LOCUS       sequence-229304-        2248 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Promoter J23118 in pStA0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Taylor GM, Mordaka PM, Heap JT
  TITLE     Start-Stop Assembly: a functionally scarless DNA assembly system 
            optimized for metabolic engineering.
  JOURNAL   Nucleic Acids Res. 2018 Nov 20. pii: 5193345. doi: 
            10.1093/nar/gky1182.
  PUBMED    30462270
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Nov 20. pii: 5193345. doi: 10.1093/nar/gky1182."
COMMENT     Sequence Label: pStA0::J23118
FEATURES             Location/Qualifiers
     source          1..2248
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(13..31)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        99..203
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(204..272,273..1064)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(422..441)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1235..1823
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1724..1743
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      1981..2075
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        2095..2129
                     /label=BBa_J23118
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.56"
     terminator      complement(2162..2248)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 atgcccgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
       61 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
      121 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
      181 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
      241 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
      301 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
      361 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
      421 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
      481 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
      541 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
      601 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
      661 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
      721 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
      781 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
      841 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
      901 ctggagccgg tgagcgtggg tcccgcggta tcattgcagc actggggcca gatggtaagc
      961 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     1021 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     1081 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     1141 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     1201 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     1261 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     1321 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     1381 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     1441 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     1501 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     1561 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     1621 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     1681 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     1741 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     1801 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     1861 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     1921 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgccttg
     1981 gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc
     2041 tcggttgccg ccgggcgttt tttattggtg agaatccagt gtggctcttc gcagttgacg
     2101 gctagctcag tcctaggtat tgtgctagcc catgaagagc gtaagacctc tagggcggcg
     2161 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
     2221 ctttcgactg agcctttcgt tttatttg
//
