LOCUS       sequence-229277-       10971 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express Septin 2 tagged with tdTomato on C-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10971)
  TITLE     Cooper Lab plasmids
REFERENCE   2  (bases 1 to 10971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBOB-Septin 2 -tdTomato
FEATURES             Location/Qualifiers
     source          1..10971
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    274..390
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        413..716
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        717..920
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     917..941
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2089..2098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2095..3525
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     misc_feature    3661..4249
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3714..3734)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4132..4143)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4656..4836
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4862..4879)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4868..5092
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5138..5566
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5225..5244)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5435..5456
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5575..5595)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5580..5909
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5760..5895
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5822..5841
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5957..6004
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6023..6397
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6527..6648
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6564..6583)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6618..6637
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6697..6713)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6697..6713)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6710..6732)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6721..6737
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6745..6751,6752..6769,6770..6775))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6790..6811
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6928..6945)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7099..7687)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7179..7198)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7858..8649,8650..8718))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8481..8500
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8719..8823)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8898..8917)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9089..9468
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9470..9668
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             9686..9866
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9913..10038
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10531..10764
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             join(10949..10971,1..22)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
       61 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
      121 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
      181 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
      241 agacagatcc attcgattag tgaacggatc aacttttaaa agaaaagggg ggattggggg
      301 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
      361 acaaaaacaa attacaaaat tcaaaatttt atcgataagc ttgggagttc cgcgttacat
      421 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      481 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      541 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      601 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      661 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      721 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      781 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      841 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      901 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac
      961 gctgttttga cctccataga agacaccgac tctagaatgt ctaagcaaca gccaactcag
     1021 tttataaatc cagaaacacc tggctatgtt ggatttgcaa acctccccaa tcaagttcac
     1081 cgaaaatcag tgaaaaaagg ttttgagttc acactgatgg tggtcggtga atcaggtcta
     1141 ggaaaatcga ctctcataaa cagcctattc ctaactgatc tgtacccaga aagagtcata
     1201 cctggagcag cagaaaaaat tgaaagaact gtccagattg aggcttcaac tgttgaaatt
     1261 gaagagcgag gggtcaagct acgcctgaca gtggtagata cccctggcta tggtgacgct
     1321 atcaactgca gagattgttt taagacaatt atctcctata ttgatgagca atttgagagg
     1381 tacctgcatg acgagagcgg cttgaacagg cggcacatca ttgataatag ggtgcattgt
     1441 tgcttttact ttatttcacc ttttggacat ggacttaagc ccttagatgt ggcgtttatg
     1501 aaggcaatac acaacaaggt gaatattgtg cctgtcattg caaaagctga cactctcacc
     1561 ctgaaggaac gggagcggct gaagaaaagg attctggatg aaattgaaga acataacatc
     1621 aaaatctatc acttacctga tgcagaatca gatgaagatg aagattttaa agagcagact
     1681 agacttctca aggctagcat cccattctct gtggttggat ccaatcagtt gattgaagcc
     1741 aaaggaaaga aggtcagagg ccgcctctac ccctggggtg ttgtggaagt ggagaaccca
     1801 gagcacaatg actttctgaa gctgagaacc atgctcatca cccacatgca ggatctccag
     1861 gaggtgaccc aggaccttca ttatgaaaac ttccgttctg agagactcaa gagaggcggc
     1921 aggaaagtgg agaatgagga catgaataaa gaccagatct tgctggaaaa agaagctgag
     1981 ctccgccgca tgcaagagat gattgcaagg atgcaggcgc agatgcagat gcagatgcag
     2041 ggcggggatg gcgatggcgg ggctctcggg caccacgtgg gaccggtcgc caccatggtg
     2101 agcaagggcg aggaggtcat caaagagttc atgcgcttca aggtgcgcat ggagggctcc
     2161 atgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     2221 cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg
     2281 tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc accccgccga catccccgat
     2341 tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     2401 ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg acggcacgct gatctacaag
     2461 gtgaagatgc gcggcaccaa cttccccccc gacggccccg taatgcagaa gaagaccatg
     2521 ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc
     2581 caccaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa gaccatctac
     2641 atggccaaga agcccgtgca actgcccggc tactactacg tggacaccaa gctggacatc
     2701 acctcccaca acgaggacta caccatcgtg gaacagtacg agcgctccga gggccgccac
     2761 cacctgttcc tggggcatgg caccggcagc accggcagcg gcagctccgg caccgcctcc
     2821 tccgaggaca acaacatggc cgtcatcaaa gagttcatgc gcttcaaggt gcgcatggag
     2881 ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     2941 ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
     3001 atcctgtccc cccagttcat gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc
     3061 cccgattaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     3121 gaggacggcg gtctggtgac cgtgacccag gactcctccc tgcaggacgg cacgctgatc
     3181 tacaaggtga agatgcgcgg caccaacttc ccccccgacg gccccgtaat gcagaagaag
     3241 accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc
     3301 gagatccacc aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagacc
     3361 atctacatgg ccaagaagcc cgtgcaactg cccggctact actacgtgga caccaagctg
     3421 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg ctccgagggc
     3481 cgccaccacc tgttcctgta cggcatggac gagctgtaca agtagagcgg ccgcgtcgag
     3541 tttaaacctg gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga
     3601 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcagac
     3661 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     3721 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     3781 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     3841 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     3901 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     3961 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     4021 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     4081 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     4141 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     4201 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattcga
     4261 gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     4321 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc
     4381 tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag
     4441 ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga
     4501 aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg
     4561 ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc
     4621 atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct
     4681 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     4741 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     4801 tcagaccctt ttagtcagtg tggaaaatct ctagcagggc cgctttaaac ccgctgatca
     4861 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     4921 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     4981 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     5041 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     5101 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     5161 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     5221 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     5281 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     5341 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     5401 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     5461 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     5521 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     5581 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     5641 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     5701 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     5761 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     5821 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     5881 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     5941 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
     6001 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc
     6061 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt
     6121 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt
     6181 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga
     6241 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc
     6301 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg
     6361 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc
     6421 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     6481 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     6541 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     6601 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     6661 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     6721 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     6781 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     6841 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     6901 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     6961 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     7021 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     7081 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     7141 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     7201 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     7261 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     7321 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     7381 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     7441 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     7501 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     7561 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     7621 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     7681 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     7741 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     7801 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     7861 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     7921 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     7981 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     8041 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     8101 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     8161 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     8221 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     8281 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     8341 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     8401 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     8461 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     8521 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     8581 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     8641 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     8701 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     8761 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     8821 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc
     8881 ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc
     8941 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
     9001 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
     9061 cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa
     9121 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     9181 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     9241 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     9301 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     9361 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     9421 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     9481 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     9541 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     9601 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     9661 ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
     9721 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     9781 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     9841 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     9901 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     9961 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
    10021 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
    10081 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
    10141 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
    10201 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
    10261 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
    10321 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaaacggcc
    10381 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
    10441 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
    10501 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
    10561 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
    10621 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
    10681 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
    10741 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
    10801 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
    10861 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
    10921 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg a
//
