LOCUS       sequence-229278-       11277 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Nfib and hygromycin resistance gene under control of tetO 
            sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11277)
  AUTHORS   Canals I, Ginisty A, Quist E, Timmerman R, Fritze J, Miskinyte G, 
            Monni E, Hansen MG, Hidalgo I, Bryder D, Bengzon J, Ahlenius H
  TITLE     Rapid and efficient induction of functional astrocytes from human 
            pluripotent stem cells.
  JOURNAL   Nat Methods. 2018 Sep;15(9):693-696. doi: 10.1038/s41592-018-0103-2.
            Epub 2018 Aug 20.
  PUBMED    30127505
REFERENCE   2  (bases 1 to 11277)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-018-0103-2"; journalName: "Nat Methods"; date: 
            "2018-09"; volume: "15"; issue: "9"; pages: "693-696"
COMMENT     Sequence Label: tetO.Nfib.Hygro
FEATURES             Location/Qualifiers
     source          1..11277
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        388..586
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             604..784
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    831..956
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1449..1682
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1867..1911
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2209..2326
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2383..2401,2402..2424,2425..2443,2444..2466,2467..2485,
                     2486..2508,2509..2527,2528..2550,2551..2569,2570..2592,
                     2593..2611,2612..2634,2635..2653)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2686..2724
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2721..2745
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    4090..4657
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4107..4659
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4251..4268)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4478..4497
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4674..5699
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             4674..5699
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    5738..6326
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5791..5811)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6209..6220)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6329..6345)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6330..6346)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6851..7031
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7057..7074)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7063..7287
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7333..7761
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7420..7439)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7630..7651
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7770..7790)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7775..8104
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7955..8090
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8017..8036
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8152..8199
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8218..8592
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8722..8843
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8759..8778)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8813..8832
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8892..8908)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8892..8908)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8905..8927)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8916..8932
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8940..8946,8947..8964,8965..8970))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8985..9006
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9123..9140)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9294..9882)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9374..9393)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10053..10844,10845..10913))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10676..10695
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10914..11018)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11093..11112)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
      601 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      661 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      721 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
      781 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
      841 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
      901 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
      961 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1021 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1081 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1141 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1201 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1261 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     1321 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     1381 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     1441 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     1501 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     1561 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     1621 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     1681 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     1741 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     1801 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     1861 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     1921 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     1981 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     2041 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     2101 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
     2161 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
     2221 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2281 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     2341 ggacagcaga gatccagttt ggttagatct cgagtttacc actccctatc agtgatagag
     2401 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     2461 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     2521 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2581 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     2641 tcagtgatag agaaaagtga aagtcgagct cggtacccgg gtcgaggtag gcgtgtacgg
     2701 tgggaggcct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
     2761 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg cggccccgaa
     2821 ttcatgatgt attctcccat ctgtctcact caggatgaat ttcacccatt tattgaggca
     2881 cttcttcctc acgtccgtgc aattgcctat acttggttca acctgcaggc tcgaaaacgc
     2941 aagtacttta aaaagcatga gaaacgaatg tcgaaggatg aagaaagggc agtcaaagac
     3001 gagctgctca gtgagaagcc cgaaatcaag cagaagtggg catccaggct cctggccaaa
     3061 ctgcgcaaag atatccgcca ggagtaccgg gaggactttg tgcttaccgt gactggcaag
     3121 aagcacccgt gctgtgtctt atccaatcca gaccagaagg gtaagattag gaggatcgac
     3181 tgcctgcgac aggcagacaa agtctggcgt ctggatctag tcatggtgat cctgttcaaa
     3241 ggcatccctt tggagagtac ggatggagag cgactcatga agtccccgca ctgcacaaac
     3301 ccagcacttt gtgttcagcc acaccacatc acagtatcag ttaaggagct tgacttgttt
     3361 ttggcatact acgtgcagga gcaagattct ggacaatcag gaagtccaag ccacagtgat
     3421 cctgccaaga atcctccagg gtacctcgag gacagctttg taaaatccgg agtcttcaat
     3481 gtatcagagc ttgtgagagt atccagaaca cccataaccc agggaactgg agtcaacttc
     3541 ccaatcggag aaattcccag ccaaccatac tatcatgaca tgaactctgg tgtgaacctg
     3601 cagaggtcgc tgtcttctcc accgagcagc aaaagaccca aaactatatc tatagatgaa
     3661 aatatggagc caagtcctac aggagacttt tacccctctc caaattcacc agctgctgga
     3721 agtcgaacat ggcatgaacg agatcaagat atgtcttctc caactacaat gaagaagcct
     3781 gagaagccac tgtttagctc tacatctcca caggattctt ccccaagatt gagcactttc
     3841 ccccagcacc atcatcccgg aatacctgga gtcgcgcaca gtgtcatctc aactcgaact
     3901 ccacctccgc cctcaccgtt gccatttccg acgcaagcta tccttcctcc ggcaccttcc
     3961 agctacttct ctcatccaac aatcagatat cctcctcacc tgaatcctca ggatactctg
     4021 aagaactacg taccttctta tgacccatcc agtcctcaaa cgagccagtc ctggtacctg
     4081 ggctagggat ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg
     4141 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
     4201 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
     4261 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
     4321 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
     4381 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
     4441 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
     4501 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
     4561 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca
     4621 cggggacgtg gttttccttt gaaaaacacg atgataatat ggccacagct agaatgaaaa
     4681 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct
     4741 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
     4801 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
     4861 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat
     4921 ttagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc
     4981 tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg
     5041 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
     5101 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
     5161 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
     5221 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca
     5281 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
     5341 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg
     5401 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc
     5461 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
     5521 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
     5581 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
     5641 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaatagt
     5701 taattaagct agctaattcg atatcaagct tatcgataat caacctctgg attacaaaat
     5761 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     5821 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     5881 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     5941 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     6001 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     6061 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     6121 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     6181 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     6241 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     6301 gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgagacc tagaaaaaca
     6361 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc
     6421 acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac
     6481 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     6541 aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
     6601 cttccctgat tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg
     6661 atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca atgaaggaga
     6721 gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
     6781 attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc
     6841 ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     6901 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     6961 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     7021 aatctctagc agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     7081 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     7141 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     7201 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     7261 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     7321 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     7381 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     7441 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     7501 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     7561 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     7621 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     7681 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     7741 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     7801 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     7861 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     7921 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     7981 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     8041 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     8101 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat
     8161 taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg
     8221 gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag
     8281 ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg
     8341 gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac
     8401 accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc
     8461 gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg
     8521 tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag
     8581 gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg
     8641 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     8701 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     8761 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     8821 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     8881 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     8941 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     9001 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     9061 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     9121 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     9181 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     9241 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     9301 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     9361 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     9421 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     9481 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     9541 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     9601 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     9661 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     9721 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     9781 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     9841 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     9901 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     9961 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    10021 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    10081 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    10141 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    10201 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    10261 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    10321 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    10381 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    10441 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    10501 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    10561 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    10621 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    10681 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    10741 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    10801 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    10861 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    10921 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    10981 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    11041 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
    11101 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
    11161 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
    11221 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatata
//
