LOCUS       sequence-229306-         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses early endosome-targeted recruiter protein in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-FKBP-Rab5-Blasticidin
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..44
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     primer_bind     41..65
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(130..132,133..453)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     primer_bind     complement(623..641)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     891..910
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
       61 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgactctact
      121 agaggatcca tgggagtgca ggtggagact atctccccag gagacgggcg caccttcccc
      181 aagcgcggcc agacctgcgt ggtgcactac accgggatgc ttgaagatgg aaagaaattt
      241 gattcctccc gggacagaaa caagcccttt aagtttatgc taggcaagca ggaggtgatc
      301 cgaggctggg aagaaggggt tgcccagatg agtgtgggtc agagagccaa actgactata
      361 tctccagatt atgcctatgg tgccactggg cacccaggca tcatcccacc acatgccact
      421 ctcgtcttcg atgtggagct tctaaaactg gaaggtgctg cacttctgcc cggttctatg
      481 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg
      541 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
      601 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
      661 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc
      721 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
      781 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
      841 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
      901 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
//
