LOCUS       sequence-229303-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses membrane-targeted recruiter protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-FKBP-Kras-Blasticidin
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(20..38)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     288..307
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     regulatory      592..601
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             604..630
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc
       61 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc
      121 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
      181 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
      241 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
      301 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
      361 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc
      421 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc
      481 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc
      541 gagggccgcc actccaccgg cggcatggac gagctgtaca agcccggttc tgccgccatg
      601 gcatatccat atgacgtccc agactatgct ggcttagttc gagaaattcg aaaacataaa
      661 gaaaagatga gcaaagatgg taaaaagaag aaaaagaagt caaagacaaa gtgtgtaatt
      721 atgtaactcg agtctagagg gcccgacgcg tctggccacc ggtcgcccct c
//
