LOCUS       sequence-22938-e         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Du SJ, Devoto SH, Westerfield M, Moon RT
  TITLE     Positive and negative regulation of muscle cell identity by members 
            of the hedgehog and TGF-beta gene families.
  JOURNAL   J Cell Biol. 1997 Oct 6. 139(1):145-56.
  PUBMED    9314535
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 1997 Oct 6. 139(1):145-56."
COMMENT     Sequence Label: M18 pCS-twhh-nBeta gal Vec
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             22..42
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(132..154)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gagctcagaa aaatgactgc tccaaagaag aagcgtaagg taccggtggg tgaagaccag
       61 aaacagcacc tcgaactgag ccgcgatatt gcccagcgtt tcaacgcgct gtatggcgag
      121 atcgatcccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
      181 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
      241 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gctttgcctg gtttccggca
      301 ccagaagcgg tgccggaaag ctggctggag tgcgatcttc ctgaggccga tactgtcgtc
      361 gtcccctcaa actggcagat gcacggttac gatgcgccca tctacaccaa cgtgacctat
      421 cccattacgg tcaatccgcc gtttgttccc acggagaatc cgacgggttg ttactcgctc
      481 acatttaatg ttgatgaaag ctggctacag gaaggccaga cgcgaattat ttttgatggc
      541 gttaactcgg cgtttcatct gtggtgcaac gggcgctggg tcggttacgg ccaggacagt
      601 cgtttgccgt ctgaatttga cctgagcgca tttttacgcg ccggagaaaa ccgcctcgcg
      661 gtgatggtgc tgcgctggag tgacggcagt tatctggaag atcaggatat gtggcggatg
      721 agcggcattt tccgtgacgt ctcgttgctg
//
