LOCUS       sequence-229409-       12403 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Co-expression of mCitrine under the control of the CaMV 35S promoter
            and Derlin1 fused to mOrange2 (an ER-targeting marker protein) under
            the control of the A. thaliana ubiquitin-10 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12403)
  AUTHORS   Behrendorff JBYH, Sandoval-Ibanez OA, Sharma A, Pribil M
  TITLE     Membrane-bound protein scaffolding in diverse hosts using thylakoid 
            protein CURT1A.
  JOURNAL   ACS Synth Biol. 2019 Mar 18. doi: 10.1021/acssynbio.8b00418.
  PUBMED    30884945
REFERENCE   2  (bases 1 to 12403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2019 Mar 18. doi: 10.1021/acssynbio.8b00418."
COMMENT     Sequence Label: pN_35S/mCitrine/P_UBQ10/Derlin1-mOrange2
FEATURES             Location/Qualifiers
     source          1..12403
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1..708)
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGF
                     QTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNYE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     KIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     promoter        2769..3113
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     3057..3077
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             join(3213..3215,3216..3218,3219..3932)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3258..3279)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3519..3538)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3866..3887
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(3935..3959)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4279..4303
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5634..6263
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6692..7765
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7831..8025
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8261..8283)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8369..8509
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8424..8443
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8524..8541)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8695..9283)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8775..8794)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9529..10320)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    10841..10865
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(10958..11210)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(11233..11805)
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     promoter        complement(11847..12026)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      complement(12136..12388)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 ttacttgtac agctcgtcca tgccgccggt ggagtggcgg ccctcggcgc gttcgtactg
       61 ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc aacttgatgt cgacgatgta
      121 ggcgccgggc agctgcacgg gcttcttggc cttgtaggtg gtcttgacct cggaggtgta
      181 gtggccgccg tccttcagct tcagcctcat cttgatcttg cccttcaggg caccgtcctc
      241 ggggtacatc cgctcggagg aggcctccca gcccatggtc ttcttctgca tcacggggcc
      301 gtcggagggg aagttggtgc cgcgcagctt caccttgtag atgaactcgc cgtcctgcag
      361 ggaggagtcc tgggtcacgg tcaccacgcc gccgtcctcg tagttcatca cgcgctccca
      421 cttgaagccc tcggggaagg acagcttgaa gtagtcgggg atgtcggcgg ggtgcttcac
      481 gtaggccttg gagccgtagg tgaaatgagg ggacaggatg tcccaggcga agggcagggg
      541 gccacccttg gtcaccttca gcttagcggt ctgaaagccc tcgtaggggc ggccctcgcc
      601 ctcgccctcg atctcgaact cgtggccgtt cacggagccc tccatgcgca ccttgaagcg
      661 catgaactcc ttgatgatgg ccatgttatt ctcctcgccc ttgctcacag atccaccacc
      721 tccgtcggtg agacgatatg atcgacctct gaatgctgtg tttgaactct cgggtggggc
      781 acgagcgctt gaataagctc ctcctcctcc tactcctcct ccagctccgg gtcctgctgc
      841 tcctacacca cctgcttgtc gaacacttgc aactggagct ccaattcgcc atcttgctac
      901 gattttatta acccattttg gggttttcag gtagtttttt ccggtagcaa gaggatgaag
      961 gactgttagg aagtagtata gatgtccagc tatgatgcca agaagatctg gcatgatcgg
     1021 agaaccgaag ataacatcaa gtgctagcat tgcccatggg aggtagaaag ccttaagagt
     1081 gacaagaccg tataggctga tgttggcatt tggaaattct cggctccaga gataaagaag
     1141 catgaacaca agtgaaacac caagaaacgg tgtccagaaa aatggtatga ctgataatac
     1201 caatagagtg aaggatccaa agatcatcat ccacaaaaag tctgctgtcc gcctttcaaa
     1261 tggccctttc tcgagttgaa ctccatatct tgctatcatc aaaagacgta ttccaaaatt
     1321 gatagagaaa ccaccgagga aaaacaagtt tgttatcagc ctccagatct ggaactgctt
     1381 aagtacaagt tcaggaatta aagcaatgtg aacgggagca actaagccaa gctgcgtggc
     1441 tactgtagtg aaaaagcaca acgtaccata agccttggtt ataggtggaa gtgagttata
     1501 gaattcgcca ggagaagaca tctgttaatc agaaaaactc agattaatcg acaaattcga
     1561 tcgcacaaac tagaaactaa caccagatct agatagaaat cacaaatcga agagtaatta
     1621 ttcgacaaaa ctcaaattat ttgaacaaat cggatgatat ctatgaaacc ctaatcgaga
     1681 attaagatga tatctaacga tcaaacccag aaaatcgtct tcgatctaag attaacagaa
     1741 tctaaaccaa agaacatata cgaaattggg atcgaacgaa aacaaaatcg aagattttga
     1801 gagaataagg aacacagaaa tttaccttga tcacggtaga gagaattgag agaaagtttt
     1861 taagattttg agaaattgaa atctgaattg tgaagaagaa gagctctttg ggtattgttt
     1921 tatagaagaa gaagaagaaa agacgaggac gactaggtca cgagaaagct aaggcggtga
     1981 agcaatagct aataataaaa tgacacgtgt attgagcgtt gtttacacgc aaagttgttt
     2041 ttggctaatt gccttatttt taggttgagg aaaagtattt gtgctttgag ttgataaaca
     2101 cgactcgtgt gtgccggctg caaccacttt gacgccgttt attactgact cgtcgacact
     2161 agagccaagc tgatctcctt tgccccggag atcaccatgg acgactttct ctatctctac
     2221 gatctaggaa gaaagttcga cggagaaggt gacgatacca tgttcaccac cgataatgag
     2281 aagattagcc tcttcaattt cagaaagaat gctgacccac agatggttag agaggcctac
     2341 gcggcaggtc tcatcaagac gatctacccg agtaataatc tccaggagat caaatacctt
