LOCUS       sequence-229422-       12963 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human BRAF V600E mutant and zebrafish mitfa specifically 
            in zebrafish melanocytes .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12963)
  AUTHORS   Ablain J, Xu M, Rothschild H, Jordan RC, Mito JK, Daniels BH, Bell 
            CF, Joseph NM, Wu H, Bastian BC, Zon LI, Yeh I
  TITLE     Human tumor genomics and zebrafish modeling identify SPRED1 loss as 
            a driver of mucosal melanoma.
  JOURNAL   Science. 2018 Nov 1. pii: science.aau6509. doi: 
            10.1126/science.aau6509.
  PUBMED    30385465
REFERENCE   2  (bases 1 to 12963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2018 Nov 1. pii: science.aau6509. doi: 10.1126/science.aau6509."
COMMENT     Sequence Label: MiniCoopR mitfa:BRAF
FEATURES             Location/Qualifiers
     source          1..12963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(514..525,526..688)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     1061..1077
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1097..1117
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2991..3015
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5285..5309)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    5311..5445
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(5508..5528)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(5536..5552)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    5568..5702
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(10493..10680,10681..10692)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(10726..10742)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10726..10742)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(10779..10799)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10779..10797)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10818..10834)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10831..10853)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10842..10858
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10866..10872,10873..10890,10891..10896))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10911..10932
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11049..11066)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11220..11808)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11300..11319)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11979..12770,12771..12839))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12602..12621
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12840..12944)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 atgctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggccagatg ggccctcgag caggaaacag ctatgaccat
     1081 gattacgcca agctatcaac tttgtataga aaagttgtga gtgctaacaa ctgggctgct
     1141 gtgccgctct ttcgaggaaa tgaaaggaac tctggcggcc tatgatgagt taggaattgt
     1201 ctgattggtg aatcatacat aggctaatgt gggaccagct gtagtcagtc ataagcacgt
     1261 gattctctcg aaattagttt ataaataaac ttcactaaac tccaaccgtt cattctgttt
     1321 tagatgttgc ctttaaactt gccttaagat gttaataaag ttgctttttg gttgttgaca
     1381 aagctaggca taggacgata accgttttca aggtataccc cggtttgaaa aagtcaaggg
     1441 tttaaaacca ccaatatttt ctgctatacc gttcctaagg tatatttaag attttttatt
     1501 tattttttat ttttttgttt tttaggacaa cagcattttc agcagaaaag agatacaaaa
     1561 atgcttttta aattgtaaag aaatctgttt ttgaaactaa tgaagacagc agaagtcgat
