LOCUS       sequence-229330-       10292 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor vector for FLPe recombinase-mediated cassette exchange in 
            master cell lines created with plasmid #112666. This vector allows 
            constitutive expression of your protein of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10292)
  AUTHORS   Ordovas L, Boon R, Pistoni M, Chen Y, Wolfs E, Guo W, Sambathkumar 
            R, Bobis-Wozowicz S, Helsen N, Vanhove J, Berckmans P, Cai Q, 
            Vanuytsel K, Eggermont K, Vanslembrouck V, Schmidt BZ, Raitano S, 
            Van Den Bosch L, Nahmias Y, Cathomen T, Struys T, Verfaillie CM
  TITLE     Efficient Recombinase-Mediated Cassette Exchange in hPSCs to Study 
            the Hepatocyte Lineage Reveals AAVS1 Locus-Mediated Transgene 
            Inhibition.
  JOURNAL   Stem Cell Reports. 2015 Nov 10;5(5):918-931. doi: 
            10.1016/j.stemcr.2015.09.004. Epub 2015 Oct 8.
  PUBMED    26455413
REFERENCE   2  (bases 1 to 10292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2015.09.004"; journalName: "Stem Cell Reports"; 
            date: "2015-11-10- 10"; volume: "5"; issue: "5"; pages: "918-931"
COMMENT     Sequence Label: pZ:F3-P CAGGS tdTPH-F
FEATURES             Location/Qualifiers
     source          1..10292
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..837
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     protein_bind    complement(847..880)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     enhancer        910..1293
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1298..1575
                     /label=chicken beta-actin promoter
     intron          1576..2592
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2516..2538
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2600..2619
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      2686..2695
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2692..4119
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             4141..4197
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4210..5235
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             4210..5235
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    5365..5486
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5402..5421)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5456..5475
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    5596..5707
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     5696..5713
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(5861..6460)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5944..5964)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     6441..6460
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(6470..6523)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    6544..6569
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    complement(6573..6606)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     misc_feature    6613..7416
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(7430..7449)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7431..7449)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7459..7476)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7459..7475)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7468..7490)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7617..8072
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7704..7723)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7914..7935
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8098..8202
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8203..8271,8272..9063)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8421..8440)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9234..9822
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9723..9742
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9976..9993
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10110..10131
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10146..10151,10152..10169,10170..10176)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10184..10200
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10189..10211
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10208..10224
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10208..10224
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10243..10263
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10245..10263
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agagcagagc caggaacccc tgtagggaag gggcaggaga gccaggggca tgagatggtg
       61 gacgaggaag ggggacaggg aagcctgagc gcctctcctg ggcttgccaa ggactcaaac
      121 ccagaagccc agagcagggc cttagggaag cgggaccctg ctctgggcgg aggaatatgt
      181 cccagatagc actggggact ctttaaggaa agaaggatgg agaaagagaa agggagtaga
      241 ggcggccacg acctggtgaa cacctaggac gcaccattct cacaaaggga gttttccaca
      301 cggacacccc cctcctcacc acagccctgc caggacgggg ctggctactg gccttatctc
      361 acaggtaaaa ctgacgcacg gaggaacaat ataaattggg gactagaaag gtgaagagcc
      421 aaagttagaa ctcaggacca acttattctg attttgtttt tccaaactgc ttctcctctt
      481 gggaagtgta aggaagctgc agcaccagga tcagtgaaac gcaccagaca gccgcgtcag
      541 agcagctcag gttctgggag agggtagcgc agggtggcca ctgagaaccg ggcaggtcac
      601 gcatcccccc cttccctccc accccctgcc aagctctccc tcccaggatc ctctctggct
      661 ccatcgtaag caaaccttag aggttctggc aaggagagag atggctccag gaaatggggg
      721 tgtgtcacca gataaggaat ctgcctaaca ggaggtgggg gttagaccca atatcaggag
