LOCUS       sequence-229295-        3124 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  idi in pStA0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Taylor GM, Mordaka PM, Heap JT
  TITLE     Start-Stop Assembly: a functionally scarless DNA assembly system 
            optimized for metabolic engineering.
  JOURNAL   Nucleic Acids Res. 2018 Nov 20. pii: 5193345. doi: 
            10.1093/nar/gky1182.
  PUBMED    30462270
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Nov 20. pii: 5193345. doi: 10.1093/nar/gky1182."
COMMENT     Sequence Label: pStA0::idi
FEATURES             Location/Qualifiers
     source          1..3124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     214..233
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      471..565
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      complement(1528..1614)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1627..1645)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1713..1817
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1818..1886,1887..2678)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2036..2055)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(2849..3124,1..313)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
       61 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
      121 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
      181 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
      241 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
      301 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
      361 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
      421 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgccttg gactcctgtt
      481 gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg
      541 ccgggcgttt tttattggtg agaatccagt gtgctcttcg atgctgcgct cgcttctgcg
      601 cggtttaact cacatcccgc gcgtcaactc ggcccaacag ccttcatgcg cacacgcgcg
      661 cttacaattt aagctgcgtt cgatgcagat gactctgatg caaccgagta tctcagctaa
      721 cctgtcgcgc gccgaagatc gtactgacca catgcgcggt gcaagtacgt gggctggtgg
      781 tcaatcccaa gatgaactga tgttaaagga cgaatgcatt ttggttgatg ttgaagataa
      841 cattactgga catgcatcaa agttggaatg tcataaattt ctgccgcacc aacccgcagg
      901 tttgttgcac cgcgccttca gtgtatttct gtttgatgac caaggtcgtc tgttgcttca
      961 acaacgggca cgctcgaaaa ttacctttcc gagtgtatgg accaatactt gttgcagtca
     1021 tccattgcac ggtcagacgc cagatgaagt ggatcaactg agccaggtcg cggacgggac
     1081 cgtcccgggg gctaaggcgg ccgccatccg gaaactggaa catgaactgg gtatcccggc
     1141 ccatcaactg ccagcttcag cgtttcgttt cctcacccgt ttgcattact gtgcggctga
     1201 cgtgcagccg gcggctacgc agtcagcact ttggggggaa catgaaatgg attatatcct
     1261 gttcattcgt gcgaatgtaa cactcgcccc gaatcctgac gaagtggacg aggtccgtta
     1321 tgtaacgcag gaagaactgc gccagatgat gcaaccggat aatggccttc agtggagtcc
     1381 atggttccgt attattgccg cccggtttct tgaacgttgg tgggccgatc tggacgcagc
     1441 actgaacacg gataaacatg aagattgggg taccgtgcat catattaatg aggcataatg
     1501 aagagcgtaa gacctctagg gcggcggatt tgtcctactc aggagagcgt tcaccgacaa
     1561 acaacagata aaacgaaagg cccagtcttt cgactgagcc tttcgtttta tttgatgccc
     1621 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     1681 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     1741 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     1801 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     1861 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     1921 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     1981 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     2041 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     2101 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     2161 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     2221 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     2281 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     2341 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     2401 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     2461 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     2521 ccggtgagcg tgggtcccgc ggtatcattg cagcactggg gccagatggt aagccctccc
     2581 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     2641 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     2701 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     2761 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     2821 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     2881 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     2941 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     3001 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3061 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     3121 tgga
//
