LOCUS       sequence-229328-       11742 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express 3xNLS-Klf4 TALE-GCN4 engineered to bind a site in the human 
            KLF4 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11742)
  AUTHORS   Zhao C, Zhang Y, Zhao Y, Ying Y, Ai R, Zhang J, Wang Y
  TITLE     Multiple Chemical Inducible Tal Effectors for Genome Editing and 
            Transcription Activation.
  JOURNAL   ACS Chem Biol. 2018 Mar 16;13(3):609-617. doi: 
            10.1021/acschembio.7b00606. Epub 2018 Jan 8.
  PUBMED    29308880
REFERENCE   2  (bases 1 to 11742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.7b00606"; journalName: "ACS Chem Biol"; date: 
            "2018-03-16- 16"; volume: "13"; issue: "3"; pages: "609-617"
COMMENT     Sequence Label: EF1a-3NLS-Klf4 TALE-GCN4
FEATURES             Location/Qualifiers
     source          1..11742
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        133..344
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      1208..1217
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1229..1249
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1253..1273
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1277..1297
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3917..3973
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3989..4045
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4061..4117
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4133..4189
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4205..4261
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4277..4333
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4349..4405
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4421..4477
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4493..4549
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4565..4621
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     primer_bind     complement(4845..4865)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4850..5179
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5030..5165
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5189..5563
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    5649..6237
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5702..5722)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6120..6131)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6324..6557
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6629..6750
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      6769..6904
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6936..6955)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6937..6955)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6965..6982)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6965..6981)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6974..6996)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7126..7581
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7213..7232)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7423..7444
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7610..7714
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7715..7783,7784..8575)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7933..7952)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8746..9334
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9235..9254
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9488..9505
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9622..9643
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9658..9663,9664..9681,9682..9688)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9696..9712
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9701..9723
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9720..9736
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9720..9736
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9755..9775
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9757..9775
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             10031..10211
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10255..10380
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10873..11106
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11291..11335
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11602..11719
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctagcccggg ctcgatcgac atatgataag ctttgcaaag atggataaag ttttaaacag
       61 agaggaatct ttgcagctaa tggaccttct aggtcttgaa aggagtggga attggctccg
      121 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
      181 tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
      241 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
      301 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg
      361 gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca
      421 cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt
      481 cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc
      541 gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata
      601 agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata
      661 gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg
      721 gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc
      781 caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg
      841 cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt
      901 gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc
      961 gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag
     1021 ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct
     1081 cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc
     1141 cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct
     1201 ttctagagcc accatgggtg gcgcgcctcc aaaaaagaag agaaaggtag atccaaaaaa
     1261 gaagagaaag gtagatccaa aaaagaagag aaaggtagga ttaattaatg gaagtggaag
     1321 tggaagtgga ggtaccatgt cgcggacccg gctcccttcc ccacccgcac ccagcccagc
