LOCUS       sequence-229302-         697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses membrane-targeted recruiter protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-FKBP-Kras-Blasticidin
FEATURES             Location/Qualifiers
     source          1..697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     31..55
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(120..122,123..443)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     primer_bind     complement(613..631)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg
       61 gagacgccat ccacgctgtt ttgacctcca tagaagacac cgactctact agaggatcca
      121 tgggagtgca ggtggagact atctccccag gagacgggcg caccttcccc aagcgcggcc
      181 agacctgcgt ggtgcactac accgggatgc ttgaagatgg aaagaaattt gattcctccc
      241 gggacagaaa caagcccttt aagtttatgc taggcaagca ggaggtgatc cgaggctggg
      301 aagaaggggt tgcccagatg agtgtgggtc agagagccaa actgactata tctccagatt
      361 atgcctatgg tgccactggg cacccaggca tcatcccacc acatgccact ctcgtcttcg
      421 atgtggagct tctaaaactg gaaggtgctg cacttctgcc cggttctatg gtgagcaagg
      481 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
      541 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
      601 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
      661 tcctgtcccc tcagttcatg tacggctcca aggccta
//