     1621 cattcattga aattattaag cttgacatgt tttctgttcc aaaatatttt ttcaatgttt
     1681 ctcaaaataa aatatattat gtttaaagtt tttgtttttt actaagacat taaaaaaaat
     1741 aatatatttt agagtggcaa tcacaatacc atcaaactgt gatattttta tcaaaggtta
     1801 tcataccatc agaatcttat accccatgcc tagacacagc tgttctctct atctctctat
     1861 gttaatgcag tttccactcc attgacgaga tggttctctg taccacatac tcatccccat
     1921 tttacaatag ctacatttgc tcaagatgta gtcaactcaa acaatgcaga gacaacaaac
     1981 tgttgactta tcttttctat cgtaattcag cccttttggg aaatgagcat caacacacaa
     2041 tttagagtgg aagttgggtt aaggcagact cattttttac acattacaac cagtgattat
     2101 gtcaattatt atttaaaaaa aattcagtac agtatatgca catgctactt tgtttatgat
     2161 tgttagcttt tttaactaca gttcatttct atgctttctg tgcaaatgca caacagttac
     2221 tgatttactc tgatgataat tgtgccagta catccaacaa caacaaaaaa aaaaaaaaga
     2281 cttacaatca agccccaaag tgttcatacg tctggcaaat tctgacttaa agttactttt
     2341 attcaaccag ctagtttttt tttgactgta aatgacacgt aagataataa gatgatgtac
     2401 atgaggcatc attgtagaaa aaaaaatgaa tatgaatctg aatatgaact gtacgtctgt
     2461 gattttgttt gtgcgcactt acatacgtgt gcacaatatt tcaactctta agaaaaaaat
     2521 gcaattacca aaggcccatc agacaacaaa gttctctttc ctatcttttg ctcttcaaca
     2581 tttcagagat aactccatct taaactgtct tgtttttaga ccaacagtgc tagtgattgt
     2641 atgccggatg ctgagaacaa acaatgtttt atgcaggatg agtggaacta aagatgaaac
     2701 gttagttagc cgtaagccag tttcagttta gacactagta acccatcgtc tcaaagaggc
     2761 ttttgtcgaa tcggaccggt gacgtcaaat gaactttata aaggcataaa ttcatcgttt
     2821 gggtaaaaaa aacaatatga gaagagcaat cccctcccac ccccttaaat acattttgtg
     2881 tcttttcttt ggcattgaca tcttcagctg gccaagacga ctggtcagtt cttgcacatc
     2941 acctaaattc acaaagggaa tacttttttg aagctaacac atagttgaac gcaagtttgt
     3001 acaaaaaagc aggctccgcg gccgccccct tcaccagcta tccagcacag tggcggccgc
     3061 gaattcgccc ttatggcggc gctgagcggt ggcggtggtg gcggcgcgga gccgggccag
     3121 gctctgttca acggggacat ggagcccgag gccggcgccg gcgccggcgc cgcggcctct
     3181 tcggctgcgg accctgccat tccggaggag gcctatgaag aatacaccag caagctagat
     3241 gcactccaac aaagagaaca acagttattg gaatctctgg ggaacggaac tgatttttct
     3301 gtttctagct ctgcatcaat ggataccgtt acatcttctt cctcttctag cctttcagtg
     3361 ctaccttcat ctctttcagt ttttcaaaat cccacagatg tggcacggag caaccccaag
     3421 tcaccacaaa aacctatcgt tagagtcttc ctgcccaaca aacagaggac agtggtacct
     3481 gcaaggtgtg gagttacagt ccgagacagt ctaaagaaag cactgatgat gagaggtcta
     3541 atcccagagt gctgtgctgt ttacagaatt caggatggag agaagaaacc aattggttgg
     3601 gacactgata tttcctggct tactggagaa gaattgcatg tggaagtgtt ggagaatgtt
     3661 ccacttacaa cacacaactt tgtacgaaaa acgtttttca ccttagcatt ttgtgacttt
     3721 tgtcgaaagc tgcttttcca gggtttccgc tgtcaaacat gtggttataa atttcaccag
     3781 cgttgtagta cagaagttcc actgatgtgt gttaattatg accaacttga tttgctgttt
     3841 gtctccaagt tctttgaaca ccacccaata ccacaggaag aggcgtcctt agcagagact
     3901 gccctaacat ctggatcatc cccttccgca cccgcctcgg actctattgg gccccaaatt
     3961 ctcaccagtc cgtctccttc aaaatccatt ccaattccac agcccttccg accagcagat
     4021 gaagatcatc gaaatcaatt tgggcaacga gaccgatcct catcagctcc caatgtgcat
     4081 ataaacacaa tagaacctgt caatattgat gacttgatta gagaccaagg atttcgtggt
     4141 gatggaggat caaccacagg tttgtctgct accccccctg cctcattacc tggctcacta
     4201 actaacgtga aagccttaca gaaatctcca ggacctcagc gagaaaggaa gtcatcttca
     4261 tcctcagaag acaggaatcg aatgaaaaca cttggtagac gggactcgag tgatgattgg
     4321 gagattcctg atgggcagat tacagtggga caaagaattg gatctggatc atttggaaca
