3001 ggctcggacc cccgggagga gctgctgtat ggaccggtcg ccaccatggt gagcaagggc
     3061 gaggaggtca tcaaagagtt catgcgcttc aaggtgcgca tggagggctc catgaacggc
     3121 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     3181 aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag
     3241 ttcatgtacg gctccaaggc gtacgtgaag caccccgccg acatccccga ttacaagaag
     3301 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggtctg
     3361 gtgaccgtga cccaggactc ctccctgcag gacggcacgc tgatctacaa ggtgaagatg
     3421 cgcggcacca acttcccccc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     3481 gcctccaccg agcgcctgta cccccgcgac ggcgtgctga agggcgagat ccaccaggcc
     3541 ctgaagctga aggacggcgg ccactacctg gtggagttca agaccatcta catggccaag
     3601 aagcccgtgc aactgcccgg ctactactac gtggacacca agctggacat cacctcccac
     3661 aacgaggact acaccatcgt ggaacagtac gagcgctccg agggccgcca ccacctgttc
     3721 ctggggcatg gcaccggcag caccggcagc ggcagctccg gcaccgcctc ctccgaggac
     3781 aacaacatgg ccgtcatcaa agagttcatg cgcttcaagg tgcgcatgga gggctccatg
     3841 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     3901 accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc
     3961 ccccagttca tgtacggctc caaggcgtac gtgaagcacc ccgccgacat ccccgattac
     4021 aagaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     4081 ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg
     4141 aagatgcgcg gcaccaactt cccccccgac ggccccgtaa tgcagaagaa gaccatgggc
     4201 tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac
     4261 caggccctga agctgaagga cggcggccac tacctggtgg agttcaagac catctacatg
     4321 gccaagaagc ccgtgcaact gcccggctac tactacgtgg acaccaagct ggacatcacc
     4381 tcccacaacg aggactacac catcgtggaa cagtacgagc gctccgaggg ccgccaccac
     4441 ctgttcctgt acggcatgga cgagctgtac aagtagagcg gccgcgtcga gtttaaacct
     4501 ggaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg
     4561 gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcaga caatcaacct
     4621 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     4681 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     4741 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     4801 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     4861 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     4921 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     4981 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     5041 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     5101 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     5161 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattcg agctcggtac
     5221 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     5281 ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct
     5341 accacacaca aggctacttc cctgattggc agaactacac accagggcca gggatcagat
     5401 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag aaggtagaag
     5461 aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat gggatggatg
     5521 acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt catcacatgg
     5581 cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc tgagcctggg
     5641 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     5701 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     5761 tttagtcagt gtggaaaatc tctagcaggg ccgctttaaa cccgctgatc agcctcgact
     5821 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     5881 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     5941 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     6001 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     6061 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     6121 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     6181 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     6241 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     6301 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     6361 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     6421 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     6481 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     6541 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     6601 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     6661 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     6721 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     6781 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     6841 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     6901 agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt
     6961 gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt
     7021 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga
     7081 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca
     7141 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc
     7201 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga
     7261 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt
     7321 gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc
     7381 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     7441 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     7501 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     7561 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     7621 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     7681 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     7741 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     7801 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     7861 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     7921 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     7981 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     8041 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     8101 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     8161 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     8221 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     8281 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     8341 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     8401 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     8461 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     8521 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     8581 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     8641 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     8701 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     8761 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     8821 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     8881 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     8941 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     9001 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     9061 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     9121 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     9181 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     9241 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     9301 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     9361 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     9421 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     9481 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     9541 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     9601 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     9661 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     9721 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     9781 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
     9841 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
     9901 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
     9961 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
    10021 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
    10081 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
    10141 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
    10201 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
    10261 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
    10321 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
    10381 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
    10441 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
    10501 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
    10561 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcagc
    10621 gcgttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
    10681 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
    10741 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
    10801 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
    10861 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
    10921 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
    10981 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
    11041 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
    11101 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
    11161 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
    11221 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
    11281 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaaacggc cgctgatctt
    11341 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
    11401 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
    11461 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
    11521 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
    11581 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
    11641 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
    11701 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
    11761 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
    11821 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
    11881 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
    11941 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
    12001 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
    12061 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
    12121 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
    12181 agtgaacgga tcaactttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
//
