LOCUS       sequence-22935-e         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Du SJ, Devoto SH, Westerfield M, Moon RT
  TITLE     Positive and negative regulation of muscle cell identity by members 
            of the hedgehog and TGF-beta gene families.
  JOURNAL   J Cell Biol. 1997 Oct 6. 139(1):145-56.
  PUBMED    9314535
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 1997 Oct 6. 139(1):145-56."
COMMENT     Sequence Label: M15 pCS-twhh-nBeta gal
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..29
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(119..141)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tgactgctcc aaagaagaag cgtaaggtac cggtgggtga agaccagaaa cagcacctcg
       61 aactgagccg cgatattgcc cagcgtttca acgcgctgta tggcgagatc gatcccgtcg
      121 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
      181 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
      241 agttgcgcag cctgaatggc gaatggcgct ttgcctggtt tccggcacca gaagcggtgc
      301 cggaaagctg gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact
      361 ggcagatgca cggttacgat gcgcccatct acaccaacgt gacctatccc attacggtca
      421 atccgccgtt tgttcccacg gagaatccga cgggttgtta ctcgctcaca tttaatgttg
      481 atgaaagctg gctacaggaa ggccagacgc gaattatttt tgatggcgtt aactcggcgt
      541 ttcatctgtg gtgcaacggg cgctgggtcg gttacggcca ggacagtcgt ttgccgtctg
      601 aatttgacct gagcgcattt ttacgcgccg gagaaaaccg cctcgcggtg atggtgctgc
      661 gctggagtga cggcagttat ctggaagatc aggatatgtg gcggatgagc ggcattttcc
      721 gtgacgtctc gttgctgcat aaaccgacta cacaaatcag cgatttccat gttgc
//
