LOCUS       sequence-229308-        6894 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  human CD155 coding sequence subcloned into pMP71 retroviral plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6894)
  AUTHORS   Josefsson SE, Beiske K, Blaker YN, Forsund MS, Holte H, Ostenstad B,
            Kimby E, Koksal H, Walchli S, Bai B, Smeland EB, Levy R, Kolstad A, 
            Huse K, Myklebust JH
  TITLE     TIGIT and PD-1 Mark Intratumoral T Cells with Reduced Effector 
            Function in B-cell Non-Hodgkin Lymphoma.
  JOURNAL   Cancer Immunol Res. 2019 Mar;7(3):355-362. doi: 
            10.1158/2326-6066.CIR-18-0351. Epub 2019 Jan 18.
  PUBMED    30659053
REFERENCE   2  (bases 1 to 6894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2326-6066.CIR-18-0351"; journalName: "Cancer Immunol Res"; 
            date: "2019-03"; volume: "7"; issue: "3"; pages: "355-362"
COMMENT     Sequence Label: pMP71-hCD155
FEATURES             Location/Qualifiers
     source          1..6894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     668..687
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        668..686
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    718..742
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2037..2061)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2306..2709
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             3215..3805
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(3830..3846)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3830..3846)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3843..3865)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3854..3870
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3878..3884,3885..3902,3903..3908))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3923..3944
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4061..4078)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4232..4820)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(4991..5782,5783..5851))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5614..5633
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5852..5956)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6024..6042
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6080..6102)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6202..6221
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6415..6437
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6429..6446
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6430..6446
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gcgtcgcccg ggtacccgta ttcccaataa agcctcttgc tgtttgcatc cgaatcgtgg
       61 actcgctgat ccttgggagg gtctcctcag attgattgac tgcccacctc gggggtcttt
      121 catttggagg ttccaccgag atttggagac ccctgcccag ggaccaccga cccccccgcc
      181 gggaggtaag ctggccagcg gtcgtttcgt gtctgtctct gtctttgtgc gtgtttgtgc
      241 cggcatctaa tgtttgcgcc tgcgtctgta ctagttggct aactagatct gtatctggcg
      301 gtcccgcgga agaactgacg agttcgtatt cccggccgca gcccctggga gacgtcccag
      361 cggcctcggg ggcccgtttt gtggcccatt ctgtatcagt taacctaccc gagtcggact
      421 ttttggagct ccgccactgt ccgaggggta cgtggctttg ttgggggacg agagacagag
      481 acacttcccg cccccgtctg aatttttgct ttcggtttta cgccgaaacc gcgccgcgcg
      541 tcttgtctgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
      601 tgaaaattag ctcgacaaag ttaagtaata gtccctctct ccaagctcac ttacaggcgg
      661 cctcgagtaa tacgactcac tatagggaga cccaagctgg ctaggtaagc ttgatcaaca
      721 agtttgtaca aaaaagcagg ctccgcggcc gcccccttca ccatggcccg agccatggcc
      781 gccgcgtggc cgctgctgct ggtggcgcta ctggtgctgt cctggccacc cccaggaacc
      841 ggggacgtcg tcgtgcaggc gcccacccag gtgcccggct tcttgggcga ctccgtgacg
      901 ctgccctgct acctacaggt gcccaacatg gaggtgacgc atgtgtcaca gctgacttgg
      961 gcgcggcatg gtgaatctgg cagcatggcc gtcttccacc aaacgcaggg ccccagctat
     1021 tcggagtcca aacggctgga attcgtggca gccagactgg gcgcggagct gcggaatgcc
     1081 tcgctgagga tgttcgggtt gcgcgtagag gatgaaggca actacacctg cctgttcgtc
     1141 acgttcccgc agggcagcag gagcgtggat atctggctcc gagtgcttgc caagccccag
     1201 aacacagctg aggttcagaa ggtccagctc actggagagc cagtgcccat ggcccgctgc
     1261 gtctccacag ggggtcgccc gccagcccaa atcacctggc actcagacct gggcgggatg
     1321 cccaatacga gccaggtgcc agggttcctg tctggcacag tcactgtcac cagcctctgg
     1381 atattggtgc cctcaagcca ggtggacggc aagaatgtga cctgcaaggt ggagcacgag
     1441 agctttgaga agcctcagct gctgactgtg aacctcaccg tgtactaccc cccagaggta
     1501 tccatctctg gctatgataa caactggtac cttggccaga atgaggccac cctgacctgc
     1561 gatgctcgca gcaacccaga gcccacaggc tataattgga gcacgaccat gggtcccctg
     1621 ccaccctttg ctgtggccca gggcgcccag ctcctgatcc gtcctgtgga caaaccaatc
     1681 aacacaactt taatctgcaa cgtcaccaat gccctaggag ctcgccaggc agaactgacc
     1741 gtccaggtca aagagggacc tcccagtgag cactcaggca tatcccgtaa cgccatcatc
     1801 ttcctggttc tgggaatcct ggtttttctg atcctgctgg ggatcgggat ttatttctat
     1861 tggtccaaat gttcccgtga ggtcctttgg cactgtcatc tgtgtccctc gagtacagag
     1921 catgccagcg cctcagctaa tgggcatgtc tcctattcag ctgtgagcag agagaacagc
     1981 tcttcccagg atccacagac agagggcaca aggtgaattc aagggtgggc gcgccgaccc
     2041 agctttcttg tacaaagtgg ttgatctaga gggcccgcgg ttcgaaggta agcctatccc
     2101 taaccctcaa ttcgagcatc ttaccgccat ttattcccat atttgttctg tttttcttga
     2161 tttgggtata catttaaatg ttaataaaac aaaatggtgg ggcaatcatt tacattttat