     4381 gtctacaagg gaaagtggca tggtgatgtg gcagtgaaaa tgttgaatgt gacagcacct
     4441 acacctcagc agttacaagc cttcaaaaat gaagtaggag tactcaggaa aacacgacat
     4501 gtgaatatcc tactcttcat gggctattcc acaaagccac aactggctat tgttacccag
     4561 tggtgtgagg gctccagctt gtatcaccat ctccatatca ttgagaccaa atttgagatg
     4621 atcaaactta tagatattgc acgacagact gcacagggca tggattactt acacgccaag
     4681 tcaatcatcc acagagacct caagagtaat aatatatttc ttcatgaaga cctcacagta
     4741 aaaataggtg attttggtct agctacagag aaatctcgat ggagtgggtc ccatcagttt
     4801 gaacagttgt ctggatccat tttgtggatg gcaccagaag tcatcagaat gcaagataaa
     4861 aatccataca gctttcagtc agatgtatat gcatttggaa ttgttctgta tgaattgatg
     4921 actggacagt taccttattc aaacatcaac aacagggacc agataatttt tatggtggga
     4981 cgaggatacc tgtctccaga tctcagtaag gtacggagta actgtccaaa agccatgaag
     5041 agattaatgg cagagtgcct caaaaagaaa agagatgaga gaccactctt tccccaaatt
     5101 ctcgcctcta ttgagctgct ggcccgctca ttgccaaaaa ttcaccgcag tgcatcagaa
     5161 ccctccttga atcgggctgg tttccaaaca gaggatttta gtctatatgc ttgtgcttct
     5221 ccaaaaacac ccatccaggc agggggatat ggtgcgtttc ctgtccacaa gggtgggcgc
     5281 gccgacccag ctttcttgta caaagtgggg gatccagaca tgataagata cattgatgag
     5341 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     5401 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     5461 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttccaa ctttattata
     5521 catagttgat aattcactgg ccgtcgtttt acggtaccat cgatgatgat ccagacatga
     5581 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     5641 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     5701 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
     5761 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcct ctagatcaga
     5821 tctcagattg tatccaaaat atcatttttt gtgttttaaa gatgaacaaa ggtctcaggg
     5881 aattggaacg acatgaggat gattaattaa taaaaaaaaa ttcatttttg ggtgaactat
     5941 ccctaaaatc aaagcaattt gggcattttt tttctaatta ttatggagaa cttgcatcac
     6001 agctcttcca ctagaaaata aaacaaattt cagaaatact gcagaaatac ttttggactt
     6061 aatgttaaat aacaatgtaa aaagtttttc cagggcatcg tggtggtgca gtgggtagca
     6121 ttgtcgcctt acagcaagaa ggtcgctggt tcgagacttg gttgggtcat ttctgtgtgg
     6181 agtttgcatg ttctccccat gttcgtgtgg gtttcctccg ggtgctccag tttcacctac
     6241 agcccaaaga catgcgctat aggtgaattg ggtaagctaa attgtctaaa gtgaatggta
     6301 ctgtggtggg tgtttcccag tgatgggttg cagctgcaag ggcatccgct ggatttgttt
     6361 gttggcataa gttggcagtt cattcaactg tggcaacccc tgattaataa aggggctaag
     6421 ccaaaaagaa aatgaatgac tgaatgaaag tgaatccagt tgtacatgcc tagacatgtc
     6481 ctccaccctt gcaagatgta caccccaaga aatggagctg catttgagga caacaataag
     6541 ccaagcctgc aaataaaaga cacctcaaca gtttttattc aagctactct ttatatcatc
     6601 cgcgagaatc cttcaaaaat caactaattg tttacacgat ttcattcttg actagactct
     6661 gacatgataa atgaaaaaat atatacacaa gcacactaaa tattctgaaa tacaagatta
     6721 atactttacg aaattgcgct cttcatgatc tggatgtaac actggtactt acaagacaca
     6781 cacacacaca aatctaattg tctaactttg gttcatgaaa tttagttggc attgctaaca
     6841 gccattgtca tgttcgtcca tactgctgct gccgctactg ttggaaggta ctggggatcc
     6901 ggaggacaac agcgggtcgc tgctgcccac cggagacagg gtgttgtcca taagcatgtc
     6961 ctccagcttg gtggaggcct tgtttgggct gtcgtagacc cccggctcgc cgtcctccgt
     7021 gggactgtca ttgtagctga tggtgccgtt gttgaggtcc agagtggtag gacgggacat
     7081 gtcaggactg ggaaggtgtg ggtacaaatt ggatgtgcag tcccccatcc caggctcttg
