LOCUS       sequence-229325-       13294 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor vector for FLPe recombinase-mediated cassette exchange in 
            master cell lines created with plasmid #112666. This vector allows 
            OCT4 dependent expression of GFP, and contains cHS4 insulators..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13294)
  AUTHORS   Ordovas L, Boon R, Pistoni M, Chen Y, Wolfs E, Guo W, Sambathkumar 
            R, Bobis-Wozowicz S, Helsen N, Vanhove J, Berckmans P, Cai Q, 
            Vanuytsel K, Eggermont K, Vanslembrouck V, Schmidt BZ, Raitano S, 
            Van Den Bosch L, Nahmias Y, Cathomen T, Struys T, Verfaillie CM
  TITLE     Efficient Recombinase-Mediated Cassette Exchange in hPSCs to Study 
            the Hepatocyte Lineage Reveals AAVS1 Locus-Mediated Transgene 
            Inhibition.
  JOURNAL   Stem Cell Reports. 2015 Nov 10;5(5):918-931. doi: 
            10.1016/j.stemcr.2015.09.004. Epub 2015 Oct 8.
  PUBMED    26455413
REFERENCE   2  (bases 1 to 13294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2015.09.004"; journalName: "Stem Cell Reports"; 
            date: "2015-11-10- 10"; volume: "5"; issue: "5"; pages: "918-931"
COMMENT     Sequence Label: pZ P (cHS4)X4 OCT4p eGFP SV40pA v2
FEATURES             Location/Qualifiers
     source          1..13294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..837
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     protein_bind    complement(847..880)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     regulatory      6201..6210
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6207..6209,6210..6212,6213..6926)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6207..6209,6210..6212,6213..6923)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6252..6273)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6513..6532)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6860..6881
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7049..7130
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7086..7105)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7140..7159
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    8598..8709
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8698..8715
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(8863..9462)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8946..8966)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     9443..9462
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(9472..9525)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    9546..9571
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    complement(9575..9608)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     misc_feature    9615..10418
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(10432..10451)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10433..10451)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10461..10478)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10461..10477)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10470..10492)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10619..11074
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10706..10725)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10916..10937
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11100..11204
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11205..11273,11274..12065)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11423..11442)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12236..12824
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12725..12744
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12978..12995
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13112..13133
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13148..13153,13154..13171,13172..13178)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13186..13202
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13191..13213
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13210..13226
