LOCUS       sequence-229282-         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Flag-HA-6xHIS-Mtor HEAT Domain fusion protein in mammalian
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-FHH-MTOR HEAT
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     12..36
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             107..130
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             137..163
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             173..190
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt
       61 tttgacctcc atagaagaca ccgactctac tagaggatct accatggatt ataaagatga
      121 cgacgataaa gcaggttacc catacgatgt gcctgactat gctgcaggtt cacatcatca
      181 ccaccatcat ggatccatgc ttggaaccgg acctgccgcc gccaccaccg ctgccaccac
      241 atctagcaat gtgagcgtcc tgcagcagtt tgccagtggc ctaaagagcc ggaatgagga
      301 aaccagggcc aaagccgcca aggagctcca gcactatgtc accatggaac tccgagagat
      361 gagtcaagag gagtctactc gcttctatga ccaactgaac catcacattt ttgaattggt
      421 ttccagctca gatgccaatg agaggaaagg tggcatcttg gccatagcta gcctcatagg
      481 agtggaaggt gggaatgcca cccgaattgg cagatttgcc aactatcttc ggaacctcct
      541 cccctccaat gacccagttg tcatggaaat ggcatccaag gccattggcc gtcttgccat
      601 ggcaggggac acttttaccg ctgagtacgt ggaatttgag gtgaagcgag ccctggaatg
      661 gctgggtgct gaccgcaatg agggccggag acatgcagct gtcctggttc tccgtgagct
      721 ggccatcagc gtccctacct tcttcttcca gcaagtgcaa cccttctttg acaacatttt
      781 tgtggccgtg tgggacccca aacaggccat ccg
//
