LOCUS       sequence-229433-       12256 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human SPRED1 and zebrafish mitfa specifically in zebrafish
            melanocytes .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12256)
  AUTHORS   Ablain J, Xu M, Rothschild H, Jordan RC, Mito JK, Daniels BH, Bell 
            CF, Joseph NM, Wu H, Bastian BC, Zon LI, Yeh I
  TITLE     Human tumor genomics and zebrafish modeling identify SPRED1 loss as 
            a driver of mucosal melanoma.
  JOURNAL   Science. 2018 Nov 1. pii: science.aau6509. doi: 
            10.1126/science.aau6509.
  PUBMED    30385465
REFERENCE   2  (bases 1 to 12256)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2018 Nov 1. pii: science.aau6509. doi: 10.1126/science.aau6509."
COMMENT     Sequence Label: MiniCoopR mitfa:SPRED1
FEATURES             Location/Qualifiers
     source          1..12256
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(514..525,526..688)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     1061..1077
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1097..1117
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2991..3015
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     3017..3033
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3043..3062
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3043..3061
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4478..4494)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(4531..4549)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    complement(4578..4602)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    4604..4738
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(4801..4821)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(4829..4845)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    4861..4995
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(9786..9973,9974..9985)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(10019..10035)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(10072..10090)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10111..10127)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10124..10146)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10135..10151
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10159..10165,10166..10183,10184..10189))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10204..10225
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10342..10359)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10513..11101)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10593..10612)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11272..12063,12064..12132))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11895..11914
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12133..12237)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 atgctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggccagatg ggccctcgag caggaaacag ctatgaccat
     1081 gattacgcca agctatcaac tttgtataga aaagttgtga gtgctaacaa ctgggctgct
     1141 gtgccgctct ttcgaggaaa tgaaaggaac tctggcggcc tatgatgagt taggaattgt
     1201 ctgattggtg aatcatacat aggctaatgt gggaccagct gtagtcagtc ataagcacgt
     1261 gattctctcg aaattagttt ataaataaac ttcactaaac tccaaccgtt cattctgttt
     1321 tagatgttgc ctttaaactt gccttaagat gttaataaag ttgctttttg gttgttgaca
     1381 aagctaggca taggacgata accgttttca aggtataccc cggtttgaaa aagtcaaggg
     1441 tttaaaacca ccaatatttt ctgctatacc gttcctaagg tatatttaag attttttatt
     1501 tattttttat ttttttgttt tttaggacaa cagcattttc agcagaaaag agatacaaaa
     1561 atgcttttta aattgtaaag aaatctgttt ttgaaactaa tgaagacagc agaagtcgat
     1621 cattcattga aattattaag cttgacatgt tttctgttcc aaaatatttt ttcaatgttt
     1681 ctcaaaataa aatatattat gtttaaagtt tttgtttttt actaagacat taaaaaaaat
     1741 aatatatttt agagtggcaa tcacaatacc atcaaactgt gatattttta tcaaaggtta
     1801 tcataccatc agaatcttat accccatgcc tagacacagc tgttctctct atctctctat
     1861 gttaatgcag tttccactcc attgacgaga tggttctctg taccacatac tcatccccat
     1921 tttacaatag ctacatttgc tcaagatgta gtcaactcaa acaatgcaga gacaacaaac
     1981 tgttgactta tcttttctat cgtaattcag cccttttggg aaatgagcat caacacacaa