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13210..13226
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13245..13265
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13247..13265
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agagcagagc caggaacccc tgtagggaag gggcaggaga gccaggggca tgagatggtg
       61 gacgaggaag ggggacaggg aagcctgagc gcctctcctg ggcttgccaa ggactcaaac
      121 ccagaagccc agagcagggc cttagggaag cgggaccctg ctctgggcgg aggaatatgt
      181 cccagatagc actggggact ctttaaggaa agaaggatgg agaaagagaa agggagtaga
      241 ggcggccacg acctggtgaa cacctaggac gcaccattct cacaaaggga gttttccaca
      301 cggacacccc cctcctcacc acagccctgc caggacgggg ctggctactg gccttatctc
      361 acaggtaaaa ctgacgcacg gaggaacaat ataaattggg gactagaaag gtgaagagcc
      421 aaagttagaa ctcaggacca acttattctg attttgtttt tccaaactgc ttctcctctt
      481 gggaagtgta aggaagctgc agcaccagga tcagtgaaac gcaccagaca gccgcgtcag
      541 agcagctcag gttctgggag agggtagcgc agggtggcca ctgagaaccg ggcaggtcac
      601 gcatcccccc cttccctccc accccctgcc aagctctccc tcccaggatc ctctctggct
      661 ccatcgtaag caaaccttag aggttctggc aaggagagag atggctccag gaaatggggg
      721 tgtgtcacca gataaggaat ctgcctaaca ggaggtgggg gttagaccca atatcaggag
      781 actaggaagg aggaggccta aggatggggc ttttctgtca ccaatcctgt ccctagtagc
      841 ggccgcgaag ttcctatact ttctagagaa taggaacttc ttcgatctcg acaagcgtat
      901 cgatacgggg acagcccccc cccaaagccc ccagggatgt aattacgtcc ctcccccgct
      961 agggggcagc agcgagccgc ccggggctcc gctccggtcc ggcgctcccc ccgcatcccc
     1021 gagccggcag cgtgcgggga cagcccgggc acggggaagg tggcacggga tcgctttcct
     1081 ctgaacgctt ctcgctgctc tttgagcctg cagacacctg gggggatacg gggaaaaagc
     1141 tttaggcttg tgtctgagcc tgcatgtttg atggtgtctg gatgcaagca gaaggggtgg
     1201 aagagcttgc ctggagagat acagctgggt cagtaggact gggacaggca gctggagaat
     1261 tgccatgtag atgttcatac aatcgtcaaa tcatgaaggc tggaaaagcc ctccaagatc
     1321 cccaagacca accccaaccc acccaccgtg cccactggcc atgtccctca gtgccacatc
     1381 cccacagttc ttcatcacct ccagggacgg tgaccccccc acctccgtgg gcagctgtgc
     1441 cactgcagca ccgctctttg gagaaggtaa atcttgctaa atccagcccg accctcccct
     1501 ggcacaacgt aaggccatta tctctcatcc aactccagga cggagtcagt gagatgcata
     1561 cggggacagc ccccccccaa agcccccagg gatgtaatta cgtccctccc ccgctagggg
     1621 gcagcagcga gccgcccggg gctccgctcc ggtccggcgc tccccccgca tccccgagcc
     1681 ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca cgggatcgct ttcctctgaa
     1741 cgcttctcgc tgctctttga gcctgcagac acctgggggg atacggggaa aaagctttag
     1801 gcttgtgtct gagcctgcat gtttgatggt gtctggatgc aagcagaagg ggtggaagag
     1861 cttgcctgga gagatacagc tgggtcagta ggactgggac aggcagctgg agaattgcca
     1921 tgtagatgtt catacaatcg tcaaatcatg aaggctggaa aagccctcca agatccccaa
     1981 gaccaacccc aacccaccca ccgtgcccac tggccatgtc cctcagtgcc acatccccac
     2041 agttcttcat cacctccagg gacggtgacc cccccacctc cgtgggcagc tgtgccactg
     2101 cagcaccgct ctttggagaa ggtaaatctt gctaaatcca gcccgaccct cccctggcac
     2161 aacgtaaggc cattatctct catccaactc caggacggag tcagtgagat cgatcaaatg
     2221 cgcctcgagc tcaagcttcc catgtcaagt aagtggggtg ggtagatggg agggcatatg
     2281 agagcggggc agcaatttta tgtcttagtt ggaaggaagt ttagagttca ttgagtccaa
     2341 atcctcttta ctaggtgagg aaactgaggc acaggagggg gaggtgccat tagccaggga
     2401 gtcagagcca ggacttagaa cctggtccat gcctcctagt ttgttcctga ccaatcctcc
     2461 cagctcaccc actccttttg ctctcacctc tgactcccta caggctacag ctagagagtt
     2521 gactcctcta tttgaggtaa gagtgtctgg atttgggata aggtgacctc agtttgcagg
     2581 tctcatgggg gagatcagag ggtcctttgt tgagaagagg ggacttggtc cacagtgagc
     2641 aaaggaattt gtctaaattg ctattaaggg agatcatctc ccaaggtggg ccaagcctga
     2701 accctcagcc ctccatttct tccctttctt ttgccttcag attgacaggc ctcggaagtc
     2761 aaaataagtg gtttcctaga ccgggtcgag agcaagtctc tattggtccc aactgagttt
     2821 tttcagctgg tttttcaacc aaacagcacc tcatctccca gtgaggggaa gggaaggctg
     2881 ggctgagagc agcaaggctg ctcatctcac ctctccccac ccagccatgc cagccgccac
     2941 acctggtggg gagaggtggg cctcacctgg gtcccctggc agtgctctgt gaagggtctt
     3001 gacattgcac tgtaataata aaggtgtgtg tgaagtatct ttttatggtg actttctaaa
