LOCUS       sequence-22936-e         764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Du SJ, Devoto SH, Westerfield M, Moon RT
  TITLE     Positive and negative regulation of muscle cell identity by members 
            of the hedgehog and TGF-beta gene families.
  JOURNAL   J Cell Biol. 1997 Oct 6. 139(1):145-56.
  PUBMED    9314535
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 1997 Oct 6. 139(1):145-56."
COMMENT     Sequence Label: M16 pCS-twhh-bGFP
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..715
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(32..60)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     655..675
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 catgagtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
       61 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
      121 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
      181 tgtcactact ttcacttatg gtgttcaatg cttttcaaga tacccagatc atatgaaacg
      241 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
      301 caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt
      361 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa
      421 attggaatac aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg
      481 aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga
      541 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
      601 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct
      661 tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaataatc
      721 tagtcgagcc tctagaacta tagtgagtcg tattacgtag atcc
//