     2221 gggatatgta attactagtt caggtgtatt gccacaagac aaacatgtta agaaactttc
     2281 ccgttattta cgctctgttc ctgttaatca acctctggat tacaaaattt gtgaaagatt
     2341 gactgatatt cttaactatg ttgctccttt tacgctgtgt ggatatgctg ctttaatgcc
     2401 tctgtatcat gctattgctt cccgtacggc tttcgttttc tcctccttgt ataaatcctg
     2461 gttgctgtct ctttatgagg agttgtggcc cgttgtccgt caacgtggcg tggtgtgctc
     2521 tgtgtttgct gacgcaaccc ccactggctg gggcattgcc accacctgtc aactcctttc
     2581 tgggactttc gctttccccc tcccgatcgc cacggcagaa ctcatcgccg cctgccttgc
     2641 ccgctgctgg acaggggcta ggttgctggg cactgataat tccgtggtgt tgtcggggaa
     2701 gctgacgtcc tttccatggc tgctcgcctg tgttgccaac tggatcctgc gcgggacgtc
     2761 cttctgctac gtcccttcgg ctctcaatcc agcggacctc ccttcccgag gccttctgcc
     2821 ggttctgcgg cctctcccgc gtcttcgctt tcggcctccg acgagtcgga tctccctttg
     2881 ggccgcctcc ccgcctgttt cgcctcggcg tccggtccgt gttgcttggt cgtcacctgt
     2941 gcagaattgc gaaccatgga ttccaccgtg aactttgtct cctggcatgc aaatcgtcaa
     3001 cttggcatgc caagaataat tcggatccaa gcttaggcct gctcgctttc ttgctgtccc
     3061 atttctatta aaggttcctt tgttccctaa gtccaactac taaactgggg gatattatga
     3121 agggccttga gcatctggat tctgcctagc gctaagctta acacgagcca tagatagaat
     3181 aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg
     3241 gcaagctagc ttaagtaacg ccattttgca aggcatggaa aatacataac tgagaataga
     3301 gaagttcaga tcaaggttag gaacagagag acagcagaat atgggccaaa caggatatct
     3361 gtggtaagca gttcctgccc cggctcaggg ccaagaacag ttggaacagc agaatatggg
     3421 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt
     3481 ccccagatgc ggtcccgccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc
     3541 cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc
     3601 ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg
     3661 cgcgccagtc ctccgataga ctgcgtcgcc cgggtacccg tgttctcaat aaaccctctt
     3721 gcagttgcat ccgactcgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga
     3781 ctgcccacct cgggggtctt tcattctcga gcagcttggc gtaatcatgg tcatagctgt
     3841 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     3901 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     3961 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     4021 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     4081 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     4141 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     4201 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     4261 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     4321 aggcgtttcg ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     4381 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     4441 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     4501 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     4561 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     4621 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     4681 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     4741 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     4801 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     4861 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     4921 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     4981 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     5041 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     5101 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     5161 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     5221 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     5281 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     5341 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     5401 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     5461 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     5521 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     5581 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     5641 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     5701 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     5761 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     5821 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     5881 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     5941 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     6001 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
     6061 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     6121 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     6181 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     6241 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat
     6301 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     6361 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
     6421 cacgacgttg taaaacgacg gccagtgaat tagtactcta gcttaagtaa cgccattttg
     6481 caaggcatgg aaaatacata actgagaata gagaagttca gatcaaggtt aggaacagag
     6541 agacagcaga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
     6601 ggccaagaac agttggaaca gcagaatatg ggccaaacag gatatctgtg gtaagcagtt
     6661 cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtcccgc cctcagcagt
     6721 ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct
     6781 tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag
     6841 ctcaataaaa gagcccacaa cccctcactc ggcgcgccag tcctccgata gact
//
