LOCUS       sequence-229317-        7308 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  eukaryotic expression of Flag tagged p110a WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7308)
  AUTHORS   Fritsch R, de Krijger I, Fritsch K, George R, Reason B, Kumar MS, 
            Diefenbacher M, Stamp G, Downward J
  TITLE     RAS and RHO families of GTPases directly regulate distinct 
            phosphoinositide 3-kinase isoforms.
  JOURNAL   Cell. 2013 May 23;153(5):1050-63. doi: 10.1016/j.cell.2013.04.031.
  PUBMED    23706742
REFERENCE   2  (bases 1 to 7308)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.04"; journalName: "Cell"; date: "2013-05-23- 
            23"; volume: "153"; issue: "5"; pages: "1050-63"
COMMENT     Sequence Label: pSG5-p110-alpha-WT
FEATURES             Location/Qualifiers
     source          1..7308
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        76..286
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        98..294
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      145..280
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          325..897
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     905..924
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     952..971
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        952..970
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      985..994
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             994..1017
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    4245..4366
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4282..4301)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4336..4355
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4400..4417)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4571..5159)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4651..4670)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5330..6121,6122..6190))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5953..5972
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6191..6295)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6363..6381
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6419..6441)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6541..6560
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(6627..7082)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6764..6785)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6976..6995
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7208..7230
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7222..7239
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7223..7239
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7276..7296)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaggaaag
       61 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
      121 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
      181 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
      241 gagctattcc agaagtagtg aagaggcttt tttggaggcc taggcttttg caaaaagctc
      301 cggatcgatc ctgagaactt cagggtgagt ttggggaccc ttgattgttc tttctttttc
      361 gctattgtaa aattcatgtt atatggaggg ggcaaagttt tcagggtgtt gtttagaatg
      421 ggaagatgtc ccttgtatca ccatggaccc tcatgataat tttgtttctt tcactttcta
      481 ctctgttgac aaccattgtc tcctcttatt ttcttttcat tttctgtaac tttttcgtta
      541 aactttagct tgcatttgta acgaattttt aaattcactt ttgtttattt gtcagattgt
      601 aagtactttc tctaatcact tttttttcaa ggcaatcagg gtatattata ttgtacttca
      661 gcacagtttt agagaacaat tgttataatt aaatgataag gtagaatatt tctgcatata
      721 aattctggct ggcgtggaaa tattcttatt ggtagaaaca actacatcct ggtcatcatc
      781 ctgcctttct ctttatggtt acaatgatat acactgtttg agatgaggat aaaatactct
      841 gagtccaaac cgggcccctc tgctaaccat gttcatgcct tcttcttttt cctacagctc
      901 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt gtaatacgac
      961 tcactatagg gcgaattcgg atccgccacc atggattaca aggatgacga cgataagcct
     1021 ccacgaccat cttcgggtga actgtggggc atccacttga tgcccccacg aatcctagtg
     1081 gaatgtttac tccccaatgg aatgatagtg actttagaat gcctccgtga ggccacactc
     1141 gtcaccatca aacatgaact gttcagagag gccaggaaat accctctcca tcagcttctg
     1201 caagacgaaa cttcttacat tttcgtaagt gtcacccaag aagcagaaag ggaagaattt
     1261 tttgatgaaa caagacgact ttgtgacctt cggctttttc aacccttttt aaaagttatt
     1321 gaaccagtag gcaaccgtga agaaaagatc ctcaatcgag aaattggttt tgttattggc
     1381 atgccagtgt gtgaatttga tatggttaaa gatccagaag tccaagactt tcgaaggaac
     1441 attctgaatg tttgcaaaga agctgtggac ctgcgggatc tcaactcgcc tcatagcaga
     1501 gcaatgtatg tctaccctcc aaatgtcgag tcttccccag aactgccaaa gcacatctac
     1561 aacaagttag ataaaggaca aatcatagtg gtgatttggg taatagtctc tccaaacaac
     1621 gacaagcaga agtacactct gaagatcaat catgactgtg tgccagagca agtcattgct
     1681 gaagccatca ggaaaaagac tcggagcatg ttgttgtcct ctgagcagct gaaactctgt
     1741 gtcttagaat atcagggcaa gtatattctg aaagtgtgtg gctgtgacga atacttcctg
     1801 gaaaagtacc ctctgagtca gtacaagtac ataagaagct gtataatgct ggggaggatg
     1861 cccaacttga tgctgatggc caaagaaagc ctatactctc agctgccgat tgatagcttc
     1921 accatgccgt catactccag gcgcatctcc acagccacac cctacatgaa tggagagaca
     1981 tctacgaaat ccctctgggt cataaatagt gcgctcagaa taaaaattct ttgtgcaacc
     2041 tatgtaaatg taaatattcg agacattgat aagatctatg ttcgaacagg tatctaccat
     2101 ggaggagaac ccttatgtga caatgtgaac actcaaagag taccttgttc caatcctagg
     2161 tggaatgaat ggctgaatta tgatatatac attcctgatc ttcctcgtgc tgcacgcctt
     2221 tgcctttcaa tctgctctgt taaaggccga aagggtgcta aggaggagca ctgtccgttg
     2281 gcctggggaa acataaactt gtttgattat acagacaccc tagtgtccgg gaaaatggct
     2341 ttgaatctct ggcctgtacc gcatgggtta gaagatctgc tgaaccctat tggtgttact