     2041 tttagagtgg aagttgggtt aaggcagact cattttttac acattacaac cagtgattat
     2101 gtcaattatt atttaaaaaa aattcagtac agtatatgca catgctactt tgtttatgat
     2161 tgttagcttt tttaactaca gttcatttct atgctttctg tgcaaatgca caacagttac
     2221 tgatttactc tgatgataat tgtgccagta catccaacaa caacaaaaaa aaaaaaaaga
     2281 cttacaatca agccccaaag tgttcatacg tctggcaaat tctgacttaa agttactttt
     2341 attcaaccag ctagtttttt tttgactgta aatgacacgt aagataataa gatgatgtac
     2401 atgaggcatc attgtagaaa aaaaaatgaa tatgaatctg aatatgaact gtacgtctgt
     2461 gattttgttt gtgcgcactt acatacgtgt gcacaatatt tcaactctta agaaaaaaat
     2521 gcaattacca aaggcccatc agacaacaaa gttctctttc ctatcttttg ctcttcaaca
     2581 tttcagagat aactccatct taaactgtct tgtttttaga ccaacagtgc tagtgattgt
     2641 atgccggatg ctgagaacaa acaatgtttt atgcaggatg agtggaacta aagatgaaac
     2701 gttagttagc cgtaagccag tttcagttta gacactagta acccatcgtc tcaaagaggc
     2761 ttttgtcgaa tcggaccggt gacgtcaaat gaactttata aaggcataaa ttcatcgttt
     2821 gggtaaaaaa aacaatatga gaagagcaat cccctcccac ccccttaaat acattttgtg
     2881 tcttttcttt ggcattgaca tcttcagctg gccaagacga ctggtcagtt cttgcacatc
     2941 acctaaattc acaaagggaa tacttttttg aagctaacac atagttgaac gcaagtttgt
     3001 acaaaaaagc aggcttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag
     3061 ggcgaattgg gtaccgggcc ccccctcgag gtcgacgcca ccatgagcga ggagacggcg
     3121 acttctgaca acgataatag ttatgcacga gtgcgagctg tggtgatgac ccgagatgac
     3181 tcaagtggtg gatggttacc acttggaggg agtggactaa gcagcgtcac tgtcttcaaa
     3241 gtccctcatc aggaagagaa tggctgtgct gactttttta tccgtggaga gcgactcagg
     3301 gacaaaatgg tggttttgga atgtatgctt aaaaaagacc tcatttataa taaggtcact
     3361 ccaacatttc accactggaa gattgatgac aaaaagtttg gtcttacgtt tcaaagtcct
     3421 gctgatgcta gggcttttga tagaggtatc cgaagagcta tagaggatat ttctcaagga
     3481 tgccccgaat caaaaaatga agctgaaggg gcagatgact tacaagcaaa tgaagaggat
     3541 tcttccagtt ctctagtgaa ggatcacctt tttcagcaag agacagttgt taccagtgag
     3601 ccttatagaa gctcaaatat aagaccttct ccctttgaag atctgaatgc cagaagagtc
     3661 tacatgcaaa gccaagccaa tcagataaca tttggtcagc caggcttgga cattcagagc
     3721 agaagtatgg aatacgtaca gcggcaaata tccaaggaat gtggaagcct aaagtcccaa
     3781 aatagggtcc ctttgaaatc aatcagacat gtcagctttc aagatgagga tgagattgtc
     3841 agaataaacc ctcgagatat cttaatacgt cgctatgcag actacagaca tcctgacatg
     3901 tggaaaaatg acttggaaag agatgatgct gattccagta ttcagttttc taaaccagac
     3961 agtaaaaaat cagactatct gtactcttgt ggggatgaga ctaagttaag ttcacccaaa
     4021 gactctgtgg tatttaagac gcagccttcc tcattaaaaa ttaagaagtc aaaacgaaga
     4081 aaagaggatg gtgaacgttc tcgctgcgta tactgccagg aaaggtttaa tcatgaagaa
     4141 aatgtcaggg gaaaatgtca ggatgctcca gaccctatta aaagatgcat atatcaagtt
     4201 agttgcatgc tctgtgcaga gagcatgttg tatcattgta tgtcagactc agagggagat
     4261 ttttctgatc cctgttcgtg tgacactagc gacgacaagt tctgcttgcg atggttagcc
     4321 ctggtagctt tgtctttcat tgtaccatgt atgtgctgct acgtcccttt gagaatgtgc
     4381 catcgctgtg gtgaggcatg tggttgctgt ggtgggaaac ataaagctgc tggattgtaa
     4441 atcgataagc ttgatatcga attcctgcag cccgggggat ccactagttc tagagcggcc
     4501 gccaccgcgg tggagctcca gcttttgttc cctttagtga gggttaattg cgcgcttggc
     4561 gtaatcatgg tcatagcacc cagctttctt gtacaaagtg ggggatccag acatgataag
     4621 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     4681 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     4741 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggttttttc
     4801 caactttatt atacatagtt gataattcac tggccgtcgt tttacggtac catcgatgat
     4861 gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     4921 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     4981 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     5041 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat
     5101 cctctagatc agatctcaga ttgtatccaa aatatcattt tttgtgtttt aaagatgaac
     5161 aaaggtctca gggaattgga acgacatgag gatgattaat taataaaaaa aaattcattt
     5221 ttgggtgaac tatccctaaa atcaaagcaa tttgggcatt ttttttctaa ttattatgga
     5281 gaacttgcat cacagctctt ccactagaaa ataaaacaaa tttcagaaat actgcagaaa