     3061 acccagggaa tcatgggacc agttctgatg actcagcctg gcttccagtc tcctccaggc
     3121 ccaacggtgg cccccagcac tggttggggc ctgggagagc tggccttggc tgaagtgaag
     3181 ccacctaccc ttcaggcata acaggacagt gagaaggaag gaaagcctgc ctcaacctcc
     3241 catcagccct gagcacccca gaagggggcc ggctaggagt ctaggcatgc aggaggctga
     3301 cccctgactg ggctcatatc cagccacaag gcagccaggg acccgggcac ccaccccttg
     3361 tctgcgtccc tctcgggaat gggcctcttg cccaggccag aaatacacca cctacagtac
     3421 aaattataat ctaaaaacaa gagggtggtg ttgagtgggg aaattgggga aggtgtttta
     3481 ggagccacta ggaaaatggg cagcagggac tctctggact ggcttgggaa gagcgctttt
     3541 ggggaacctg gaggatggca agctgagaaa cactggtgtg gagattccag ccaaatccca
     3601 ggcctgcccc tccccctcct ctgagaggcc gtcttcttgg cagacagcag agagatgcat
     3661 gacaaaggtg ccgtgatggt tctgtcctgg ggattgagat ggctggggag gggcctcctc
     3721 ctgttccgaa gcatgttcct cccaccccca ccaggcccca taatctacct gccttttggg
     3781 cagttaaagg ccgagaagtg aacacagctg caaccccact gccttgtaga ccttccggca
     3841 gacctgtggc aggtattgaa atgcacgcat acaattaggc tcaaaaagtc tacacagaca
     3901 ggagatgggc acacgaacag aggcaacata agagtggggg aaaagtctca aaagactcac
     3961 ggatgccacc aagatgaaga cagctggcca cgggacaccc atccccttag aaggcagata
     4021 gagccactga ccccagcaga caagcccagg cagggctgag cctggagcct gcaatgagaa
     4081 gccttactta agtcgacaga ggtcagcgtg cccagtccag acctggcctt ctggccttcg
     4141 aagctgtggg gagccctggc ccagagcccc ctctggagcc cccagactta ccccaggccc
     4201 tccactgaga tcaagttttg ggagcagaca gacaaacatc atccctcaca gacaggcatt
     4261 ccgttggcta ttctcttgca aacagaatca agcactagac cagcagcatg agcctcagga
     4321 tactcaggcc gggcccagaa aaacagaccc tgaaggggag cttagggcag ccttcctgca
     4381 cccctccaca aatcactctc cacctcctct gcgtctttct gccagccagc cccactaaac
     4441 aaagcacatc cctcaatctg ccgggctcgg ggagggatgc acgatgaagc tggacgcctg
     4501 agtcccccag aggaaggagg aactagatac ctaggtccct gtggggggcc cttggtgccc
     4561 gtctgaggct cagtctttga ggggattgca gaggggggtt gctggagctc cttttagcgt
     4621 ctctgaaggg gattctgtgt gaggggattg ggactggggg gttggggagc aggaagcagt
     4681 ccccagggga gccatccagg cccattcaag ggttgagcac ttgtttaggg ttagagctgc
     4741 cccctctggg gaccgggatt gtccagccaa ggccattgtc ctgccccctt cccccagtcc
     4801 ctcccaggct tctttgaacc tgaagtcaga tattttttct ccacaccccc caccccctgg
     4861 ttttccccac ccagggccta gggctggagg cctgggccag ggaggtgggg gagggagaac
     4921 ggggcctacc gtggtattag atgtctgagt tttggttgag aggggagcaa ggaacctgat
     4981 gtgcaggttc catagtggag ggggcccaaa gcgggtgtct tatcactctg tttcagcaaa
     5041 ggttggtaaa ctgaggccca gtcagtccaa agtctggtcc cttgaagggg aagtagggac
     5101 caacccctta gtctgttaga tgaggagagt ctggagtctg attctggaag acggaggggt
     5161 ggggggatgg ggggtggggg gatatagcac ggaggccttg tctggcagtc tactcttgaa
     5221 gatggggtga aatttggcag gctgggcaga tggtgccagg cacccaggct gcggggtggc
     5281 tggatttggc cagtatcggg atgggaatgc ctaggattct ggatggatcg ggggaaggca
     5341 taagggagca gctggccatt gtgcttatgg ctgttgatgc attgagggat agcgccacac
     5401 acacattcaa taaatttgag gagctgagag ggtgactggc ccctgaaggc acagtgccag
     5461 aggtctgtgg agggggggtc aagcacctgg gttcctgaag aacatggagg tgtgggagtg
     5521 attccagaca gctgggatgt gcagagcctg agagagtgcc agggagcggg ttgggagttg
     5581 aaagttgggt gtggtggctc acgcctttaa tcatgacact gggcggcaga ggcgggagga
     5641 tttcttgagg acaggaattc aagaccagcc tgggtaacat agcaaggccc catctctact
     5701 aaaaataaaa aaactaacag ggcacagtgg tccaagcctg tagtcccagc cacttaggag
     5761 gctggagcag aaggattgct ttggcccagt agatcgaggc tacattgagc catcattgta
     5821 ctccactgca ctccagtctg ggcaacaaag tgagaccctg tcttaaaaaa taaaaataaa
     5881 aaaagtttct gtgggggacc tgcactgagg tcctggaggg gcgccagttg tgtctcccgg
     5941 ttttcccctt ccacagacac cattgccacc accattaggc aaacatcctt cgcctcagtt
     6001 tctcccccca cctccctctc ctccacccat ccagggggcg gggccagagg tcaaggctag
     6061 tgggtgggac tggggaggga gagaggggtt gagtagtccc ttcgcaagcc ctcatttcac
     6121 caggcccccg gcttggggcg ccttccttcc ccatggcggg acacctggct tcagattttg
     6181 ccttctcgga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
