LOCUS       sequence-229339-        9663 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual luciferase assay for miRNA-targeted UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9663)
  AUTHORS   Kutsche LK, Gysi DM, Fallmann J, Lenk K, Petri R, Swiersy A, Klapper
            SD, Pircs K, Khattak S, Stadler PF, Jakobsson J, Nowick K, Busskamp 
            V
  TITLE     Combined Experimental and System-Level Analyses Reveal the Complex 
            Regulatory Network of miR-124 during Human Neurogenesis.
  JOURNAL   Cell Syst. 2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011.
  PUBMED    30292704
REFERENCE   2  (bases 1 to 9663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011."
COMMENT     Sequence Label: pmiRGLO-CD164-201
FEATURES             Location/Qualifiers
     source          1..9663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    67..350
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
     promoter        407..906
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(442..465)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     839..858
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      947..956
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             953..2605
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    5086..5207
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5097..5116)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5151..5170
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        5452..5809
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5452..5643
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5475..5495)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5660..5795
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      5854..5863
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5860..6792
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             6844..7635
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6895..6914)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7505..7524
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7699..7747
                     /note="synthetic polyadenylation signal"
     promoter        7903..8007
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8008..8076,8077..8868)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8226..8245)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(9026..9614)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9106..9125)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
       61 tatagtccgg aaatacagga acgcacgctg gatggccctt cgctgggatg gtgaaaccat
      121 gaaaaatggc agcttcagtg gattaagtgg gggtaatgtg gcctgtaccc tctggttgca
      181 taggtattca tacggttaaa atttatcagg cgcgattgcg gcagtttttc gggtggtttg
      241 ttgccatttt tacctgtctg ctgccgtgat cgcgctgaac gcgttttagc ggtgcgtaca
      301 attaagggat tatggtaaat ccacttactg tctgccctcg tagccatcga gataaaccgc
      361 agtactccgg ccacgatgcg tccggcgtag aggatcgaga tctaccgggt aggggaggcg
      421 cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac
      481 acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg
      541 ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc
      601 gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc
      661 gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa
      721 tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc
      781 gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca
      841 ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc
      901 ctttcgacct gcagcccaag cttggcaatc cggtactgtt ggtaaagcca ccatggaaga
      961 tgccaaaaac attaagaagg gcccagcgcc attctaccca ctcgaagacg ggaccgccgg
     1021 cgagcagctg cacaaagcca tgaagcgcta cgccctggtg cccggcacca tcgcctttac
     1081 cgacgcacat atcgaggtgg acattaccta cgccgagtac ttcgagatga gcgttcggct
     1141 ggcagaagct atgaagcgct atgggctgaa tacaaaccat cggatcgtgg tgtgcagcga
     1201 gaatagcttg cagttcttca tgcccgtgtt gggtgccctg ttcatcggtg tggctgtggc
     1261 cccagctaac gacatctaca acgagcgcga gctgctgaac agcatgggca tcagccagcc
     1321 caccgtcgta ttcgtgagca agaaagggct gcaaaagatc ctcaacgtgc aaaagaagct
     1381 accgatcata caaaagatca tcatcatgga tagcaagacc gactaccagg gcttccaaag
     1441 catgtacacc ttcgtgactt cccatttgcc acccggcttc aacgagtacg acttcgtgcc
     1501 cgagagcttc gaccgggaca aaaccatcgc cctgatcatg aacagtagtg gcagtaccgg
     1561 attgcccaag ggcgtagccc taccgcaccg caccgcttgt gtccgattca gtcatgcccg
     1621 cgaccccatc ttcggcaacc agatcatccc cgacaccgct atcctcagcg tggtgccatt
     1681 tcaccacggc ttcggcatgt tcaccacgct gggctacttg atctgcggct ttcgggtcgt
     1741 gctcatgtac cgcttcgagg aggagctatt cttgcgcagc ttgcaagact ataagattca
