LOCUS       sequence-229401-        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Part of GoldenGate Yarrowia lipolytica toolkit for assembling an 
            expression plasmid for Y. lipolytic yeast. Sequence for homologous 
            integration into Y. lipolytica genome. Linearization of cassette by 
            NotI restriction enzyme after the assembly .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Larroude M, Park YK, Soudier P, Kubiak M, Nicaud JM, Rossignol T
  TITLE     A modular Golden Gate toolkit for Yarrowia lipolytica synthetic 
            biology.
  JOURNAL   Microb Biotechnol. 2019 May 31. doi: 10.1111/1751-7915.13427.
  PUBMED    31148366
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb 
            Biotechnol. 2019 May 31. doi: 10.1111/1751-7915.13427."
COMMENT     Sequence Label: GGE252
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggtctctgag tgcagaagtt gagtgtttga atgtactact tgatagtagg gagaaggaaa
       61 aacctggtaa ttaatatatt gtcatgataa cgatactcaa gaagaggacg agtgatgcag
      121 cagtggaacg agtagttcga tgtgaggcaa acgtagcctg ctagttacgt accggtattg
      181 gaaccaatac gaaagtgaaa acgcacacat gttcagatgt tcttgcatca tgaagtgtca
      241 atattatctc accatgtctt gaaatagtac agtaagcagg tttagatact gttccatgtt
      301 gcattaatac atcgtcgaga tacaattatg atatagagct acagtatggc tctgaaaaga
      361 catgtatcga aagtaggata gagtctaagc acaagtaacg agctctgcgt tcgattcatg
      421 tctcaaattg tatacgcata gccatctatg acttgcgtca ccgccgtcgg ttgtctactt
      481 tcttgtctca ttcattgatg atataaataa cctaatcaac atgctatgtt ctcgcaaccc
      541 gctcctcctt ccatctttgt gctctctcgt gtcgcagatg gtatcgtgag cgagcctgtc
      601 tgtagtcggc cacaaattgg gccacgtcgg tgctatcgga tcctagaaac gactctcgaa
      661 gaatctcact aagctcgtcc gattccacag ctgaatgggt catactcgac tgtagagctg
      721 gacttgaaaa cggttgcaga gcccggtaca tttctgcctc cagctggacc cattctcctt
      781 ccaatttctt gcttgcagtc accttttctc ctactcgttg acgcagttca ggaagagtct
      841 cctggtactg ttccttgagt ttttgtgctc gctgttcgag atctttgagc gcgcgtgtct
      901 gtcggctgta gtctccgccg ttctcggcca caattgagcc cttgagcacg tcctgaggca
      961 cactggaaat gtgctgggtg gccagctcat cgagatgcga cagtgtgtac gtgcgagaga
     1021 cc
//