     1381 gttttcggcc gactcgttct cagacctgct taggcagttc gacccctcac tgtttaacac
     1441 atcgttgttc gactcccttc ctccgtttgg ggcgcaccat acggaggcgg ccaccgggga
     1501 gtgggatgag gtgcagtcgg gattgagagc tgcggatgca ccacccccaa ccatgcgggt
     1561 ggccgtcacc gctgcccgac cgccgagggc gaagcccgca ccaaggcgga gggcagcgca
     1621 accgtccgac gcaagccccg cagcgcaagt agatttgaga actttgggat attcacagca
     1681 gcagcaggaa aagatcaagc ccaaagtgag gtcgacagtc gcgcagcatc acgaagcgct
     1741 ggtgggtcat gggtttacac atgcccacat cgtagccttg tcgcagcacc ctgcagccct
     1801 tggcacggtc gccgtcaagt accaggacat gattgcggcg ttgccggaag ccacacatga
     1861 ggcgatcgtc ggtgtgggga aacagtggag cggagcccga gcgcttgagg ccctgttgac
     1921 ggtcgcggga gagctgagag ggcctcccct tcagctggac acgggccagt tgctgaagat
     1981 cgcgaagcgg ggaggagtca cggcggtcga ggcggtgcac gcgtggcgca atgcgctcac
     2041 gggagcaccc ctcaacctga ccccagagca ggtcgtggcc attgcctcga atggaggggg
     2101 caaacaggcg ttggaaaccg tacaacgatt gctgccggtg ctgtgccaag cgcacggact
     2161 caccccagag caggtcgtgg cgatcgcaag ccacgacgga ggaaagcaag ccttggaaac
     2221 agtacagagg ctgttgcctg tgctgtgcca agcgcacggc ctcaccccag agcaggtcgt
     2281 ggccattgcc tcgaatggag ggggcaaaca ggcgttggaa accgtacaac gattgctgcc
     2341 ggtgctgtgc caagcgcacg gattaacccc agagcaggtc gtggccattg cctcgaatgg
     2401 agggggcaaa caggcgttgg aaaccgtaca acgattgctg ccggtgctgt gccaagcgca
     2461 cggcttaacc ccagagcagg tcgtggcaat cgcctccaac attggcggga aacaggcact
     2521 cgagactgtc cagcgcctgc ttcccgtgct gtgccaagcg cacggactta ccccagagca
     2581 ggtcgtggcg atcgcaagcc acgacggagg aaagcaagcc ttggaaacag tacagaggct
     2641 gttgcctgtg ctgtgccaag cgcacggcct taccccagag caggtcgtgg ccattgcctc
     2701 gaatggaggg ggcaaacagg cgttggaaac cgtacaacga ttgctgccgg tgctgtgcca
     2761 agcgcacgga ctaaccccag agcaggtcgt ggccattgcc tcgaatggag ggggcaaaca
     2821 ggcgttggaa accgtacaac gattgctgcc ggtgctgtgc caagcgcacg ggctcacccc
     2881 agagcaggtc gtggcaatcg cctccaacat tggcgggaaa caggcactcg agactgtcca
     2941 gcgcctgctt cccgtgctgt gccaagcgca cgggctaacc ccagagcagg tcgtggccat
     3001 tgcctcgaat ggagggggca aacaggcgtt ggaaaccgta caacgattgc tgccggtgct
     3061 gtgccaagcg cacggcctaa ccccagagca ggtcgtggca atcgcctcca acattggcgg
     3121 gaaacaggca ctcgagactg tccagcgcct gcttcccgtg ctgtgccaag cgcacgggtt
     3181 aaccccagag caggtcgtgg caatcgcctc caacattggc gggaaacagg cactcgagac
     3241 tgtccagcgc ctgcttcccg tgctgtgcca agcgcacgga ctcacgcctg agcaggtagt
     3301 ggctattgca tcccatgacg ggggcagacc cgcactggag tcaatcgtgg cccagctttc
     3361 gaggccggac cccgcgctgg ccgcactcac taatgatcat cttgtagcgc tggcctgcct
     3421 cggcggacga cccgccttgg atgcggtgaa gaaggggctc ccgcacgcgc ctgcattgat
     3481 taagcggacc aacagaagga ttcccgagag gacatcacat cgagtggcag atcacgcgca
     3541 agtggtccgc gtgctcggat tcttccagtg tcactcccac cccgcacaag cgttcgatga
     3601 cgccatgact caatttggta tgtcgagaca cggactgctg cagctctttc gtagagtcgg
     3661 tgtcacagaa ctcgaggccc gctcgggcac actgcctccc gcctcccagc ggtgggacag
     3721 gattctccaa gcgagcggta tgaaacgcgc gaagccttca cctacgtcaa ctcagacacc
     3781 tgaccaggcg agccttcatg cgttcgcaga ctcgctggag agggatttgg acgcgccctc
     3841 gcccatgcat gaaggggacc aaactcgcgc gtcagctgga tccgccatca tacacgcggc
     3901 cgcaacaagc ggtccggaag aacttttgag caagaattat catcttgaga acgaagtggc
     3961 tcgtcttaag aaaggttctg gcagtggaga agaactgctt tcaaagaatt accacctgga
     4021 aaatgaggta gctagactga aaaaggggag cggaagtggg gaggagttgc tgagcaaaaa
     4081 ttatcatttg gagaacgaag tagcacgact aaagaaaggg tccggatcgg gtgaggagtt
     4141 actctcgaaa aattatcatc tcgaaaacga agtggctcgg ctaaaaaagg gcagtggttc
     4201 tggagaagag ctattatcta aaaactacca cctcgaaaat gaggtggcac gcttaaaaaa
     4261 gggaagtggc agtggtgaag agctactatc caagaattat catcttgaga acgaggtagc
     4321 gcgtttgaag aagggttccg gctcaggaga ggaactgctc tcgaagaact atcatcttga
     4381 aaatgaggtc gctcgattaa aaaagggatc gggcagtggt gaggaactac tttcaaagaa
     4441 ttaccacctc gaaaacgaag tagctcgatt aaagaaaggt tcagggtcgg gtgaagaatt
     4501 actgagtaaa aattatcatc tggaaaatga ggtagcgaga ctaaaaaagg ggagtggttc
     4561 tggcgaggaa ttgctatcga aaaattatca tcttgagaac gaagttgcta ggctcaaaaa
     4621 gggctcaggc tcaggctgag tttaaacatc gcctgcaggg gaagtggaag tgatatcgga
     4681 agtggaagca ctagtggaag tttcgaagtc gacttgttta ttgcagctta taatggttac
     4741 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     4801 tgtggtttgt ccaaactcat caatgtatct tatgtcgacc ctgtggaatg tgtgtcagtt
     4861 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     4921 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     4981 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     5041 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     5101 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     5161 aggcctaggc ttttgcaaaa agcttaccat ggccaagttg accagtgccg ttccggtgct
     5221 caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg
     5281 ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag
     5341 cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct
     5401 ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg
     5461 gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc
     5521 ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgaccgatcg ataaaataaa
     5581 agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca
     5641 agctcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     5701 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     5761 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     5821 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     5881 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     5941 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     6001 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     6061 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     6121 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     6181 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     6241 gaattcgagc tcggaacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     6301 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     6361 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     6421 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     6481 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     6541 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     6601 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     6661 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     6721 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     6781 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     6841 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     6901 ggcctaggct tttgcgtcga gacgtaccca attcgcccta tagtgagtcg tattacgcgc
     6961 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     7021 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     7081 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcgacgcg ccctgtagcg
     7141 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     7201 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     7261 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     7321 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     7381 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     7441 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     7501 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     7561 aaatattaac gtttacaatt tcccaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     7621 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     7681 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     7741 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     7801 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     7861 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     7921 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     7981 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     8041 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     8101 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     8161 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     8221 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     8281 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     8341 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     8401 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     8461 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     8521 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     8581 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     8641 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     8701 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     8761 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     8821 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     8881 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     8941 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     9001 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     9061 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     9121 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     9181 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     9241 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     9301 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     9361 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     9421 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     9481 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     9541 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     9601 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     9661 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     9721 aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca
     9781 aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg
     9841 gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg
     9901 gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg
     9961 attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga
    10021 tacaataaac gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
    10081 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
    10141 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
    10201 aatctctagc agtggcgccc gaacagggac ctgaaagcga aagggaaacc agagctctct
    10261 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
    10321 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
    10381 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
    10441 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
    10501 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
    10561 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
    10621 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
    10681 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
    10741 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
    10801 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
    10861 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
    10921 cgcagcctca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
    10981 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
    11041 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
    11101 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
    11161 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
    11221 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
    11281 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
    11341 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
    11401 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
    11461 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
    11521 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
    11581 atctcgacgg tatcggttaa cttttaaaag aaaagggggg attggggggt acagtgcagg
    11641 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
    11701 tacaaaaatt caaaatttta tcgatcacga gactagcctc ga
//