     2401 gggtcaaatc caaataaaga aactccatgc ttagagttgg agtttgattg gttcagcagt
     2461 gtggtgaagt ttccagacat gtctgtgatc gaagaacatg ccaattggtc cgtgtcccga
     2521 gaagctggat tcagttactc ccatacagga ctgagtaaca gactagccag agacaatgag
     2581 ttaagagaaa atgacaagga acagctccga gcactttgca cccgggaccc actatctgaa
     2641 atcactgaac aagagaaaga cttcctatgg agccacagac actactgcgt aactattcct
     2701 gaaatcctac ccaaattgct tctgtctgtc aagtggaatt ccagagacga agtggcccag
     2761 atgtactgct tagtaaaaga ttggcctcca atcaaaccag agcaagccat ggaactcctg
     2821 gactgtaact atccagatcc tatggttcgg agttttgctg ttcggtgctt agaaaaatat
     2881 ttaacagatg acaaactttc tcagtacctc attcaacttg tacaggtctt aaaatatgaa
     2941 cagtatttgg ataacctgct tgtgagattt ttactcaaga aagcattgac aaatcaaagg
     3001 attggccatt ttttcttttg gcatttaaaa tctgagatgc acaataagac tgtcagtcag
     3061 aggtttggcc tgctattgga gtcctactgc cgtgcctgtg ggatgtatct gaagcacctg
     3121 aacagacaag tagaggccat ggagaagctc atcaacctaa cggacatcct taagcaggag
     3181 aagaaggatg agacacaaaa ggtacagatg aagtttttgg ttgaacagat gagacagcca
     3241 gacttcatgg atgctttgca gggttttctg tcccctctga atcctgctca ccaactagga
     3301 aacctcaggc ttgaagagtg tcgaattatg tcctctgcaa aaaggccact gtggttgaat
     3361 tgggagaacc cagacatcat gtcagagcta ctgtttcaga acaatgagat catctttaaa
     3421 aatggcgacg acttacggca agatatgtta acccttcaga tcatccgaat catggagaac
     3481 atctggcaaa accaaggcct tgaccttcgc atgctacctt atggctgtct atccattggg
     3541 gactgtgtgg gtctcatcga ggtggtgaga aactctcaca ccatcatgca aatccagtgc
     3601 aaaggaggcc tgaagggggc gctgcagttc aacagccaca cactgcatca atggctcaag
     3661 gacaagaaca agggcgagat atatgatgca gccattgacc tgttcactcg gtcctgcgct
     3721 gggtactgcg tggcaacctt tatcttggga attggagacc ggcacaacag caacatcatg
     3781 gtgaaagatg acggacagct gtttcatata gattttgggc actttttgga tcacaagaag
     3841 aaaaaatttg gctataagcg ggaacgtgtg ccatttgtgt tgacacagga tttcttgatt
     3901 gtgattagta agggagcaca agagtacacc aagaccagag agtttgagag gtttcaggag
     3961 atgtgttaca aggcttacct agcaattcgg cagcatgcca atctcttcat caaccttttt
     4021 tcaatgatgc ttggctctgg aatgccagaa ctacaatctt ttgatgacat tgcatatatc
     4081 cgaaagactc tagccttgga caaaactgag caagaagctt tggaatattt cacaaagcaa
     4141 atgaatgatg cacatcatgg tggatggacg acaaaaatgg attggatctt ccacaccatc
     4201 aagcagcatg ctttgaactg aggatccaga tcttattaaa gcagaacttg tttattgcag
     4261 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     4321 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggtcga
     4381 ctctagactc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     4441 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     4501 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     4561 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     4621 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     4681 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     4741 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     4801 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     4861 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     4921 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     4981 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     5041 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     5101 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     5161 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     5221 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     5281 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     5341 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     5401 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     5461 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     5521 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     5581 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     5641 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     5701 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     5761 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     5821 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     5881 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     5941 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     6001 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     6061 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     6121 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     6181 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     6241 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     6301 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     6361 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
     6421 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     6481 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     6541 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     6601 gcgtaaggag aaaataccgc atcaggaaat tgtaatcgtt aatattttgt taaaattcgc
     6661 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     6721 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     6781 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     6841 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     6901 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     6961 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     7021 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     7081 gtccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     7141 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
     7201 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtcgac ttctgaggcg
     7261 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtccc
//
