LOCUS       sequence-229286-        2983 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  crtW in pStA0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Taylor GM, Mordaka PM, Heap JT
  TITLE     Start-Stop Assembly: a functionally scarless DNA assembly system 
            optimized for metabolic engineering.
  JOURNAL   Nucleic Acids Res. 2018 Nov 20. pii: 5193345. doi: 
            10.1093/nar/gky1182.
  PUBMED    30462270
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Nov 20. pii: 5193345. doi: 10.1093/nar/gky1182."
COMMENT     Sequence Label: pStA0::crtW
FEATURES             Location/Qualifiers
     source          1..2983
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      116..210
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      complement(1032..1118)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1131..1149)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1217..1321
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1322..1390,1391..2182)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1540..1559)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2353..2941
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2842..2861
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
       61 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg ccttggactc
      121 ctgttgatag atccagtaat gacctcagaa ctccatctgg atttgttcag aacgctcggt
      181 tgccgccggg cgttttttat tggtgagaat ccagtgtgct cttcgatggt ccagtgccag
      241 ccgtcgtcgc ttcattctga aaaactcgtt cttctctcga gcactattcg cgatgataaa
      301 aacatcaaca agggcatttt catcgcctgc tttattttgt ttctctgggc catttctctg
      361 attcttctgc tgtccattga tacttcaatt attcataaat ctctgctggg tatcgcaatg
      421 ctttggcaga catttttata taccggcctc ttcattacag cgcacgatgc tatgcatggc
      481 gttgtttatc cgaaaaaccc gcgcattaat aatttcattg gaaaactgac gctgattctg
      541 tatggtctgt taccgtataa agatctgtta aaaaagcatt ggcttcatca tggtcatccg
      601 ggtaccgatc tggatccgga ttactacaat ggccatcccc agaatttttt cttatggtac
      661 ttgcacttta tgaaaagcta ttggcggtgg acccaaattt tcggccttgt gatgattttc
      721 cacggactga aaaatctggt acatattccg gagaataatt tgatcatctt ttggatgatt
      781 ccgtccattt tgtccagtgt gcaactcttt tactttggga cattcttacc gcataaaaaa
      841 ctggaaggcg gctataccaa tccccactgc gctcgctcca ttccgctccc gctgttctgg
      901 agctttgtga catgttatca cttcggctat cataaagaac atcacgaata tcctcaactc
      961 ccgtggtgga aactgcctga agcacacaaa atctcgctgt aatgaagagc gtaagacctc
     1021 tagggcggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
     1081 aaggcccagt ctttcgactg agcctttcgt tttatttgat gcccgaaagg gcctcgtgat
     1141 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     1201 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     1261 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     1321 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     1381 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     1441 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     1501 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     1561 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     1621 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     1681 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     1741 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     1801 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     1861 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     1921 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     1981 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     2041 ccgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     2101 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     2161 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     2221 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     2281 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     2341 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2401 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2461 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     2521 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2581 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2641 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2701 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     2761 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2821 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2881 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     2941 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgc
//
