LOCUS       sequence-229290-        2734 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  crtZ in pStA0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Taylor GM, Mordaka PM, Heap JT
  TITLE     Start-Stop Assembly: a functionally scarless DNA assembly system 
            optimized for metabolic engineering.
  JOURNAL   Nucleic Acids Res. 2018 Nov 20. pii: 5193345. doi: 
            10.1093/nar/gky1182.
  PUBMED    30462270
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Nov 20. pii: 5193345. doi: 10.1093/nar/gky1182."
COMMENT     Sequence Label: pStA0::crtZ
FEATURES             Location/Qualifiers
     source          1..2734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      102..196
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      complement(769..855)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(868..886)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        954..1058
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1059..1127,1128..1919)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1277..1296)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2090..2678
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2579..2598
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
       61 agctgatacc gctcgccgca gccgaacgac cgagcgcctt ggactcctgt tgatagatcc
      121 agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc gccgggcgtt
      181 ttttattggt gagaatccag tgtgctcttc gatgctgtgg atttggaatg ctctgatcgt
      241 gtttgttacc gtcattggca tggaagttat cgcggctctg gcccacaaat atattatgca
      301 tggttgggga tggggttggc atttgtcgca ccatgagccg cgcaagggag cgtttgaagt
      361 gaacgatctg tatgcagtag tttttgcggc ccttagcatt cttctgattt atctgggctc
      421 gaccggcatg tggcccttgc aatggatcgg tgcgggcatg acggcttacg gcttgcttta
      481 ttttatggtt cacgatggat tagttcacca gcgctggcca ttccgttata tcccacgcaa
      541 aggctattta aaacgtcttt acatggcaca tcgtatgcat catgcggttc gggggaaaga
      601 aggctgcgtg agctttgggt ttctctatgc gccgcctctt tctaaactgc aagctaccct
      661 gcgtgaacgg catggcgcgc gcgcaggcgc ggctcgtgat gcgcagggtg gcgaagatga
      721 acccgcatct ggcaaataat gaagagcgta agacctctag ggcggcggat ttgtcctact
      781 caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc
      841 ctttcgtttt atttgatgcc cgaaagggcc tcgtgatacg cctattttta taggttaatg
      901 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
      961 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     1021 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     1081 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     1141 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     1201 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     1261 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     1321 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     1381 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     1441 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     1501 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     1561 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     1621 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     1681 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     1741 ttattgctga taaatctgga gccggtgagc gtgggtcccg cggtatcatt gcagcactgg
     1801 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     1861 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     1921 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     1981 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     2041 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     2101 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     2161 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     2221 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2281 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     2341 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     2401 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     2461 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     2521 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     2581 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     2641 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     2701 tacggttcct ggccttttgc tggccttttg ctca
//