     1801 atctgccctg ctggtgccca cactatttag cttcttcgct aagagcactc tcatcgacaa
     1861 gtacgaccta agcaacttgc acgagatcgc cagcggcggg gcgccgctca gcaaggaggt
     1921 aggtgaggcc gtggccaaac gcttccacct accaggcatc cgccagggct acggcctgac
     1981 agaaacaacc agcgccattc tgatcacccc cgaaggggac gacaagcctg gcgcagtagg
     2041 caaggtggtg cccttcttcg aggctaaggt ggtggacttg gacaccggta agacactggg
     2101 tgtgaaccag cgcggcgagc tgtgcgtccg tggccccatg atcatgagcg gctacgttaa
     2161 caaccccgag gctacaaacg ctctcatcga caaggacggc tggctgcaca gcggcgacat
     2221 cgcctactgg gacgaggacg agcacttctt catcgtggac cggctgaaga gcctgatcaa
     2281 atacaagggc taccaggtag ccccagccga actggagagc atcctgctgc aacaccccaa
     2341 catcttcgac gccggggtcg ccggcctgcc cgacgacgat gccggcgagc tgcccgccgc
     2401 agtcgtcgtg ctggaacacg gtaaaaccat gaccgagaag gagatcgtgg actatgtggc
     2461 cagccaggtt acaaccgcca agaagctgcg cggtggtgtt gtgttcgtgg acgaggtgcc
     2521 taaaggactg accggcaagt tggacgcccg caagatccgc gagattctca ttaaggccaa
     2581 gaagggcggc aagatcgccg tgtaattcta gttgtttaaa cgagacagac ccattgaatt
     2641 aataaggact ggtgattcat ttgtgtaact cactgaagcc aaaatactat cttttaagat
     2701 gtcccacatg gaagacgcta ttccaggatc tttaaatttc catggatgca tataggatgt
     2761 ttgggagcat catccgtgaa gaaaaaatca attaaatcat tgtgttcaac aggaatattt
     2821 aaaatattct gcatgaatcc tgtggctgtc ttattttaaa tagctgctgc tgtgggatta
     2881 tatttttttt ccttaacatg ccaaatataa ctttctgaaa gtgatggaaa atgttgtctt
     2941 gtgcagacaa catcatggct cttggcagtt taaatttagt aattttaatt tagtgaacag
     3001 aattgagaag aacgtgccaa atgagaatca attaggtgga tttttggctg tcatttcaaa
     3061 agtggaataa atttattaat ttagtagtac taaatggtat ccttagatta aaattttgtg
     3121 cttgataaca gctgtttttt ctacattaga aataagatgc cacacaagga actacattcc
     3181 agatttaaag aaatgaaagg ataccattag tgtgtataac agattattgt tcatacttgt
     3241 aaagcatctt atgtcattga gaatataaag aacagtgcct tagaagacag tgaaaggtaa
     3301 gctctagctt aatgtctatg atttgttctt tgacattaag gaaggtaagg attggtcaga
     3361 ggatgtaact tgatgtgagc agtagtaaac ctgttttaga tatcatactg ttaatatttt
     3421 attgaaaatt tatttcagag cggagaaact taagctaaag tctgttatac agaattgaaa
     3481 gccttcgtat cttgaacctc ccaacatttt tcttatggct gttgaaaagt atagagctaa
     3541 attgatttaa ttacactttc ctttgtactt taaaaaaaag tatgctagca ctattgtacc
     3601 ttgaaaggat ttccaccaga ctgtcttgag tagtgacttc tttggtgagg caagaaggat
     3661 atacattatt ttagaatcat ttactattta aatgagacaa tcatattatt ttagaatcat
     3721 ttattttaaa tgagacaatc attttaagtt ttaagataac agaagtgacc aatgtaattt
     3781 cacaacacct aaggattttt tggttgatca ggttactgta gatttttact gattgtcctg
     3841 gatgaataga ctgtgctttt tctttttctc tcccttcctt cttggtttcc catagtataa
     3901 taagcatgca tactttaact tctatagttt tctcctttag agggtcgtct tcagttttag
     3961 aggtttactt ctcccttgcc tttgactcat tggactagtg cagaggcttt aagtagttta
     4021 aaatgggctt ttgcttttct aggtcattaa cgttttttat ttagtttctt tagccaatag
     4081 tgactgagtt tcgcacttga ttttcaatat tttatagtaa gaaatgacaa actgctttgt
     4141 ttcatttcat aaacaaactc tgcatttaga taactattaa aggttgttaa gatgaagatt
     4201 tactgtttct ttgttactcg ttggtacagc tgtttgtttt acttgcacat ttgtacatat
     4261 acttaatgtt ttcaagtgcc ttaattgttt aaaatctctg gcttcaaagt ttcttgggga
     4321 aaggtcggtt tacctcacat tttttgtttc cattagtaat attctaggta cctcacaaaa
     4381 tgtattatgg tgccatggct gttagttttt agtgagtgct gtaggattaa ttcgaaaata
     4441 ggcagaattc cattcctccc aaggtggcaa aaattagcta tactgatgta attgtcattt
     4501 acctgggtat gaattccctg acacacattc atgtcaacat atgtagcaaa ttttgtgaaa
     4561 acataacaat ttgaagcttc tgtaattttg agcactgctc taacaacaag cataatataa
     4621 aattagttag attttgcaag tctacaaatg agctcttgca acagaactca cagccttttt
     4681 acttttttcc cctaacttta gcaatgtagt atcttgagcc attaattttt gggttttttt
     4741 aaaatccaga aggtatatag aaaccttttc agatttttca tctgatttgt tcttgcagat
     4801 gttcttctat caaatacctt attttacctt acagatattt gttgcacagg cagatactgc
     4861 tgtatttaga catttctatt tcagttcatt aaaaactgca aaaccaatct gtatcatgta
     4921 ccaaactgac ttaaaataaa tctacatgtt tattgaaagt cgacctgcag gcatgcaagc
     4981 tgatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
     5041 ataactagca taaccccttg gggcggccgc ttcgagcaga catgataaga tacattgatg
     5101 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     5161 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     5221 gcattcattt tatgtttcag gttcaggggg agatgtggga ggttttttta agcaagtaaa
     5281 acctctacaa atgtggtaaa atcgaatttt aacaaaatat taacgcttac aatttcctga
     5341 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgc ggatctgcgc
     5401 agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct tctgaggcgg