     6241 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     6301 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     6361 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     6421 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     6481 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     6541 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     6601 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     6661 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     6721 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     6781 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     6841 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     6901 tcggcatgga cgagctgtac aagtaaagcg gccgcgactc tagatcataa tcagccatac
     6961 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     7021 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     7081 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     7141 tggtttgtcc aaactcatca atgtatccga agcgcatttg atcgatctca ctgactccgt
     7201 cctggagttg gatgagagat aatggcctta cgttgtgcca ggggagggtc gggctggatt
     7261 tagcaagatt taccttctcc aaagagcggt gctgcagtgg cacagctgcc cacggaggtg
     7321 ggggggtcac cgtccctgga ggtgatgaag aactgtgggg atgtggcact gagggacatg
     7381 gccagtgggc acggtgggtg ggttggggtt ggtcttgggg atcttggagg gcttttccag
     7441 ccttcatgat ttgacgattg tatgaacatc tacatggcaa ttctccagct gcctgtccca
     7501 gtcctactga cccagctgta tctctccagg caagctcttc caccccttct gcttgcatcc
     7561 agacaccatc aaacatgcag gctcagacac aagcctaaag ctttttcccc gtatcccccc
     7621 aggtgtctgc aggctcaaag agcagcgaga agcgttcaga ggaaagcgat cccgtgccac
     7681 cttccccgtg cccgggctgt ccccgcacgc tgccggctcg gggatgcggg gggagcgccg
     7741 gaccggagcg gagccccggg cggctcgctg ctgcccccta gcgggggagg gacgtaatta
     7801 catccctggg ggctttgggg gggggctgtc cccgtatgca tctcactgac tccgtcctgg
     7861 agttggatga gagataatgg ccttacgttg tgccagggga gggtcgggct ggatttagca
     7921 agatttacct tctccaaaga gcggtgctgc agtggcacag ctgcccacgg aggtgggggg
     7981 gtcaccgtcc ctggaggtga tgaagaactg tggggatgtg gcactgaggg acatggccag
     8041 tgggcacggt gggtgggttg gggttggtct tggggatctt ggagggcttt tccagccttc
     8101 atgatttgac gattgtatga acatctacat ggcaattctc cagctgcctg tcccagtcct
     8161 actgacccag ctgtatctct ccaggcaagc tcttccaccc cttctgcttg catccagaca
     8221 ccatcaaaca tgcaggctca gacacaagcc taaagctttt tccccgtatc cccccaggtg
     8281 tctgcaggct caaagagcag cgagaagcgt tcagaggaaa gcgatcccgt gccaccttcc
     8341 ccgtgcccgg gctgtccccg cacgctgccg gctcggggat gcggggggag cgccggaccg
     8401 gagcggagcc ccgggcggct cgctgctgcc ccctagcggg ggagggacgt aattacatcc
     8461 ctgggggctt tggggggggg ctgtccccgt atcgatacgc ttactagtgt cgagtgctat
     8521 tgtcttccca atcctccccc ttgctgtcct gccccacccc accccccaga atagaatgac
     8581 acctactcag acaatgcgat gcaatttcct cattttatta ggaaaggaca gtgggagtgg
     8641 caccttccag ggtcaaggaa ggcacggggg aggggcaaac aacagatggc tggcaactag
     8701 aaggcacagt cgaggctgat cagcgggttt aaacgggccc tctagagtcg gtgggcctcg
     8761 ggggcgggtg cggggtcggc ggggccgccc cgggtggctt cggtcggagc catggggtcg
     8821 tgcgctcctt tcggtcgggc gctgcgggtc gtggggcggg cgtcaggcac cgggcttgcg
     8881 ggtcatgcac caggtgcgcg gtccttcggg cacctcgacg tcggcggtga cggtgaagcc
     8941 gagccgctcg tagaagggga ggttgcgggg cgcggaggtc tccaggaagg cgggcacccc
     9001 ggcgcgctcg gccgcctcca ctccggggag cacgacggcg ctgcccagac ccttgccctg
     9061 gtggtcgggc gagacgccga cggtggccag gaaccacgcg ggctccttgg gccggtgcgg
     9121 cgccaggagg ccttccatct gttgctgcgc ggccagccgg gaaccgctca actcggccat
     9181 gcgcgggccg atctcggcga acaccgcccc cgcttcgacg ctctccggcg tggtccagac
     9241 cgccaccgcg gcgccgtcgt ccgcgaccca caccttgccg atgtcgagcc cgacgcgcgt
     9301 gaggaagagt tcttgcagct cggtgacccg ctcgatgtgg cggtccgggt cgacggtgtg
     9361 gcgcgtggcg gggtagtcgg cgaacgcggc ggcgagggtg cgtacggccc gggggacgtc
     9421 gtcgcgggtg gcgaggcgca ccgtgggctt gtactcggtc atttcgagcc tagggccggg
     9481 attctcctcc acgtcaccgc atgttagaag acttcctctg ccctctccgc tgccagatct
     9541 gaggccctgt gggaggaaga gaagaggtca ggccgaagtt cctatactat ttgaagaata
