LOCUS       sequence-229346-        1642 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Part of GoldenGate Yarrowia lipolytica toolkit for assembling an 
            expression plasmid for Y. lipolytic yeast. Antibiotic resistance 
            marker for selection after transformation in Y. lipolytica 
            (Marker_hph).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Larroude M, Park YK, Soudier P, Kubiak M, Nicaud JM, Rossignol T
  TITLE     A modular Golden Gate toolkit for Yarrowia lipolytica synthetic 
            biology.
  JOURNAL   Microb Biotechnol. 2019 May 31. doi: 10.1111/1751-7915.13427.
  PUBMED    31148366
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb 
            Biotechnol. 2019 May 31. doi: 10.1111/1751-7915.13427."
COMMENT     Sequence Label: GGE367
FEATURES             Location/Qualifiers
     source          1..1642
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(31..64)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             429..1454
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
ORIGIN
        1 ggtctctagg ttagggataa cagggtaatg ataacttcgt atagcataca ttatacgaag
       61 ttatgggttg gcggcgcatt tgtgtcccaa aaaacagccc caattgcccc aattgacccc
      121 aaattgaccc agtagcggac ccaaccccgg cgagagcccc cttcacccca catatcaaac
      181 ctcccccggt tcccacactt gccgttaagg gcgtagggta ctgcagtctg gaatctacgc
      241 ttgttcagac tttgtactag tttctttgtc tggccatccg ggtaacccat gccggacgca
      301 aaatagacta ctgaaaattt ttttgctttg tggttgggac tttagccaag ggtataaaag
      361 accaccgtcc ccgaattacc tttcctcttc ttttctctct ctccttgtca actcacaccc
      421 gaagcacaat gaagaagccc gagctgaccg ctacctctgt tgagaagttc ctgattgaga
      481 agtttgattc cgtttccgac ctgatgcagc tgtccgaggg cgaggagtct cgagccttct
      541 cctttgacgt gggcggacga ggttacgttc tgcgagtgaa ctcgtgtgcc gacggcttct
      601 acaaggatcg atacgtctac cgacactttg cttctgccgc tctgcccatc cctgaggttc
      661 tcgacattgg cgagttctct gagtccctca cctactgcat ctctcgacga gctcagggag
      721 tcaccctgca ggacctccct gagactgagc tgcctgctgt cctccagcct gttgctgagg
      781 ccatggacgc tatcgctgct gctgatctgt cccagacctc gggtttcggc ccctttggac
      841 ctcagggaat tggacagtac accacttggc gagacttcat ctgtgctatt gccgatcctc
      901 acgtctacca ttggcagacc gttatggacg atactgtgtc ggcttctgtc gctcaggctc
      961 tggacgagct gatgctctgg gccgaggatt gccccgaggt tcgacacctg gtgcatgctg
     1021 acttcggttc caacaacgtt ctcaccgaca acggccgaat cactgccgtg attgactggt
     1081 ccgaggctat gtttggcgac tcgcagtacg aggtggccaa catcttcttt tggcgaccct
     1141 ggctggcttg tatggagcag cagacccgat acttcgagcg acgacatcct gagctcgctg
     1201 gctcccctcg actgcgagct tacatgctcc gaattggtct ggaccagctc taccagtcgc
     1261 tggtggatgg caactttgac gatgctgcct gggctcaggg acgatgtgac gccatcgtgc
     1321 gatctggcgc tggaaccgtc ggacgaactc agattgcccg acgatccgct gctgtctgga
     1381 ccgacggatg cgtggaggtc ctggctgatt cgggtaaccg acgaccctct actcgacctc
     1441 gagctaagga gtaagtgtct gtggtatcta agctatttat cactctttac aacttctacc
     1501 tcaactatct actttaataa atgaatatcg tttattctct atgattactg tatatgcgtt
     1561 cctctaagac aaatcgaata taacttcgta tagcaggagt tatccgaagc gagtagggat
     1621 aacagggtaa tacggagaga cc
//
