LOCUS       sequence-229297-         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Frb:Akt2 fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-Frb:Akt2-IRES-Hygromycin
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..44
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     primer_bind     41..65
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             133..411
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
ORIGIN
        1 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
       61 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgactctact
      121 agaggatcca tgatcctctg gcatgagatg tggcatgaag gcctggaaga ggcatctcgt
      181 ttgtactttg gggaaaggaa cgtgaaaggc atgtttgagg tgctggagcc cttgcatgct
      241 atgatggaac ggggccccca gactctgaag gaaacatcct ttaatcaggc ctatggtcga
      301 gatttaatgg aggcccaaga gtggtgcagg aagtacatga aatcagggaa tgtcaaggac
      361 ctcctccaag cctgggacct ctattatcat gtgttccgac gaatctcaaa gacgcgtgcc
      421 accatgaatg aggtgtctgt catcaaagaa ggctggctcc acaagcgtgg tgaatacatc
      481 aagacctgga ggccacggta cttcctgctg aagagcgacg gctccttcat tgggtacaag
      541 gagaggcccg aggcccctga tcagactcta ccccccttaa acaacttctc cgtagcagaa
      601 tgccagctga tgaagaccga gaggccgcga cccaacacct ttgtcatacg ctgcctgcag
      661 tggaccacag tcatcgagag gaccttccac gtggattctc cagacgagag ggaggagtgg
      721 atgcgggcca tccagatggt cgccaacagc ctcaagcagc gggccccagg cgaggacccc
      781 atggactaca agtgtggctc ccccagtgac tcctccacga ctgaggagat ggaagtggcg
      841 gtcagcaagg cacgggctaa agtgaccatg aatgacttc
//
