LOCUS       sequence-229293-         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Flag-HA-6xHIS-MAPKAP1 RBD Triple Mutant fusion protein in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-FHH-MAPKAP1RBD/Triple Mutant
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     17..41
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             112..135
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             142..168
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             178..195
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(636..653)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac
       61 gctgttttga cctccataga agacaccgac tctactagag gatctaccat ggattataaa
      121 gatgacgacg ataaagcagg ttacccatac gatgtgcctg actatgctgc aggttcacat
      181 catcaccacc atcatggatc cctctttgtt cgaataaatg ctgctcatgg attctccctt
      241 attcaggtgg acaacacaaa ggttaccatg aaggaaatct tactggcggc agtggcgcga
      301 ctaaaaggat cccagaaagt ttcaggccct cagtaccgcc tggagaagca gagcgagccc
      361 aatgtcgccg ttgacctgga cagcactttg gagagccaga gcgcatggga gttctgcctg
      421 gtccgctaac tcgagtctag agggcccgac gcgtctggcc accggtcgcc cctctccctc
      481 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
      541 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
      601 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
      661 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
      721 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggcc
//