     5461 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     5521 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     5581 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     5641 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     5701 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     5761 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttgattc
     5821 ttctgacaca acagtctcga accaaaggct ggagccacca tggcttccaa ggtgtacgac
     5881 cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg caagcaaatg
     5941 aacgtgctgg actccttcat caactactat gattccgaga agcacgccga gaacgccgtg
     6001 atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc
     6061 gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc cggcaagagc
     6121 gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg gttcgagctg
     6181 ctgaaccttc caaagaaaat catctttgtg ggccacgact ggggggcttg tctggccttt
     6241 cactactcct acgagcacca agacaagatc aaggccatcg tccatgctga gagtgtcgtg
     6301 gacgtgatcg agtcctggga cgagtggcct gacatcgagg aggatatcgc cctgatcaag
     6361 agcgaagagg gcgagaaaat ggtgcttgag aataacttct tcgtcgagac catgctccca
     6421 agcaagatca tgcggaaact ggagcctgag gagttcgctg cctacctgga gccattcaag
     6481 gagaagggcg aggttagacg gcctaccctc tcctggcctc gcgagatccc tctcgttaag
     6541 ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca acgcctacct tcgggccagc
     6601 gacgatctgc ctaagatgtt catcgagtcc gaccctgggt tcttttccaa cgctattgtc
     6661 gagggagcta agaagttccc taacaccgag ttcgtgaagg tgaagggcct ccacttcagc
     6721 caggaggacg ctccagatga aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg
     6781 aagaacgagc agaccggtgg tgggagcgga ggtggcggat caggtggcgg aggctccgga
     6841 gggattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     6901 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     6961 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     7021 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     7081 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     7141 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     7201 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     7261 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     7321 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     7381 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     7441 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     7501 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     7561 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     7621 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     7681 tgccatcacg atggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt
     7741 ttgtgtgaat cgatagcgat aaggatcctc tttgcgcttg cgttttccct tgtccagata
     7801 gcccagtagc tgacattcat ccggggtcag caccgtttct gcggactggc tttctacgta
     7861 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     7921 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     7981 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     8041 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     8101 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     8161 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cactttcaaa
     8221 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     8281 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     8341 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     8401 gcggccaact tacttctgac aactatcgga ggaccgaagg agctaaccgc ttttttgcac
     8461 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     8521 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     8581 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     8641 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     8701 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     8761 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     8821 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaatt cgaaatgacc
     8881 gaccaagcga cgcccaaccg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     8941 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     9001 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     9061 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     9121 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     9181 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     9241 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     9301 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     9361 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     9421 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     9481 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     9541 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     9601 aaaaaggatt tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     9661 aaa
//