     5341 tacttttgga cttaatgtta aataacaatg taaaaagttt ttccagggca tcgtggtggt
     5401 gcagtgggta gcattgtcgc cttacagcaa gaaggtcgct ggttcgagac ttggttgggt
     5461 catttctgtg tggagtttgc atgttctccc catgttcgtg tgggtttcct ccgggtgctc
     5521 cagtttcacc tacagcccaa agacatgcgc tataggtgaa ttgggtaagc taaattgtct
     5581 aaagtgaatg gtactgtggt gggtgtttcc cagtgatggg ttgcagctgc aagggcatcc
     5641 gctggatttg tttgttggca taagttggca gttcattcaa ctgtggcaac ccctgattaa
     5701 taaaggggct aagccaaaaa gaaaatgaat gactgaatga aagtgaatcc agttgtacat
     5761 gcctagacat gtcctccacc cttgcaagat gtacacccca agaaatggag ctgcatttga
     5821 ggacaacaat aagccaagcc tgcaaataaa agacacctca acagttttta ttcaagctac
     5881 tctttatatc atccgcgaga atccttcaaa aatcaactaa ttgtttacac gatttcattc
     5941 ttgactagac tctgacatga taaatgaaaa aatatataca caagcacact aaatattctg
     6001 aaatacaaga ttaatacttt acgaaattgc gctcttcatg atctggatgt aacactggta
     6061 cttacaagac acacacacac acaaatctaa ttgtctaact ttggttcatg aaatttagtt
     6121 ggcattgcta acagccattg tcatgttcgt ccatactgct gctgccgcta ctgttggaag
     6181 gtactgggga tccggaggac aacagcgggt cgctgctgcc caccggagac agggtgttgt
     6241 ccataagcat gtcctccagc ttggtggagg ccttgtttgg gctgtcgtag acccccggct
     6301 cgccgtcctc cgtgggactg tcattgtagc tgatggtgcc gttgttgagg tccagagtgg
     6361 taggacggga catgtcagga ctgggaaggt gtgggtacaa attggatgtg cagtccccca
     6421 tcccaggctc ttgtttgatt gctcgagcca ctaactcagc ggaataaagg ctggaagaag
     6481 ctacaacggt gagtccatga gcacgggctt gcatttcaag ttcctgaata cggagcagga
     6541 gatgtctgtt tgcgtgttct agtctcttct gtctgttttc cagctctttt gctttctgct
     6601 gctctttctg caatttccta atgtaatcca ctgatgcttt caggatggtg cctttattcc
     6661 acctcatgtc tggatcattt gacttgggaa ttaaagtccc cagctcctta attctgtcat
     6721 tgatattaaa ccgccttctt cgttcaatga ggttgtggtt gtccttcttt tgtctctctt
     6781 ttgccagggc tctgacttct gcttctactg gaaagccatc aggtctttga taagagtcaa
     6841 acttgccaca tggtccagct ttgtccatta tgtgcatcat gcccggagat ggagtaacgg
     6901 ataattccct tttgacggcc ggcaggcttg aggggcagga gttactgatg gaaactccag
     6961 ctggaggaag agcatgattg ctgtacatgt ccagcaggtt tgctgaaact ggaatcgtgt
     7021 ttgtcatttg aagtcctgca tccatgaacc caagaatgtc atcactgtag cttgattcca
     7081 aacttataat gtcttcaatt acatcgtcca tctctttctc acagttgagg gtgagaaggg
     7141 ccatagggct gttgggagcg ctggctccgg gtcccggggt catgccgtgc tcggcgggct
     7201 ggctggggcc gggccctgtg ctggcctgtg ggctcagctt ggctcccagt gcactggaca
     7261 ggtagtgctt cacctgctgc ctctggctct gctggatgtg gtacttcgag ggggtttcca
     7321 ggtgggtctg aacctggtag tgactgtact cgagcatctc caacatgttc aactatgtgt
     7381 tagcttcaaa aaagtattcc ctttgtgaat ttaggtgatg tgcaagaact gaccagtcgt
     7441 cttggccagc tgaagatgtc aatgccaaag aaaagacaca aaatgtattt aagggggtgg
     7501 gaggggattg ctcttctcat attgtttttt ttacccaaac gatgaattta tgcctttata
     7561 aagttcattt gacgtcaccg gtccgattcg acaaaagcct ctttgagacg atgggttact
     7621 agtgtctaaa ctgaaactgg cttacggcta actaacgttt catctttagt tccactcatc
     7681 ctgcataaaa cattgtttgt tctcagcatc cggcatacaa tcactagcac tgttggtcta
     7741 aaaacaagac agtttaagat ggagttatct ctgaaatgtt gaagagcaaa agataggaaa
     7801 gagaactttg ttgtctgatg ggcctttggt aattgcattt ttttcttaag agttgaaata
     7861 ttgtgcacac gtatgtaagt gcgcacaaac aaaatcacag acgtacagtt catattcaga
     7921 ttcatattca tttttttttc tacaatgatg cctcatgtac atcatcttat tatcttacgt
     7981 gtcatttaca gtcaaaaaaa aactagctgg ttgaataaaa gtaactttaa gtcagaattt
     8041 gccagacgta tgaacacttt ggggcttgat tgtaagtctt tttttttttt ttgttgttgt
     8101 tggatgtact ggcacaatta tcatcagagt aaatcagtaa ctgttgtgca tttgcacaga
     8161 aagcatagaa atgaactgta gttaaaaaag ctaacaatca taaacaaagt agcatgtgca
     8221 tatactgtac tgaatttttt ttaaataata attgacataa tcactggttg taatgtgtaa
     8281 aaaatgagtc tgccttaacc caacttccac tctaaattgt gtgttgatgc tcatttccca
     8341 aaagggctga attacgatag aaaagataag tcaacagttt gttgtctctg cattgtttga
     8401 gttgactaca tcttgagcaa atgtagctat tgtaaaatgg ggatgagtat gtggtacaga
     8461 gaaccatctc gtcaatggag tggaaactgc attaacatag agagatagag agaacagctg
     8521 tgtctaggca tggggtataa gattctgatg gtatgataac ctttgataaa aatatcacag
     8581 tttgatggta ttgtgattgc cactctaaaa tatattattt tttttaatgt cttagtaaaa
