LOCUS       sequence-229334-        2787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a Flag-tagged version of DDX11 KAK mutant in mammalian
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Cortone G, Zheng G, Pensieri P, Chiappetta V, Tate R, Malacaria E, 
            Pichierri P, Yu H, Pisani FM
  TITLE     Interaction of the Warsaw breakage syndrome DNA helicase DDX11 with 
            the replication fork-protection factor Timeless promotes sister 
            chromatid cohesion.
  JOURNAL   PLoS Genet. 2018 Oct 10;14(10):e1007622. doi: 
            10.1371/journal.pgen.1007622. eCollection 2018 Oct.
  PUBMED    30303954
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2018-10-10"; volume: "14(10):e1007622. doi"; pages: 
            " 10.1371/journal.pgen.1007622. eCollection 2018 Oct"
COMMENT     Sequence Label: pcDNA-DDX11-Flag-KAK
FEATURES             Location/Qualifiers
     source          1..2787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    649..660
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     CDS             2719..2784
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 atggctaatg aaacacagaa ggttggtgcc atccattttc cttttccctt cacaccctat
       61 tccatccagg aagacttcat ggcagagctg taccgggttt tggaggctgg caagattggg
      121 atatttgaga gtccaactgg cactgggaag tccttaagtc ttatttgtgg ggccctctct
      181 tggctccgtg actttgaaca gaagaagcgt gaagaagagg cacgactcct tgaaactgga
      241 actggcccct tacatgatga gaaagatgaa tccctgtgtc tgtcttcttc ctgcgaaggg
      301 gctgcaggca ccccgaggcc tgctggagaa ccggcctggg ttactcagtt tgtgcagaag
      361 aaagaagaga gggacctggt ggaccgacta aaggcggagc aggccaggag gaagcagcga
      421 gaagaacgcc tgcagcagct gcagcacagg gtgcagctca agtatgcagc caagcgcctg
      481 aggcaggaag aagaagaaag agagaatctc ctccgcctca gcagggagat gctagagaca
      541 ggcccggagg ctgagcggct ggagcagctg gagtctgggg aggaggagct ggtcctcgcc
      601 aaagccaaga gtgatgagga gaaaaaggtg gcgagcagag tggatgagga tgaggatgac
      661 ctggaggaag aacacataac taagatttat tactgtagtc ggacacactc ccagctggcc
      721 cagtttgtgc atgaggtgaa gaagagcccc tttggcaagg atgttcggct ggtctccctt
      781 ggctcccggc agaacctttg tgtaaatgaa gacgtgaaaa gcctaggttc tgtgcagctt
      841 atcaacgacc gctgtgtgga catgcagaga agcaggcacg agaagaagaa aggagctgag
      901 gaggagaagc caaagaggag gaggcaggag aagcaggcag cctgcccctt ctacaaccac
      961 gagcagatgg gccttctccg ggatgaggcc ctggcagagg tgaaggacat ggagcagctg
     1021 ctggcccttg ggaaggaggc ccgggcctgt ccctattacg ggagccgcct tgccatccct
     1081 gcagcccagc tggtggtgct gccctatcag atgctgctgc atgcggccac tcggcaggcc
     1141 gcgggcatcc ggctgcagga ccaggtggtg atcatcgacg aggcgcacaa cctgatcgac
     1201 accatcacgg gcatgcacag cgtggaggtc agcggctccc agctctgcca ggcccattcc
     1261 cagctgctgc agtacgtgga gcgatacggg aagcgtttga aggccaagaa cctgatgtac
     1321 ctgaagcaga tcctgtattt gctggagaaa ttcgtggctg tgctaggggg gaacattaag
     1381 caaaatccca atacacagag tctgtcacag acagggacgg agctgaagac catcaacgac
     1441 tttctcttcc agagccagat cgacaacatc aacctgttca aggtgcagcg atactgtgag
     1501 aagagcatga tcagcagaaa gctctttgga ttcactgaac ggtacggagc agtgttctca
     1561 tcccgggagc agcccaaact ggctgggttt cagcaattcc tgcagagcct gcagcccagg
     1621 acgactgaag cacttgcagc ccctgcagac gagagtcagg ccagcaccct gcgaccagct
     1681 tctccactga tgcacattga aggcttcctg gcagctctca ctacggccaa ccaggacggc
     1741 agggtcatcc tgagccgcca aggcagcctc agtcagagca ccctgaagtt tttgctcctg
     1801 aatccagctg tgcactttgc ccaagtggtg aaggaatgcc gggcagtggt cattgcgggg
     1861 ggtaccatgc agccggtgtc tgacttccgg cagcagctgc tggcctgtgc cggggtggaa
     1921 gctgagcgcg tggtggagtt ttcctgtggt cacgtgatcc ctccagacaa catcctgccc
     1981 cttgtcatct gcagcgggat ctccaaccag ccgctggaat tcacgttcca gaaaagagag
     2041 ctgcctcaga tgatggacga ggtgggtcgc attctctgta acctgtgcgg tgtggttcct
     2101 ggaggggtgg tctgtttctt cccctcctac gagtacctgc gccaggtcca tgcccactgg
     2161 gagaagggtg gcctgctggg ccgtctggct gccaggaaga agatattcca ggaacctaag
     2221 agcgcacacc aggtggagca ggtgctgctg gcatattcca ggtgcatcca ggcctgtggc
     2281 caggagagag gccaggtgac aggggccctg ctcctctctg tggttggagg aaagatgagt
     2341 gaagggatca acttctctga caacctaggc cggtgtgtgg tgatggtggg catgcccttc
     2401 cccaacatca ggtctgcaga gctgcaggag aagatggcct acttggatca aaccctcccc
     2461 agagcccccg gccaggcacc cccagggaag gctctggtgg agaacctgtg catgaaggcc
     2521 gtcaaccagt ccataggcag ggccatcagg caccagaagg attttgccag cgtagtgctc
     2581 ctggaccagc gatatgcccg gccccctgtc ctggccaagc tgccggcctg gatccgagcc
     2641 cgtgtggagg tcaaagctac ctttggcccc gccattgctg ctgtgcagaa gtttcaccgg
     2701 gagaagtcgg cctcttccga ctacaaagac catgacggtg attataaaga tcatgacatc
     2761 gattacaagg atgacgatga caagtga
//