      781 actaggaagg aggaggccta aggatggggc ttttctgtca ccaatcctgt ccctagtagc
      841 ggccgcgaag ttcctatact ttctagagaa taggaacttc ttcgatctcg agtgtacagc
      901 atcatcgtcg acattgatta ttgactagct agttattaat agtaatcaat tacggggtca
      961 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     1021 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     1081 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     1141 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     1201 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     1261 tacatctacg tattagtcat cgctattacc atgatggtcg aggtgagccc cacgttctgc
     1321 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa
     1381 ttattttgtg cagcgatggg ggcggggggg gggggggggc gcgcgccagg cggggcgggg
     1441 cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc
     1501 gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga
     1561 agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc
     1621 gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg
     1681 gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt
     1741 ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc
     1801 tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc
     1861 ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag
     1921 gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc
     1981 tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg
     2041 caaccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg
     2101 ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg
     2161 gtgccgggcg gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc
     2221 ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa
     2281 tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg
     2341 aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag
     2401 gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag
     2461 cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc
     2521 ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc
     2581 tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa
     2641 gaattcattt aaatatgcat acgtatacca attgatatca ccggtgccac catggtgagc
     2701 aagggcgagg aggtcatcaa agagttcatg cgcttcaagg tgcgcatgga gggctccatg
     2761 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     2821 accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc
     2881 ccccagttca tgtacggctc caaggcgtac gtgaagcacc ccgccgacat ccccgattac
     2941 aagaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     3001 ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg
     3061 aagatgcgcg gcaccaactt cccccccgac ggccccgtaa tgcagaagaa gaccatgggc
     3121 tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac
     3181 caggccctga agctgaagga cggcggccac tacctggtgg agttcaagac catctacatg
     3241 gccaagaagc ccgtgcaact gcccggctac tactacgtgg acaccaagct ggacatcacc
     3301 tcccacaacg aggactacac catcgtggaa cagtacgagc gctccgaggg ccgccaccac
     3361 ctgttcctgg ggcatggcac cggcagcacc ggcagcggca gctccggcac cgcctcctcc
     3421 gaggacaaca acatggccgt catcaaagag ttcatgcgct tcaaggtgcg catggagggc
     3481 tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     3541 acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc
     3601 ctgtcccccc agttcatgta cggctccaag gcgtacgtga agcaccccgc cgacatcccc
     3661 gattacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     3721 gacggcggtc tggtgaccgt gacccaggac tcctccctgc aggacggcac gctgatctac
     3781 aaggtgaaga tgcgcggcac caacttcccc cccgacggcc ccgtaatgca gaagaagacc
     3841 atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag
     3901 atccaccagg ccctgaagct gaaggacggc ggccactacc tggtggagtt caagaccatc
     3961 tacatggcca agaagcccgt gcaactgccc ggctactact acgtggacac caagctggac
     4021 atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgctc cgagggccgc
     4081 caccacctgt tcctgtacgg catggacgag ctgtacaagg gtggtggctc ggggggagga
     4141 gcgacaaact ttagcttgct gaagcaagct ggtgacgttg aggagaatcc cggaccagga
     4201 ttcacgcgta tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa
     4261 aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc
     4321 agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc
     4381 tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg
     4441 cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt
     4501 gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag
     4561 gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga
     4621 ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc
     4681 catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct
     4741 ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg
     4801 gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg
     4861 agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg
     4921 tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca
     4981 ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc
     5041 ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc
     5101 cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg
     5161 accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg
     5221 agggcaaagg aatagcacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg
     5281 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     5341 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     5401 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     5461 ttgtccaaac tcatcaatgt atcttatcat gtctgttcga actagtgtcg agtgctattg
