LOCUS       sequence-229280-        2248 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Promoter J23102 in pStA0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Taylor GM, Mordaka PM, Heap JT
  TITLE     Start-Stop Assembly: a functionally scarless DNA assembly system 
            optimized for metabolic engineering.
  JOURNAL   Nucleic Acids Res. 2018 Nov 20. pii: 5193345. doi: 
            10.1093/nar/gky1182.
  PUBMED    30462270
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Nov 20. pii: 5193345. doi: 10.1093/nar/gky1182."
COMMENT     Sequence Label: pStA0::J23102
FEATURES             Location/Qualifiers
     source          1..2248
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..87)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(100..118)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        186..290
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(291..359,360..1151)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(509..528)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1322..1910
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1811..1830
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      2068..2162
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        2182..2216
                     /label=BBa_J23102
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.86"
ORIGIN
        1 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
       61 tttcgactga gcctttcgtt ttatttgatg cccgaaaggg cctcgtgata cgcctatttt
      121 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
      181 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
      241 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
      301 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
      361 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
      421 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
      481 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
      541 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
      601 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
      661 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
      721 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
      781 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
      841 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
      901 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
      961 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtcc cgcggtatca
     1021 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     1081 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     1141 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     1201 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     1261 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     1321 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     1381 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     1441 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     1501 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     1561 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     1621 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     1681 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     1741 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     1801 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     1861 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     1921 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     1981 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     2041 gccgcagccg aacgaccgag cgccttggac tcctgttgat agatccagta atgacctcag
     2101 aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt attggtgaga
     2161 atccagtgtg gctcttcgca gttgacagct agctcagtcc taggtactgt gctagcccat
     2221 gaagagcgta agacctctag ggcggcgg
//
