LOCUS       sequence-229294-         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Flag-HA-6xHIS-MAPKAP1 RBD Triple Mutant fusion protein in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-FHH-MAPKAP1RBD/Triple Mutant
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        134..337
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     288..308
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     334..358
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             429..452
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             459..485
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             495..512
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
       61 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      121 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      181 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      241 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      301 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
      361 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactct actagaggat
      421 ctaccatgga ttataaagat gacgacgata aagcaggtta cccatacgat gtgcctgact
      481 atgctgcagg ttcacatcat caccaccatc atggatccct ctttgttcga ataaatgctg
      541 ctcatggatt ctcccttatt caggtggaca acacaaaggt taccatgaag gaaatcttac
      601 tggcggcagt ggcgcgacta aaaggatccc agaaagtttc aggccctcag taccgcctgg
      661 agaagcagag cgagcccaat gtcgccgttg acctggacag cactttggag agccagagcg
      721 catgggagtt ctgcctggtc cgctaactcg agtctagagg gcccgacgcg tctggccacc
      781 ggtcgcccct ct
//
