LOCUS       sequence-229430-       11477 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses EGFP and zebrafish mitfa specifically in zebrafish 
            melanocytes .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11477)
  AUTHORS   Ablain J, Xu M, Rothschild H, Jordan RC, Mito JK, Daniels BH, Bell 
            CF, Joseph NM, Wu H, Bastian BC, Zon LI, Yeh I
  TITLE     Human tumor genomics and zebrafish modeling identify SPRED1 loss as 
            a driver of mucosal melanoma.
  JOURNAL   Science. 2018 Nov 1. pii: science.aau6509. doi: 
            10.1126/science.aau6509.
  PUBMED    30385465
REFERENCE   2  (bases 1 to 11477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2018 Nov 1. pii: science.aau6509. doi: 10.1126/science.aau6509."
COMMENT     Sequence Label: MiniCoopR mitfa:EGFP
FEATURES             Location/Qualifiers
     source          1..11477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(514..525,526..688)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     1061..1077
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1097..1117
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2991..3015
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(3021..3023,3024..3026,3027..3737)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3021..3023,3024..3026,3027..3737)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3066..3087)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3327..3346)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3674..3695
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(3799..3823)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    3825..3959
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(4022..4042)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(4050..4066)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    4082..4216
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(9007..9194,9195..9206)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(9240..9256)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9240..9256)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(9293..9313)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9293..9311)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9332..9348)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9345..9367)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9356..9372
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9380..9386,9387..9404,9405..9410))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9425..9446
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9563..9580)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9734..10322)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9814..9833)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10493..11284,11285..11353))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11116..11135
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11354..11458)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 atgctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggccagatg ggccctcgag caggaaacag ctatgaccat
     1081 gattacgcca agctatcaac tttgtataga aaagttgtga gtgctaacaa ctgggctgct
     1141 gtgccgctct ttcgaggaaa tgaaaggaac tctggcggcc tatgatgagt taggaattgt
     1201 ctgattggtg aatcatacat aggctaatgt gggaccagct gtagtcagtc ataagcacgt
     1261 gattctctcg aaattagttt ataaataaac ttcactaaac tccaaccgtt cattctgttt
     1321 tagatgttgc ctttaaactt gccttaagat gttaataaag ttgctttttg gttgttgaca
     1381 aagctaggca taggacgata accgttttca aggtataccc cggtttgaaa aagtcaaggg
     1441 tttaaaacca ccaatatttt ctgctatacc gttcctaagg tatatttaag attttttatt
     1501 tattttttat ttttttgttt tttaggacaa cagcattttc agcagaaaag agatacaaaa
     1561 atgcttttta aattgtaaag aaatctgttt ttgaaactaa tgaagacagc agaagtcgat
     1621 cattcattga aattattaag cttgacatgt tttctgttcc aaaatatttt ttcaatgttt
     1681 ctcaaaataa aatatattat gtttaaagtt tttgtttttt actaagacat taaaaaaaat
