LOCUS       sequence-229413-        5054 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the His tagged Ornithine Decarboxylase from Saccharomyces 
            cerevisiae in Escherichia coli BL21 or similar strains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5054)
  AUTHORS   Markus Ralser
  TITLE     His tagged ScOrnithine Decarboxylase_pET-20b(+)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: ScSpe1_pET20b(+)
FEATURES             Location/Qualifiers
     source          1..5054
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     164..183
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        164..182
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             214..236
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     sig_peptide     244..309
                     /codon_start=1
                     /gene="pelB (fragment)"
                     /product="leader peptide for secretion"
                     /label=pelB signal sequence
                     /translation="MKYLLPTAAAGLLLLAAQPAMA"
     CDS             1718..1735
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1788..1806)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1802..1849
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1886..2341
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1973..1992)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2183..2204
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2367..2471
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2472..2540,2541..3332)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2690..2709)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3503..4091
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3992..4011
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4245..4262
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4277..4416
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4342..4361)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4502..4524
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(4518..4709)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt
       61 aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg
      121 tggccaggac ccaacgctgc ccgagatctc gatcccgcga aattaatacg actcactata
      181 gggagaccac aacggtttcc ctctagaaat aattttgttt aactttaaga aggagatata
      241 catatgaaat acctgctgcc gaccgctgct gctggtctgc tgctcctcgc tgcccagccg
      301 gcgatggcca tggatgtcta gtactcaagt aggaaatgct ctatctagtt ccactactac
      361 tttagtggac ttgtctaatt ctacggttac ccaaaagaag caatattata aagatggcga
      421 gacgctgcac aatcttttgc ttgaactaaa gaataaccaa gatttggaac ttttaccgca
      481 tgaacaagcg catcctaaaa tatttcaagc gctcaaggct cgtattggta gaattaataa
      541 tgaaacgtgc gaccccggtg aggagaactc gtttttcata tgcgatttgg gagaagtcaa
      601 gagattattc aacaactggg tgaaggagct tcctagaatt aagccatttt atgccgtcaa
      661 atgtaatcct gataccaagg ttttgtcatt attagcagag ttgggcgtta atttcgattg
      721 cgcttccaaa gtggaaattg acagagtatt atcgatgaac atctcgccgg atagaattgt
      781 ttacgctaat ccttgtaaag tagcatcttt cattagatat gcagcttcaa aaaatgtaat
      841 gaagtctact tttgacaatg tagaagaatt gcataaaatc aaaaagtttc atcctgagtc
      901 tcagttgtta ttaagaatcg ctaccgatga ctctaccgct caatgtcgac tttccaccaa
      961 atatggctgt gaaatggaaa acgtagacgt tttattaaag gctataaagg aactaggttt
     1021 aaacctggct ggtgtttctt tccacgtcgg ttcaggcgct tctgatttta caagcttata
     1081 caaagccgtt agagatgcaa gaacggtatt tgacaaagct gctaacgaat acgggttgcc
     1141 ccctttgaag attttggatg taggtggtgg atttcaattt gaatccttca aagaatcaac
     1201 tgctgttttg cgtctagcgc tagaggaatt tttccctgta ggttgtggtg ttgatataat
     1261 tgcagagcct ggcagatact ttgtagctac agcgttcact ttggcatctc atgtgattgc
     1321 gaagagaaaa ctgtctgaga atgaagcaat gatttacact aacgatggtg tatacgggaa
     1381 catgaattgt attttattcg atcatcaaga gccccatcca agaacccttt atcataattt
     1441 ggaatttcat tacgacgatt ttgaatccac tactgcggtc ctcgactcta tcaacaaaac
     1501 aagatctgag tatccatata aagtttccat ctggggaccc acatgtgatg gtttggattg
     1561 tattgccaaa gagtattaca tgaagcatga tgttatagtc ggtgattggt tttattttcc
     1621 tgccctgggt gcctacacat catcggcggc tactcaattc aacggctttg agcagactgc
     1681 ggatatagta tacatagact ctgaactcga tctcgagcac caccaccacc accactgaga
     1741 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
     1801 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
     1861 aactatatcc ggattggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     1921 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     1981 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     2041 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     2101 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     2161 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     2221 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     2281 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     2341 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     2401 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     2461 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     2521 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     2581 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     2641 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     2701 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     2761 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     2821 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     2881 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     2941 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     3001 acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     3061 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     3121 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     3181 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     3241 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     3301 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     3361 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     3421 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     3481 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     3541 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     3601 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     3661 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     3721 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     3781 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     3841 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     3901 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     3961 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     4021 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     4081 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     4141 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     4201 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     4261 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     4321 accgcaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac
     4381 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct
     4441 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     4501 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg
     4561 cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg
     4621 tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg
     4681 ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc
     4741 atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat
     4801 gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg
     4861 gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt
     4921 ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct
     4981 gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct
     5041 caggtcgcag acgt
//
