LOCUS       sequence-229399-        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Part of GoldenGate Yarrowia lipolytica toolkit for assembling an 
            expression plasmid for Y. lipolytic yeast. Sequence for homologous 
            integration into Y. lipolytica genome. Linearization of cassette by 
            NotI restriction enzyme after the assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Larroude M, Park YK, Soudier P, Kubiak M, Nicaud JM, Rossignol T
  TITLE     A modular Golden Gate toolkit for Yarrowia lipolytica synthetic 
            biology.
  JOURNAL   Microb Biotechnol. 2019 May 31. doi: 10.1111/1751-7915.13427.
  PUBMED    31148366
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb 
            Biotechnol. 2019 May 31. doi: 10.1111/1751-7915.13427."
COMMENT     Sequence Label: GGE254
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggtctctgag tgctatttat cactctttac aacttctacc tcaactatct actttaataa
       61 atgaatatcg tttattctct atgattactg tatatgcgtt cctctaagac aaatcgaaac
      121 cagcatgcga tcgaatggca tacaaaagtt tcttccgaag ttgatcaatg tcctgatagt
      181 caggcagctt gagaagattg acacaggtgg aggccgtagg gaaccgatca acctgtctac
      241 cagcgttacg aatggcaaat gacgggttca aagccttgaa tccttgcaat ggtgccttgg
      301 atactgatgt cacaaactta agaagcagcc gcttgtcctc ttcctcgaaa ctctcaaaca
      361 cagtccagaa gtcctttata gtttgatctg tatccagata gcctccgtaa ttggtgtgtg
      421 tcttcaaatc ccagacgtcc acattggcat gtcctccact gataagcatt tgaagttcat
      481 ctgcgttgaa cattgaaacc cacgaagggt caatgagctg gtatagaccg cccaagaatg
      541 catctgtctg tgttctgata ctggtgttaa gcttgaagtt ggccacttca taaatatatc
      601 gaagatagtt ggccttagtg acaggtactt cgcttccgtt tcgaatcaga tcacgtgttc
      661 gaacattgcc ttcggagtca gcgtaatcga ttgaaaatgt aagaccaaga tcggcatctc
      721 cctgagtggc gtaaatctgt ttcagcgatg caagactctc gtagaggttg gggtccagag
      781 aatacagatc gtcaatcgac gaccgcatac ttccttgtgt ccacttttga aggaagaacg
      841 atgcaaagcc gtgctcaata agaatgttct cgtaaaggcc ctttcccacc agatttccca
      901 ggaatgcaag ttgtttagtg tcatgggtag tcgggttggg gtaaagaagg tggttatcgg
      961 tagcggcaaa aagtcctcga gaagtgttga atgcatcatc tgtgacagaa gtgcgagaga
     1021 cc
//
