LOCUS       sequence-229344-        1900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Part of GoldenGate Yarrowia lipolytica toolkit for assembling an 
            expression plasmid for Y. lipolytic yeast. Auxotrophy marker for 
            selection after transformation in Y. lipolytica (Marker_Leu2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Larroude M, Park YK, Soudier P, Kubiak M, Nicaud JM, Rossignol T
  TITLE     A modular Golden Gate toolkit for Yarrowia lipolytica synthetic 
            biology.
  JOURNAL   Microb Biotechnol. 2019 May 31. doi: 10.1111/1751-7915.13427.
  PUBMED    31148366
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb 
            Biotechnol. 2019 May 31. doi: 10.1111/1751-7915.13427."
COMMENT     Sequence Label: GGE105
FEATURES             Location/Qualifiers
     source          1..1900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(31..64)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ggtctctagg tattaccctg ttatccctac ataacttcgt atagcataca ttatacgaag
       61 ttattctgaa ttccgcctga gtcatcattt atttaccagt tggccacaaa cccttgacga
      121 tctcgtatgt cccctccgac atactcccgg ccggctgggt acgttcgata gcgctatcgg
      181 catcgacaag gtttgggtcc ctagccgata ccgcactacc tgagtcacaa tcttcggagg
      241 tttagtcttc cacatagcac gggcaaaagt gcgtatatat acaagagcgt ttgccagcca
      301 cagattttca ctccacacac cacatcacac atacaaccac acacatccac aatggaaccc
      361 gaaactaaga agaccaagac tgactccaag aagattgttc ttctcggcgg cgacttctgt
      421 ggccccgagg tgattgccga ggccgtcaag gtgctcaagt ctgttgctga ggcctccggc
      481 accgagtttg tgttcgagga ccgactcatt ggaggagctg ccattgagaa ggagggcgag
      541 cccatcaccg acgctactct cgacatctgc cgaaaggctg actctattat gctcggtgct
      601 gtcggaggcg ctgccaacac cgtatggacc actcccgacg gacgaaccga cgtgcgaccc
      661 gagcagggcc tcctcaagct gcgaaaggac ctgaacctgt acgccaacct gcgaccctgc
      721 cagctgctgt cgcccaagct cgccgatctc tcccccatcc gaaacgttga gggcaccgac
      781 ttcatcattg tccgagagct cgtcggaggt atctactttg gagagcgaaa ggaggatgac
      841 ggatctggcg tcgcttccga caccgagact tactccgttc ctgaggttga gcgaattgcc
      901 cgaatggccg ccttcctggc ccttcagcac aacccccctc ttcccgtgtg gtcccttgac
      961 aaggccaacg tgctggcctc ctctcgactt tggcgaaaga ctgtcacccg agtcctcaag
     1021 gacgagttcc cccagctgga gctcaaccac cagctgatcg actcggccgc catgatcctc
     1081 atcaagcagc cctccaagat gaatggtatc atcatcacca ccaacatgtt tggcgatatc
     1141 atctccgacg aggcctccgt catccccggt tctctgggtc tgctgccctc cgcctctctg
     1201 gcttctctgc ccgacaccaa cgaggcgttc ggtctgtacg agccctgtca cggatctgcc
     1261 cccgatctcg gcaagcagaa ggtcaacccc attgccacca ttctgtctgc cgccatgatg
     1321 ctcaagttct ctcttaacat gaagcccgcc ggtgacgctg ttgaggctgc cgtcaaggag
     1381 tccgtcgagg ctggtatcac taccgccgat atcggaggct cttcctccac ctccgaggtc
     1441 ggagactttg ttgccaacaa ggtcaaggag ctgctcaaga aggagtaagt cgtttctacg
     1501 acgcattgat ggaaggagca aactgacgcg cctgcgggtt ggtctaccgg cagcagccct
     1561 gctaatgaaa tgatgattta taatttaccg gtgtagcaac cttgactaga agaagcagat
     1621 tgggtgtgtt tgtagtggag gacagtggta cgttttggaa acagtcttct tgaaagtgtc
     1681 ttgtctacag tatattcact cataacctca atagccaagg gtgtagtcgg tttattaaag
     1741 gaagggagtt gtggctgatg tggatagata tctttaaagc tggcgactgc acccaacgag
     1801 tgtggtggta gcttgttact gtatattcga attcgtataa cttcgtatag caggagttat
     1861 ccgaagcgat aattaccctg ttatccctaa cggagagacc
//
