LOCUS       sequence-22932-e         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  TITLE     Other Plasmids
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: M12 pCarGFP2
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3..719
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="suitable for expression in plants due to removal of 
                     a cryptic intron"
                     /translation="MSKGEELFTGVVPILVELDGDVNGYKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKTRHNIEDGGVQLADHYQQNTPIGDDPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(33..61)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     656..676
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(781..800)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ccatgagtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
       61 gtgatgttaa tgggtacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg
      121 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac
      181 ttgtcactac tctcacttat ggtgttcaat gcttttcaag atatccagat catatgaagc
      241 ggcacgactt cttcaagagc gccatgcctg agggatacgt gcaggagagg accatcttct
      301 tcaaggacga cgggaactac aagacacgtg ctgaagtcaa gtttgaggga gacaccctcg
      361 tcaacaggat cgagcttaag ggaatcgatt tcaaggagga cggaaacatc ctcggccaca
      421 agttggaata caactacaac tcccacaacg tatacatcat ggccgacaag caaaagaacg
      481 gcatcaaagc caacttcaag acccgccaca acatcgaaga cggcggcgtg caactcgctg
      541 atcattatca acaaaatact ccaattggcg atgaccctgt ccttttacca gacaaccatt
      601 acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc
      661 ttcttgagtt tgtaacggct gctgggatta cacatggcat ggatgaacta tacaaataac
      721 tcgagcctct agaactatag tgagtcgtat tacgtagatc cagacatgat aagatacatt
      781 gatgagtttg gacaaaccac aact
//
