LOCUS       sequence-229310-         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses late endosome-targeted recruiter protein in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-FKBP-Rab7a-Blasticidin
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     21..45
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(110..112,113..433)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     primer_bind     complement(603..621)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
       61 ccacgctgtt ttgacctcca tagaagacac cgactctact agaggatcca tgggagtgca
      121 ggtggagact atctccccag gagacgggcg caccttcccc aagcgcggcc agacctgcgt
      181 ggtgcactac accgggatgc ttgaagatgg aaagaaattt gattcctccc gggacagaaa
      241 caagcccttt aagtttatgc taggcaagca ggaggtgatc cgaggctggg aagaaggggt
      301 tgcccagatg agtgtgggtc agagagccaa actgactata tctccagatt atgcctatgg
      361 tgccactggg cacccaggca tcatcccacc acatgccact ctcgtcttcg atgtggagct
      421 tctaaaactg gaaggtgctg cacttctgcc cggttctatg gtgagcaagg gcgaggagga
      481 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa
      541 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
      601 cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc
      661 tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt
      721 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
      781 cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa
      841 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg ca
//
