LOCUS       sequence-229313-         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mitochondrial membrane-targeted recruiter protein in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-FKBP-BclXL-Blasticidin
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(112..130)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     380..399
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     regulatory      684..693
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             696..722
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt
       61 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
      121 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt
      181 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct
      241 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
      301 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca
      361 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct
      421 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc
      481 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg
      541 tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg
      601 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg
      661 acgagctgta caagcccggt tctgccgcca tggcatatcc atatgacgtc ccagactatg
      721 ctgccgagag ccgaaagggc caggaacgct tcaaccgctg gttcctgacg ggcatgactg
      781 tggccggcgt ggttctgctg ggctcactct tcagtcggaa atgactcgag tctagagggc
      841 ccgacgcgtc tggccaccgg tcgc
//
