LOCUS       sequence-229307-         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses early endosome-targeted recruiter protein in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-FKBP-Rab5-Blasticidin
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      82..91
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             94..120
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
       61 gagctgtaca agcccggttc tgccgccatg gcatatccat atgacgtccc agactatgct
      121 ggcgctagtc gaggcgcaac aagacccaac gggccaaata ctggaaataa aatatgccag
      181 ttcaaactag tacttctggg agagtctgct gttggcaaat caagcctagt gcttcgtttt
      241 gtgaagggcc aatttcatga atttcaagag agtaccatag gggctgcttt tctaacccaa
      301 actgtgtgtc ttgatgatac aacagtaaag tttgaaatat gggatacagc tggtcaagaa
      361 cgataccata gcttagcacc aatgtactac agaggagcac aagcagccat agttgtatat
      421 gatatcacaa atgaggagtc ctttgccaga gccaaaaact gggttaaaga acttcagagg
      481 caagccagtc ctaacattgt aatagcttta tcaggaaaca aggctgatct tgcaaataaa
      541 agagctgtcg atttccagga agcacagtcc tatgcagatg acaacagttt attattcatg
      601 gagacatcag ctaaaacatc gatgaatgta aatgaaatat tcatggcaat agctaaaaag
      661 ttgccaaaga acgaaccaca gaatccagga gcaaattctg ccagaggaag aggagtagac
      721 cttactgaac ccacgcagcc aaccaggaat cagtgttgta gtaactaact cgagtctaga
      781 gggcccgacg cgtctggcca ccggtcgccc ctctccctcc ccccc
//