     7141 tttgattgct cgagccacta actcagcgga ataaaggctg gaagaagcta caacggtgag
     7201 tccatgagca cgggcttgca tttcaagttc ctgaatacgg agcaggagat gtctgtttgc
     7261 gtgttctagt ctcttctgtc tgttttccag ctcttttgct ttctgctgct ctttctgcaa
     7321 tttcctaatg taatccactg atgctttcag gatggtgcct ttattccacc tcatgtctgg
     7381 atcatttgac ttgggaatta aagtccccag ctccttaatt ctgtcattga tattaaaccg
     7441 ccttcttcgt tcaatgaggt tgtggttgtc cttcttttgt ctctcttttg ccagggctct
     7501 gacttctgct tctactggaa agccatcagg tctttgataa gagtcaaact tgccacatgg
     7561 tccagctttg tccattatgt gcatcatgcc cggagatgga gtaacggata attccctttt
     7621 gacggccggc aggcttgagg ggcaggagtt actgatggaa actccagctg gaggaagagc
     7681 atgattgctg tacatgtcca gcaggtttgc tgaaactgga atcgtgtttg tcatttgaag
     7741 tcctgcatcc atgaacccaa gaatgtcatc actgtagctt gattccaaac ttataatgtc
     7801 ttcaattaca tcgtccatct ctttctcaca gttgagggtg agaagggcca tagggctgtt
     7861 gggagcgctg gctccgggtc ccggggtcat gccgtgctcg gcgggctggc tggggccggg
     7921 ccctgtgctg gcctgtgggc tcagcttggc tcccagtgca ctggacaggt agtgcttcac
     7981 ctgctgcctc tggctctgct ggatgtggta cttcgagggg gtttccaggt gggtctgaac
     8041 ctggtagtga ctgtactcga gcatctccaa catgttcaac tatgtgttag cttcaaaaaa
     8101 gtattccctt tgtgaattta ggtgatgtgc aagaactgac cagtcgtctt ggccagctga
     8161 agatgtcaat gccaaagaaa agacacaaaa tgtatttaag ggggtgggag gggattgctc
     8221 ttctcatatt gtttttttta cccaaacgat gaatttatgc ctttataaag ttcatttgac
     8281 gtcaccggtc cgattcgaca aaagcctctt tgagacgatg ggttactagt gtctaaactg
     8341 aaactggctt acggctaact aacgtttcat ctttagttcc actcatcctg cataaaacat
     8401 tgtttgttct cagcatccgg catacaatca ctagcactgt tggtctaaaa acaagacagt
     8461 ttaagatgga gttatctctg aaatgttgaa gagcaaaaga taggaaagag aactttgttg
     8521 tctgatgggc ctttggtaat tgcatttttt tcttaagagt tgaaatattg tgcacacgta
     8581 tgtaagtgcg cacaaacaaa atcacagacg tacagttcat attcagattc atattcattt
     8641 ttttttctac aatgatgcct catgtacatc atcttattat cttacgtgtc atttacagtc
     8701 aaaaaaaaac tagctggttg aataaaagta actttaagtc agaatttgcc agacgtatga
     8761 acactttggg gcttgattgt aagtcttttt tttttttttg ttgttgttgg atgtactggc
     8821 acaattatca tcagagtaaa tcagtaactg ttgtgcattt gcacagaaag catagaaatg
     8881 aactgtagtt aaaaaagcta acaatcataa acaaagtagc atgtgcatat actgtactga
     8941 atttttttta aataataatt gacataatca ctggttgtaa tgtgtaaaaa atgagtctgc
     9001 cttaacccaa cttccactct aaattgtgtg ttgatgctca tttcccaaaa gggctgaatt
     9061 acgatagaaa agataagtca acagtttgtt gtctctgcat tgtttgagtt gactacatct
     9121 tgagcaaatg tagctattgt aaaatgggga tgagtatgtg gtacagagaa ccatctcgtc
     9181 aatggagtgg aaactgcatt aacatagaga gatagagaga acagctgtgt ctaggcatgg
     9241 ggtataagat tctgatggta tgataacctt tgataaaaat atcacagttt gatggtattg
     9301 tgattgccac tctaaaatat attatttttt ttaatgtctt agtaaaaaac aaaaacttta
     9361 aacataatat attttatttt gagaaacatt gaaaaaatat tttggaacag aaaacatgtc
     9421 aagcttaata atttcaatga atgatcgact tctgctgtct tcattagttt caaaaacaga
     9481 tttctttaca atttaaaaag catttttgta tctcttttct gctgaaaatg ctgttgtcct
     9541 aaaaaacaaa aaaataaaaa ataaataaaa aatcttaaat ataccttagg aacggtatag
     9601 cagaaaatat tggtggtttt aaacccttga ctttttcaaa ccggggtata ccttgaaaac
     9661 ggttatcgtc ctatgcctag ctttgtcaac aaccaaaaag caactttatt aacatcttaa
     9721 ggcaagttta aaggcaacat ctaaaacaga atgaacggtt ggagtttagt gaagtttatt
     9781 tataaactaa tttcgagaga atcacgtgct tatgactgac tacagctggt cccacattag
     9841 cctatgtatg attcaccaat cagacaattc ctaactcatc ataggccgcc agagttcctt