     9601 ggaacttcaa gcttgcccca ctgtggggtg gaggggacag ataaaagtac ccagaaccag
     9661 agccacatta accggccctg ggaatataag gtggtcccag ctcggggaca caggatccct
     9721 ggaggcagca aacatgctgt cctgaagtgg acataggggc ccgggttgga ggaagaagac
     9781 tagctgagct ctcggacccc tggaagatgc catgacaggg ggctggaaga gctagcacag
     9841 actagagagg taaggggggt aggggagctg cccaaatgaa aggagtgaga ggtgacccga
     9901 atccacagga gaacggggtg tccaggcaaa gaaagcaaga ggatggagag gtggctaaag
     9961 ccagggagac ggggtacttt ggggttgtcc agaaaaacgg tgatgatgca ggcctacaag
    10021 aaggggaggc gggacgcaag ggagacatcc gtcggagaag gccatcctaa gaaacgagag
    10081 atggcacagg ccccagaagg agaaggaaaa gggaacccag cgagtgaaga cggcatgggg
    10141 ttgggtgagg gaggagagat gcccggagag gacccagaca cggggaggat ccgctcagag
    10201 gacatcacgt ggtgcagcgc cgagaaggaa gtgctccgga aagagcatcc ttgggcagca
    10261 acacagcaga gagcaagggg aagagggagt ggaggaagac ggaacctgaa ggaggcggca
    10321 gggaaggatc tgggccagcc gtagaggtga cccaggccac aagctgcaga cagaaagcgg
    10381 cacaggccca ggggagagaa tgctggtcag agaaagcagg tacccaattc gccctatagt
    10441 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
    10501 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
    10561 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
    10621 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
    10681 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
    10741 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
    10801 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
    10861 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
    10921 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
    10981 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
    11041 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
    11101 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
    11161 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
    11221 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
    11281 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
    11341 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
    11401 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
    11461 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
    11521 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
    11581 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
    11641 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
    11701 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
    11761 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
    11821 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
    11881 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
    11941 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
    12001 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
    12061 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    12121 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    12181 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    12241 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
    12301 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    12361 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
    12421 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
    12481 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    12541 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    12601 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    12661 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    12721 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    12781 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    12841 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
    12901 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
    12961 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
    13021 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
    13081 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
    13141 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
    13201 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
    13261 aaagggaaca aaagctggag ctccaccgcg gtgg
//