     2401 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag
     2461 aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
     2521 cataaaccaa ggcaagtaat agagattgga gtctctaaga aagtagttcc tactgaatca
     2581 aaggccatgg agtcaaaaat tcagatcgag gatctaacag aactcgccgt gaagactggc
     2641 gaacagttca tacagagtct tttacgactc aatgacaaga agaaaatctt cgtcaacatg
     2701 gtggagcacg acactctcgt ctactccaag aatatcaaag atacagtctc agaagaccaa
     2761 agggctattg agacttttca acaaagggta atatcgggaa acctcctcgg attccattgc
     2821 ccagctatct gtcacttcat caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc
     2881 catcattgcg ataaaggaaa ggctatcgtt caagatgccc ctgccgacag tggtcccaaa
     2941 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     3001 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     3061 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag gactccggta
     3121 tttttacaac aattaccaca acaaaacaaa caacaaacaa cattacaatt tactattcta
     3181 gtcgacctgc aggcggccgc actagtgata tcatggtgag caagggcgag gagctgttca
     3241 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     3301 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     3361 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctga
     3421 tgtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     3481 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     3541 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     3601 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     3661 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     3721 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     3781 gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc aagctgagca
     3841 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     3901 tcactctcgg catggacgag ctgtacaagt aatcgttcag ctttcttgta caaagtggtg
     3961 atatcccgcg gccatgctag agtccgcaaa aatcaccagt ctctctctac aaatctatct
     4021 ctctctattt ttctccagaa taatgtgtga gtagttccca gataagggaa ttagggttct
     4081 tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt
     4141 aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagtg acctgcaggc
     4201 atgcgacgtc gggcccaagc ttagcttgag cttggatcag attgtcgttt cccgccttca
     4261 gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt
     4321 ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt
     4381 atgtgcatgc caaccacagg gttcccctcg ggatcaaagt actttaaagt actttaaagt
     4441 actttaaagt actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc
     4501 agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc
     4561 cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac
     4621 ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg
     4681 ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc
     4741 ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga
     4801 gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga
     4861 ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg
     4921 cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat
     4981 ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac
     5041 ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct
     5101 caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa
     5161 agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag
     5221 cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac
     5281 cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg
     5341 caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc
     5401 gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc
     5461 ctggccggtt tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa
     5521 accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg
     5581 gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg
     5641 ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag
     5701 cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca
     5761 gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc
     5821 gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg
     5881 gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga
     5941 ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta
     6001 agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga
     6061 tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca
     6121 ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa
     6181 ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg
     6241 aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa
     6301 cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct
     6361 ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa
     6421 gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat
     6481 cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta
     6541 aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt
     6601 gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg
     6661 gcactggaac ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt
     6721 acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc
     6781 ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga
     6841 tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc
     6901 gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg
     6961 cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc
     7021 tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc
     7081 cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga
     7141 atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca
     7201 cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct
     7261 ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc
     7321 caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat
     7381 cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg attggatgta
     7441 ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt
     7501 gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc
