LOCUS       sequence-22928-e         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  TITLE     Other Plasmids
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: V50 pCS2+mRFP-N3
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(14..688)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             complement(14..688)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(73..96)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(271..290)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     540..558
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     promoter        complement(723..741)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(724..741)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 cgatgggatc cttggcgccg gtggagtggc ggccctcggc gcgctcgtac tgttccacga
       61 tggtgtagtc ctcgttgtgg gaggtgatgt ccagcttgat gtcggtcttg taggcgccgg
      121 gcagctgcac gggcttcttg gccatgtagg tggtcttgac ctcggcgtcg tagtggccgc
      181 cgtccttcag cttcagcctc atcttgatct cgcccttcag ggcgccgtcc tcggggtaca
      241 tccgctcggt ggaggcctcc cagcccatgg tcttcttctg cattacgggg ccgtcggagg
      301 ggaagttggt gccgcgcagc ttcaccttgt agatgaactc gccgtcctgc agggaggagt
      361 cctgggtcac ggtcacaacg ccgccgtcct cgaagttcat cacgcgctcc cacttgaagc
      421 cctcggggaa ggacagcttc aagtagtcgg ggatgtcggc ggggtgcttc acgtaggcct
      481 tggagccgta ctggaactga ggggacagga tgtcccaggc gaagggcagg gggccgccct
      541 tggtcacctt cagcttggcg gtctgggtgc cctcgtaggg gcggccctcg ccctcgccct
      601 cgatctcgaa ctcgtggccg ttcacggagc cctccatgcg caccttgaag cgcatgaact
      661 ccttgatgac gtcctcggag gaggccatgg tagatcctgc aaaaagaaca agtagcttgt
      721 attctatagt gtcacctaaa tcaagcttgc tccgacgtcc ccaggcagaa tggcggttcc
      781 ctaaacgagc attgcttata tagacctccc attangcacg cctaccgccc atttacgtca
      841 atggaacgcc catttgcgtc attgcccctc cccattgacg tcaatgggga tgtacttggc
      901 agccatcgcg ggccatttac cgccattgac gtcaatggga gtactgccaa tgtaccctgg
      961 cgtacttnc
//
