LOCUS       sequence-229319-         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ER-targeted recruiter protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-TCRb-FKBP-Blasticidin
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        4..48
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     primer_bind     45..69
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             200..244
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
ORIGIN
        1 gcaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
       61 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgactc
      121 tactagagga tccatggact cctggacctt ctgctgtgtg tccctttgca tcctggtagc
      181 gaagcataca gatgctggag gcctgaacga catcttcgag gctcagaaaa tcgaatggca
      241 cgaaggcagc gttatccagt caccccgcca tgaggtgaca gagatgggac aagaagtgac
      301 tctgagatgt aaaccaattt caggccacaa ctcccttttc tggtacagac agaccatgat
      361 gcggggactg gagttgctca tttactttaa caacaacgtt ccgatagatg attcagggat
      421 gcccgaggat cgattctcag ctaagatgcc taatgcatca ttctccactc tgaagatcca
      481 gccctcagaa cccagggact cagctgtgta cttctgtgcc agcagtttct cgacctgttc
      541 ggctaactat ggctacacct tcggttcggg gaccaggtta accgttgtag aggacctgaa
      601 caaggtgttc ccacccgagg tcgctgtgtt tgagccatca gaagcagaaa tctcccacac
      661 ccaaaaggcc acactggtgt gcctggccac aggcttcttc cccgaccacg tggagctgag
      721 ctggtgggtg aatgggaagg aggtgcacag tggggtcagc acagacccgc agcccctcaa
      781 ggagcagccc gccctcaatg actccagata ctgcctgagc agccgcctga gggtctcggc
      841 caccttctgg cagaaccccc gcaaccactt ccgctgtcaa gtccagttct acgggctctc
      901 ggagaatgac
//
