LOCUS       sequence-229342-        1311 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Part of GoldenGate Yarrowia lipolytica toolkit for assembling an 
            expression plasmid for Y. lipolytic yeast. Auxotrophy marker for 
            selection after transformation in Y. lipolytica (Marker_URA3).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Larroude M, Park YK, Soudier P, Kubiak M, Nicaud JM, Rossignol T
  TITLE     A modular Golden Gate toolkit for Yarrowia lipolytica synthetic 
            biology.
  JOURNAL   Microb Biotechnol. 2019 May 31. doi: 10.1111/1751-7915.13427.
  PUBMED    31148366
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb 
            Biotechnol. 2019 May 31. doi: 10.1111/1751-7915.13427."
COMMENT     Sequence Label: GGE083
FEATURES             Location/Qualifiers
     source          1..1311
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(12..45)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ggtctctagg tataacttcg tatagcatac attatacgaa gttattctga attccgagaa
       61 acacaacaac atgccccatt ggacagacca tgcggataca caggttgtgc agtaccatac
      121 atactcgatc agacaggtcg tctgaccatc atacaagctg aacagcgctc catacttgca
      181 cgctctctat atacacagtt aaattacata tccatagtct aacctctaac agttaatctt
      241 ctggtaagcc tcccagccag ccttctggta tcgcttggcc tcctcaatag gatctcggtt
      301 ctggccgtac agacctcggc cgacaattat gatatccgtt ccggtagaca tgacatcctc
      361 aacagttcgg tactgctgtc cgagagcgtc tcccttgtcg tcaagaccca ccccgggggt
      421 cagaataagc cagtcctcag agtcgccctt aggtcggttc tgggcaatga agccaaccac
      481 aaactcgggg tcggatcggg caagctcaat ggtctgcttg gagtactcgc cagtggccag
      541 agagcccttg caagacagct cggccagcat gagcagacct ctggccagct tctcgttggg
      601 agaggggact aggaactcct tgtactggga gttctcgtag tcagagacgt cctccttctt
      661 ctgttcagag acagtttcct cggcaccagc tcgcaggcca gcaatgattc cggttccggg
      721 tacaccgtgg gcgttggtga tatcggacca ctcggcgatt cggtgacacc ggtactggtg
      781 cttgacagtg ttgccaatat ctgcgaactt tctgtcctcg aacaggaaga aaccgtgctt
      841 aagagcaagt tccttgaggg ggagcacagt gccggcgtag gtgaagtcgt caatgatgtc
      901 gatatgggtc ttgatcatgc acacataagg tccgacctta tcggcaagct caatgagctc
      961 cttggtggtg gtaacatcca gagaagcaca caggttggtt ttcttggctg ccacgagctt
     1021 gagcactcga gcggcaaagg cggacttgtg gacgttagct cgagcttcgt aggagggcat
     1081 tttggtggtg aagaggagac tgaaataaat ttagtctgca gaacttttta tcggaacctt
     1141 atctggggca gtgaagtata tgttatggta atagttacga gttagttgaa cttatagata
     1201 gactggacta tacggctatc ggtccaaatt agaaagaacg tcaatggctc tctgggcgcg
     1261 aattcgtata acttcgtata gcaggagtta tccgaagcga acggagagac c
//