     1741 aatatatttt agagtggcaa tcacaatacc atcaaactgt gatattttta tcaaaggtta
     1801 tcataccatc agaatcttat accccatgcc tagacacagc tgttctctct atctctctat
     1861 gttaatgcag tttccactcc attgacgaga tggttctctg taccacatac tcatccccat
     1921 tttacaatag ctacatttgc tcaagatgta gtcaactcaa acaatgcaga gacaacaaac
     1981 tgttgactta tcttttctat cgtaattcag cccttttggg aaatgagcat caacacacaa
     2041 tttagagtgg aagttgggtt aaggcagact cattttttac acattacaac cagtgattat
     2101 gtcaattatt atttaaaaaa aattcagtac agtatatgca catgctactt tgtttatgat
     2161 tgttagcttt tttaactaca gttcatttct atgctttctg tgcaaatgca caacagttac
     2221 tgatttactc tgatgataat tgtgccagta catccaacaa caacaaaaaa aaaaaaaaga
     2281 cttacaatca agccccaaag tgttcatacg tctggcaaat tctgacttaa agttactttt
     2341 attcaaccag ctagtttttt tttgactgta aatgacacgt aagataataa gatgatgtac
     2401 atgaggcatc attgtagaaa aaaaaatgaa tatgaatctg aatatgaact gtacgtctgt
     2461 gattttgttt gtgcgcactt acatacgtgt gcacaatatt tcaactctta agaaaaaaat
     2521 gcaattacca aaggcccatc agacaacaaa gttctctttc ctatcttttg ctcttcaaca
     2581 tttcagagat aactccatct taaactgtct tgtttttaga ccaacagtgc tagtgattgt
     2641 atgccggatg ctgagaacaa acaatgtttt atgcaggatg agtggaacta aagatgaaac
     2701 gttagttagc cgtaagccag tttcagttta gacactagta acccatcgtc tcaaagaggc
     2761 ttttgtcgaa tcggaccggt gacgtcaaat gaactttata aaggcataaa ttcatcgttt
     2821 gggtaaaaaa aacaatatga gaagagcaat cccctcccac ccccttaaat acattttgtg
     2881 tcttttcttt ggcattgaca tcttcagctg gccaagacga ctggtcagtt cttgcacatc
     2941 acctaaattc acaaagggaa tacttttttg aagctaacac atagttgaac gcaagtttgt
     3001 acaaaaaagc aggctggacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     3061 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     3121 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     3181 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     3241 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     3301 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     3361 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     3421 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     3481 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     3541 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     3601 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     3661 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     3721 tggacgagct gtacaagggc ggtggaagat ctgggaattc aaggcctctc gagcctctag
     3781 attctgcagc cctatagcac ccagctttct tgtacaaagt gggggatcca gacatgataa
     3841 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     3901 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     3961 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     4021 ccaactttat tatacatagt tgataattca ctggccgtcg ttttacggta ccatcgatga
     4081 tgatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     4141 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     4201 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     4261 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga
     4321 tcctctagat cagatctcag attgtatcca aaatatcatt ttttgtgttt taaagatgaa
     4381 caaaggtctc agggaattgg aacgacatga ggatgattaa ttaataaaaa aaaattcatt
     4441 tttgggtgaa ctatccctaa aatcaaagca atttgggcat tttttttcta attattatgg
     4501 agaacttgca tcacagctct tccactagaa aataaaacaa atttcagaaa tactgcagaa
     4561 atacttttgg acttaatgtt aaataacaat gtaaaaagtt tttccagggc atcgtggtgg
     4621 tgcagtgggt agcattgtcg ccttacagca agaaggtcgc tggttcgaga cttggttggg
     4681 tcatttctgt gtggagtttg catgttctcc ccatgttcgt gtgggtttcc tccgggtgct
     4741 ccagtttcac ctacagccca aagacatgcg ctataggtga attgggtaag ctaaattgtc
     4801 taaagtgaat ggtactgtgg tgggtgtttc ccagtgatgg gttgcagctg caagggcatc
     4861 cgctggattt gtttgttggc ataagttggc agttcattca actgtggcaa cccctgatta
     4921 ataaaggggc taagccaaaa agaaaatgaa tgactgaatg aaagtgaatc cagttgtaca
