LOCUS       sequence-229289-         451 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Flag-HA-6xHIS-MAPKAP1 RBD fusion protein in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-FHH-MAPKAP1RBD
FEATURES             Location/Qualifiers
     source          1..451
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             94..117
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             124..150
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             160..177
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
       61 gaagacaccg actctactag aggatctacc atggattata aagatgacga cgataaagca
      121 ggttacccat acgatgtgcc tgactatgct gcaggttcac atcatcacca ccatcatgga
      181 tccctctttg ttcgaataaa tgctgctcat ggattctccc ttattcaggt ggacaacaca
      241 aaggttacca tgaaggaaat cttactgaag gcagtgaagc gaagaaaagg atcccagaaa
      301 gtttcaggcc ctcagtaccg cctggagaag cagagcgagc ccaatgtcgc cgttgacctg
      361 gacagcactt tggagagcca gagcgcatgg gagttctgcc tggtccgcta actcgagtct
      421 agagggcccg acgcgtctgg ccaccggtcg c
//