     7561 agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa
     7621 gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt
     7681 gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga
     7741 gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc
     7801 aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc acgtacattg ggaacccaaa
     7861 gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg
     7921 gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc
     7981 tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg
     8041 cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc
     8101 cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa atggctggcc tacggccagg
     8161 caatctacca gggcgcggac aagccgcgcc gtcgccactc gaccgccggc gcccacatca
     8221 aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     8281 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     8341 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg
     8401 gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     8461 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc
     8521 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     8581 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     8641 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     8701 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     8761 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     8821 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     8881 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     8941 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     9001 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     9061 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     9121 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     9181 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     9241 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     9301 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgca
     9361 tgatatatct cccaatttgt gtagggctta ttatgcacgc ttaaaaataa taaaagcaga
     9421 cttgacctga tagtttggct gtgagcaatt atgtgcttag tgcatctaac gcttgagtta
     9481 agccgcgccg cgaagcggcg tcggcttgaa cgaatttcta gctagacatt atttgccgac
     9541 taccttggtg atctcgcctt tcacgtagtg gacaaattct tccaactgat ctgcgcgcga
     9601 ggccaagcga tcttcttctt gtccaagata agcctgtcta gcttcaagta tgacgggctg
     9661 atactgggcc ggcaggcgct ccattgccca gtcggcagcg acatccttcg gcgcgatttt
     9721 gccggttact gcgctgtacc aaatgcggga caacgtaagc actacatttc gctcatcgcc
     9781 agcccagtcg ggcggcgagt tccatagcgt taaggtttca tttagcgcct caaatagatc
     9841 ctgttcagga accggatcaa agagttcctc cgccgctgga cctaccaagg caacgctatg
     9901 ttctcttgct tttgtcagca agatagccag atcaatgtcg atcgtggctg gctcgaagat
     9961 acctgcaaga atgtcattgc gctgccattc tccaaattgc agttcgcgct tagctggata
    10021 acgccacgga atgatgtcgt cgtgcacaac aatggtgact tctacagcgc ggagaatctc
    10081 gctctctcca ggggaagccg aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt
    10141 ttcatcaagc cttacggtca ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc
    10201 gccatccact gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc
    10261 gatgacgcca actacctctg atagttgagt cgatacttcg gcgatcaccg cttcccccat
    10321 gatgtttaac tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa
    10381 catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg
    10441 taccccaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct
    10501 gcgttcggtc aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagctt
    10561 acgaaccgaa caggcttatg tccactgggt tcgtgcccga attgatcaca ggcagcaacg
    10621 ctctgtcatc gttacaatca acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg
    10681 gcagcttagt tgccgttctt ccgaatagca tcggtaacat gagcaaagtc tgccgcctta
    10741 caacggctct cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt
    10801 ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg tggcaggata tattgtggtg
    10861 taaacaaatt gacgcttaga caacttaata acacattgcg gacgttttta atgtactgaa
    10921 ttaacgccga attgaattat cagcttgcat gccggtcgat ctagtaacat agatgacacc
    10981 gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc gtattaaatg
    11041 tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat gcattacatg
    11101 ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat cgcaagaccg
    11161 gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc tgcttgactc
    11221 taggggtcat cattaggggc agggcatgct catgtagagc gcctgctcgc cgtccgaggc
    11281 ggtgccgtcg tacagggcgg tgtccaggcc gcagagggtg aaccccatcc gccggtacgc
    11341 gtggatcgcc ggtgcgttga cgttggtgac ctccagccag aggtgcccgg caccccgctc
    11401 gcgggcgaac tccgtcgcga gccccatcaa cgcgcgcccg accccgtgcc cccggtgctc
    11461 cggggcgacc tcgatgtcct cgacggtcag ccggcggttc cagccggagt acgagacgac
    11521 cacgaagccc gccaggtcgc cgtcgtcccc gtacgcgacg aacgtccgag agtccgggtc
    11581 gccgtcctcc ccgtcgtccg attcgtcgtc cgattcgtcg tcggggaaca ccttggtcag
    11641 gggcgggtcc accggcacct cccgcagggt gaagccgtcc ccggtggcgg tgacgcggaa
    11701 gacggtgtcg gtggtgaagg acccatccag tgcctcgatg gcctcggcgt cccccgggac
    11761 actggtgcgg taccggtaag ccgtgtcgtc aagagtggta cccatggtag atcccctcga
    11821 tcgagttgag agtgaatatg agactctaat tggataccga ggggaattta tggaacgtca
    11881 gtggagcatt tttgacaaga aatatttgct agctgatagt gaccttaggc gacttttgaa
    11941 cgcgcaataa tggtttctga cgtatgtgct tagctcatta aactccagaa acccgcggct
    12001 cagtggctcc ttcaacgttg cggttctgtc agttccaaac gtaaaacggc ttgtcccgcg
    12061 tcatcggcgg gggtcataac gtgactccct taattctccg ctcatgatcg ataattcgag
    12121 ctctcccata tggtcgatct agtaacatag atgacaccgc gcgcgataat ttatcctagt
    12181 ttgcgcgcta tattttgttt tctatcgcgt attaaatgta taattgcggg actctaatca
    12241 taaaaaccca tctcataaat aacgtcatgc attacatgtt aattattaca tgcttaacgt
    12301 aattcaacag aaattatatg ataatcatcg caagaccggc aacaggattc aatcttaaga
    12361 aactttattg ccaaatgttt gaacgatctg ccaactttgt aca
//