     5521 tcttcccaat cctccccctt gctgtcctgc cccaccccac cccccagaat agaatgacac
     5581 ctactcagac aatgcgatgc aatttcctca ttttattagg aaaggacagt gggagtggca
     5641 ccttccaggg tcaaggaagg cacgggggag gggcaaacaa cagatggctg gcaactagaa
     5701 ggcacagtcg aggctgatca gcgggtttaa acgggccctc tagagtcggt gggcctcggg
     5761 ggcgggtgcg gggtcggcgg ggccgccccg ggtggcttcg gtcggagcca tggggtcgtg
     5821 cgctcctttc ggtcgggcgc tgcgggtcgt ggggcgggcg tcaggcaccg ggcttgcggg
     5881 tcatgcacca ggtgcgcggt ccttcgggca cctcgacgtc ggcggtgacg gtgaagccga
     5941 gccgctcgta gaaggggagg ttgcggggcg cggaggtctc caggaaggcg ggcaccccgg
     6001 cgcgctcggc cgcctccact ccggggagca cgacggcgct gcccagaccc ttgccctggt
     6061 ggtcgggcga gacgccgacg gtggccagga accacgcggg ctccttgggc cggtgcggcg
     6121 ccaggaggcc ttccatctgt tgctgcgcgg ccagccggga accgctcaac tcggccatgc
     6181 gcgggccgat ctcggcgaac accgcccccg cttcgacgct ctccggcgtg gtccagaccg
     6241 ccaccgcggc gccgtcgtcc gcgacccaca ccttgccgat gtcgagcccg acgcgcgtga
     6301 ggaagagttc ttgcagctcg gtgacccgct cgatgtggcg gtccgggtcg acggtgtggc
     6361 gcgtggcggg gtagtcggcg aacgcggcgg cgagggtgcg tacggcccgg gggacgtcgt
     6421 cgcgggtggc gaggcgcacc gtgggcttgt actcggtcat ttcgagccta gggccgggat
     6481 tctcctccac gtcaccgcat gttagaagac ttcctctgcc ctctccgctg ccagatctga
     6541 ggccctgtgg gaggaagaga agaggtcagg ccgaagttcc tatactattt gaagaatagg
     6601 aacttcaagc ttgccccact gtggggtgga ggggacagat aaaagtaccc agaaccagag
     6661 ccacattaac cggccctggg aatataaggt ggtcccagct cggggacaca ggatccctgg
     6721 aggcagcaaa catgctgtcc tgaagtggac ataggggccc gggttggagg aagaagacta
     6781 gctgagctct cggacccctg gaagatgcca tgacaggggg ctggaagagc tagcacagac
     6841 tagagaggta aggggggtag gggagctgcc caaatgaaag gagtgagagg tgacccgaat
     6901 ccacaggaga acggggtgtc caggcaaaga aagcaagagg atggagaggt ggctaaagcc
     6961 agggagacgg ggtactttgg ggttgtccag aaaaacggtg atgatgcagg cctacaagaa
     7021 ggggaggcgg gacgcaaggg agacatccgt cggagaaggc catcctaaga aacgagagat
     7081 ggcacaggcc ccagaaggag aaggaaaagg gaacccagcg agtgaagacg gcatggggtt
     7141 gggtgaggga ggagagatgc ccggagagga cccagacacg gggaggatcc gctcagagga
     7201 catcacgtgg tgcagcgccg agaaggaagt gctccggaaa gagcatcctt gggcagcaac
     7261 acagcagaga gcaaggggaa gagggagtgg aggaagacgg aacctgaagg aggcggcagg
     7321 gaaggatctg ggccagccgt agaggtgacc caggccacaa gctgcagaca gaaagcggca
     7381 caggcccagg ggagagaatg ctggtcagag aaagcaggta cccaattcgc cctatagtga
     7441 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     7501 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     7561 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     7621 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     7681 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     7741 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     7801 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     7861 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     7921 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     7981 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     8041 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     8101 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     8161 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     8221 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     8281 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     8341 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     8401 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     8461 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     8521 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     8581 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     8641 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     8701 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     8761 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     8821 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     8881 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     8941 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     9001 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     9061 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     9121 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     9181 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     9241 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     9301 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     9361 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     9421 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     9481 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     9541 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     9601 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     9661 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     9721 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     9781 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     9841 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     9901 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     9961 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
    10021 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
    10081 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
    10141 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
    10201 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
    10261 agggaacaaa agctggagct ccaccgcggt gg
//
