LOCUS       sequence-229288-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Flag-HA-6xHIS-MAPKAP1 RBD fusion protein in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-FHH-MAPKAP1RBD
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..44
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     primer_bind     41..65
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             136..159
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             166..192
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             202..219
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(660..677)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
       61 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgactctact
      121 agaggatcta ccatggatta taaagatgac gacgataaag caggttaccc atacgatgtg
      181 cctgactatg ctgcaggttc acatcatcac caccatcatg gatccctctt tgttcgaata
      241 aatgctgctc atggattctc ccttattcag gtggacaaca caaaggttac catgaaggaa
      301 atcttactga aggcagtgaa gcgaagaaaa ggatcccaga aagtttcagg ccctcagtac
      361 cgcctggaga agcagagcga gcccaatgtc gccgttgacc tggacagcac tttggagagc
      421 cagagcgcat gggagttctg cctggtccgc taactcgagt ctagagggcc cgacgcgtct
      481 ggccaccggt cgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga
      541 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa
      601 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc
      661 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc
      721 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg
      781 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca
      841 accccagtgc cacgttgtga gttggatagt tgtggaaaga
//