     8641 aacaaaaact ttaaacataa tatattttat tttgagaaac attgaaaaaa tattttggaa
     8701 cagaaaacat gtcaagctta ataatttcaa tgaatgatcg acttctgctg tcttcattag
     8761 tttcaaaaac agatttcttt acaatttaaa aagcattttt gtatctcttt tctgctgaaa
     8821 atgctgttgt cctaaaaaac aaaaaaataa aaaataaata aaaaatctta aatatacctt
     8881 aggaacggta tagcagaaaa tattggtggt tttaaaccct tgactttttc aaaccggggt
     8941 ataccttgaa aacggttatc gtcctatgcc tagctttgtc aacaaccaaa aagcaacttt
     9001 attaacatct taaggcaagt ttaaaggcaa catctaaaac agaatgaacg gttggagttt
     9061 agtgaagttt atttataaac taatttcgag agaatcacgt gcttatgact gactacagct
     9121 ggtcccacat tagcctatgt atgattcacc aatcagacaa ttcctaactc atcataggcc
     9181 gccagagttc ctttcatttc ctcgaaagag cggcacagca gcccagttgt tagcactgtt
     9241 gcctcaaagc aagaacgtca ctggtccaag tctctaacag gccagtccat ggttctgtgt
     9301 ggagtttaca tgttgtcctc gtgctcatgt ggatatcccc tgggttctcc ggttttctcc
     9361 tacagtccaa aaacttgcga cataggtgaa ttgaacaaaa ccaaattagc atagacaagc
     9421 tcctaatcag cagaatatct ctccgtagca atctcttatt cgagatctgc gaagatacgg
     9481 ccacgggtgc tcttgatcct gtggctgatt ttggactgtg ctgctcgcag ctgctgatga
     9541 atcacatact tcctccattt tcttccactg attgactgtt ataatttccc taatttccag
     9601 gtcaaggtgc tgtgcattgt ggtaatagat gtgacatgac gtcacttcca aaggaccaat
     9661 gaacatgtct gaccaatttc atataatgtg aaaacgattt tcataggcag aataaataac
     9721 atttaaatta aactgggcat cagcgcaatt caattggttt ggtaatagca agggaaaata
     9781 gaatgaagtg atctccaaaa aataagtact ttttgactgt aaataaaatt gtaaggagta
     9841 aaaagtactt ttttttctaa aaaaatgtaa ttaagtaaaa gtaaaagtat tgatttttaa
     9901 ttgtactcaa gtaaagtaaa aatccccaaa aataatactt aagtacagta atcaagtaaa
     9961 attactcaag tactttacac ctctggttct tgacccccta ccttcagcaa gcccagcaga
    10021 tccactagtt ctagagcggc cgccaccgcg gtggagctcc agcttttgtt ccctttagtg
    10081 agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
    10141 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
    10201 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
    10261 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
    10321 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
    10381 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
    10441 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
    10501 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
    10561 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
    10621 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
    10681 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
    10741 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
    10801 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
    10861 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    10921 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
    10981 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
    11041 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    11101 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    11161 aggcattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
    11221 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
    11281 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    11341 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
    11401 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
    11461 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    11521 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    11581 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    11641 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    11701 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    11761 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    11821 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    11881 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    11941 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    12001 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    12061 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    12121 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    12181 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    12241 atttccccga aaagtg
//