     4981 tgcctagaca tgtcctccac ccttgcaaga tgtacacccc aagaaatgga gctgcatttg
     5041 aggacaacaa taagccaagc ctgcaaataa aagacacctc aacagttttt attcaagcta
     5101 ctctttatat catccgcgag aatccttcaa aaatcaacta attgtttaca cgatttcatt
     5161 cttgactaga ctctgacatg ataaatgaaa aaatatatac acaagcacac taaatattct
     5221 gaaatacaag attaatactt tacgaaattg cgctcttcat gatctggatg taacactggt
     5281 acttacaaga cacacacaca cacaaatcta attgtctaac tttggttcat gaaatttagt
     5341 tggcattgct aacagccatt gtcatgttcg tccatactgc tgctgccgct actgttggaa
     5401 ggtactgggg atccggagga caacagcggg tcgctgctgc ccaccggaga cagggtgttg
     5461 tccataagca tgtcctccag cttggtggag gccttgtttg ggctgtcgta gacccccggc
     5521 tcgccgtcct ccgtgggact gtcattgtag ctgatggtgc cgttgttgag gtccagagtg
     5581 gtaggacggg acatgtcagg actgggaagg tgtgggtaca aattggatgt gcagtccccc
     5641 atcccaggct cttgtttgat tgctcgagcc actaactcag cggaataaag gctggaagaa
     5701 gctacaacgg tgagtccatg agcacgggct tgcatttcaa gttcctgaat acggagcagg
     5761 agatgtctgt ttgcgtgttc tagtctcttc tgtctgtttt ccagctcttt tgctttctgc
     5821 tgctctttct gcaatttcct aatgtaatcc actgatgctt tcaggatggt gcctttattc
     5881 cacctcatgt ctggatcatt tgacttggga attaaagtcc ccagctcctt aattctgtca
     5941 ttgatattaa accgccttct tcgttcaatg aggttgtggt tgtccttctt ttgtctctct
     6001 tttgccaggg ctctgacttc tgcttctact ggaaagccat caggtctttg ataagagtca
     6061 aacttgccac atggtccagc tttgtccatt atgtgcatca tgcccggaga tggagtaacg
     6121 gataattccc ttttgacggc cggcaggctt gaggggcagg agttactgat ggaaactcca
     6181 gctggaggaa gagcatgatt gctgtacatg tccagcaggt ttgctgaaac tggaatcgtg
     6241 tttgtcattt gaagtcctgc atccatgaac ccaagaatgt catcactgta gcttgattcc
     6301 aaacttataa tgtcttcaat tacatcgtcc atctctttct cacagttgag ggtgagaagg
     6361 gccatagggc tgttgggagc gctggctccg ggtcccgggg tcatgccgtg ctcggcgggc
     6421 tggctggggc cgggccctgt gctggcctgt gggctcagct tggctcccag tgcactggac
     6481 aggtagtgct tcacctgctg cctctggctc tgctggatgt ggtacttcga gggggtttcc
     6541 aggtgggtct gaacctggta gtgactgtac tcgagcatct ccaacatgtt caactatgtg
     6601 ttagcttcaa aaaagtattc cctttgtgaa tttaggtgat gtgcaagaac tgaccagtcg
     6661 tcttggccag ctgaagatgt caatgccaaa gaaaagacac aaaatgtatt taagggggtg
     6721 ggaggggatt gctcttctca tattgttttt tttacccaaa cgatgaattt atgcctttat
     6781 aaagttcatt tgacgtcacc ggtccgattc gacaaaagcc tctttgagac gatgggttac
     6841 tagtgtctaa actgaaactg gcttacggct aactaacgtt tcatctttag ttccactcat
     6901 cctgcataaa acattgtttg ttctcagcat ccggcataca atcactagca ctgttggtct
     6961 aaaaacaaga cagtttaaga tggagttatc tctgaaatgt tgaagagcaa aagataggaa
     7021 agagaacttt gttgtctgat gggcctttgg taattgcatt tttttcttaa gagttgaaat
     7081 attgtgcaca cgtatgtaag tgcgcacaaa caaaatcaca gacgtacagt tcatattcag
     7141 attcatattc attttttttt ctacaatgat gcctcatgta catcatctta ttatcttacg
     7201 tgtcatttac agtcaaaaaa aaactagctg gttgaataaa agtaacttta agtcagaatt
     7261 tgccagacgt atgaacactt tggggcttga ttgtaagtct tttttttttt tttgttgttg
     7321 ttggatgtac tggcacaatt atcatcagag taaatcagta actgttgtgc atttgcacag
     7381 aaagcataga aatgaactgt agttaaaaaa gctaacaatc ataaacaaag tagcatgtgc
     7441 atatactgta ctgaattttt tttaaataat aattgacata atcactggtt gtaatgtgta
     7501 aaaaatgagt ctgccttaac ccaacttcca ctctaaattg tgtgttgatg ctcatttccc
     7561 aaaagggctg aattacgata gaaaagataa gtcaacagtt tgttgtctct gcattgtttg
     7621 agttgactac atcttgagca aatgtagcta ttgtaaaatg gggatgagta tgtggtacag
     7681 agaaccatct cgtcaatgga gtggaaactg cattaacata gagagataga gagaacagct
     7741 gtgtctaggc atggggtata agattctgat ggtatgataa cctttgataa aaatatcaca
     7801 gtttgatggt attgtgattg ccactctaaa atatattatt ttttttaatg tcttagtaaa
     7861 aaacaaaaac tttaaacata atatatttta ttttgagaaa cattgaaaaa atattttgga
     7921 acagaaaaca tgtcaagctt aataatttca atgaatgatc gacttctgct gtcttcatta
     7981 gtttcaaaaa cagatttctt tacaatttaa aaagcatttt tgtatctctt ttctgctgaa
