LOCUS       sequence-22930-e         698 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 698)
  TITLE     Other Plasmids
REFERENCE   2  (bases 1 to 698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: M11 Cardiac Actin Promoter  pCarA
FEATURES             Location/Qualifiers
     source          1..698
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(104..123)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(105..123)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(130..147)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(130..146)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(139..161)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      288..668
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(375..394)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..606
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
       61 tgatcctcta gagcggccgc caccgcggtg gagctccaat tcgccctata gtgagtcgta
      121 ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
      181 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
      241 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
      301 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
      361 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
      421 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
      481 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
      541 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
      601 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
      661 gatttcggcc tattggttaa aaaatgagct gatttaac
//
