LOCUS       sequence-229320-         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ER-targeted recruiter protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-TCRb-FKBP-Blasticidin
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     44..63
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     regulatory      348..357
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             360..386
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(387..389,390..713)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
ORIGIN
        1 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
       61 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
      121 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
      181 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
      241 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
      301 gccgccactc caccggcggc atggacgagc tgtacaagcc cggttctgcc gccatggcat
      361 atccatatga cgtcccagac tatgctatgg gagtgcaggt ggagactatc tccccaggag
      421 acgggcgcac cttccccaag cgcggccaga cctgcgtggt gcactacacc gggatgcttg
      481 aagatggaaa gaaatttgat tcctcccggg acagaaacaa gccctttaag tttatgctag
      541 gcaagcagga ggtgatccga ggctgggaag aaggggttgc ccagatgagt gtgggtcaga
      601 gagccaaact gactatatct ccagattatg cctatggtgc cactgggcac ccaggcatca
      661 tcccaccaca tgccactctc gtcttcgatg tggagcttct aaaactggaa taactcgagt
      721 ctagagggcc cgacgcgtct ggccaccggt cgcccct
//