     8041 aatgctgttg tcctaaaaaa caaaaaaata aaaaataaat aaaaaatctt aaatatacct
     8101 taggaacggt atagcagaaa atattggtgg ttttaaaccc ttgacttttt caaaccgggg
     8161 tataccttga aaacggttat cgtcctatgc ctagctttgt caacaaccaa aaagcaactt
     8221 tattaacatc ttaaggcaag tttaaaggca acatctaaaa cagaatgaac ggttggagtt
     8281 tagtgaagtt tatttataaa ctaatttcga gagaatcacg tgcttatgac tgactacagc
     8341 tggtcccaca ttagcctatg tatgattcac caatcagaca attcctaact catcataggc
     8401 cgccagagtt cctttcattt cctcgaaaga gcggcacagc agcccagttg ttagcactgt
     8461 tgcctcaaag caagaacgtc actggtccaa gtctctaaca ggccagtcca tggttctgtg
     8521 tggagtttac atgttgtcct cgtgctcatg tggatatccc ctgggttctc cggttttctc
     8581 ctacagtcca aaaacttgcg acataggtga attgaacaaa accaaattag catagacaag
     8641 ctcctaatca gcagaatatc tctccgtagc aatctcttat tcgagatctg cgaagatacg
     8701 gccacgggtg ctcttgatcc tgtggctgat tttggactgt gctgctcgca gctgctgatg
     8761 aatcacatac ttcctccatt ttcttccact gattgactgt tataatttcc ctaatttcca
     8821 ggtcaaggtg ctgtgcattg tggtaataga tgtgacatga cgtcacttcc aaaggaccaa
     8881 tgaacatgtc tgaccaattt catataatgt gaaaacgatt ttcataggca gaataaataa
     8941 catttaaatt aaactgggca tcagcgcaat tcaattggtt tggtaatagc aagggaaaat
     9001 agaatgaagt gatctccaaa aaataagtac tttttgactg taaataaaat tgtaaggagt
     9061 aaaaagtact tttttttcta aaaaaatgta attaagtaaa agtaaaagta ttgattttta
     9121 attgtactca agtaaagtaa aaatccccaa aaataatact taagtacagt aatcaagtaa
     9181 aattactcaa gtactttaca cctctggttc ttgaccccct accttcagca agcccagcag
     9241 atccactagt tctagagcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt
     9301 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     9361 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     9421 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     9481 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     9541 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     9601 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     9661 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     9721 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     9781 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     9841 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     9901 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     9961 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
    10021 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    10081 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    10141 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
    10201 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    10261 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    10321 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    10381 aaggcatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
    10441 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
    10501 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    10561 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
    10621 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    10681 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    10741 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    10801 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    10861 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    10921 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    10981 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    11041 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    11101 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    11161 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    11221 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    11281 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    11341 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    11401 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    11461 catttccccg aaaagtg
//