     9901 tcatttcctc gaaagagcgg cacagcagcc cagttgttag cactgttgcc tcaaagcaag
     9961 aacgtcactg gtccaagtct ctaacaggcc agtccatggt tctgtgtgga gtttacatgt
    10021 tgtcctcgtg ctcatgtgga tatcccctgg gttctccggt tttctcctac agtccaaaaa
    10081 cttgcgacat aggtgaattg aacaaaacca aattagcata gacaagctcc taatcagcag
    10141 aatatctctc cgtagcaatc tcttattcga gatctgcgaa gatacggcca cgggtgctct
    10201 tgatcctgtg gctgattttg gactgtgctg ctcgcagctg ctgatgaatc acatacttcc
    10261 tccattttct tccactgatt gactgttata atttccctaa tttccaggtc aaggtgctgt
    10321 gcattgtggt aatagatgtg acatgacgtc acttccaaag gaccaatgaa catgtctgac
    10381 caatttcata taatgtgaaa acgattttca taggcagaat aaataacatt taaattaaac
    10441 tgggcatcag cgcaattcaa ttggtttggt aatagcaagg gaaaatagaa tgaagtgatc
    10501 tccaaaaaat aagtactttt tgactgtaaa taaaattgta aggagtaaaa agtacttttt
    10561 tttctaaaaa aatgtaatta agtaaaagta aaagtattga tttttaattg tactcaagta
    10621 aagtaaaaat ccccaaaaat aatacttaag tacagtaatc aagtaaaatt actcaagtac
    10681 tttacacctc tggttcttga ccccctacct tcagcaagcc cagcagatcc actagttcta
    10741 gagcggccgc caccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattgcg
    10801 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
    10861 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
    10921 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
    10981 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
    11041 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
    11101 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
    11161 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
    11221 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
    11281 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
    11341 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
    11401 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
    11461 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
    11521 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
    11581 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
    11641 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
    11701 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
    11761 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
    11821 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg cattttggtc
    11881 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
    11941 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
    12001 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
    12061 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
    12121 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
    12181 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
    12241 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
    12301 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    12361 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    12421 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    12481 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    12541 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
    12601 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    12661 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    12721 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    12781 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    12841 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    12901 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    12961 gtg
//
