LOCUS       sequence-229435-        4702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  12x Widom 601 nucleosome sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Swygert SG, Senapati S, Bolukbasi MF, Wolfe SA, Lindsay S, Peterson 
            CL
  TITLE     SIR proteins create compact heterochromatin fibers.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Dec 4;115(49):12447-12452. doi: 
            10.1073/pnas.1810647115. Epub 2018 Nov 19.
  PUBMED    30455303
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1810647115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-12-4- 4"; volume: "115"; issue: "49"; pages: 
            "12447-12452"
COMMENT     Sequence Label: pBS_12x-601_MA3
FEATURES             Location/Qualifiers
     source          1..4702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..74
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     66..83
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     67..83
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2698..3489
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3582..4170
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4071..4090
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4324..4341
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4500..4527)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4619..4700)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4659..4702
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac
       61 gacgttgtaa aacgacggcc agtcttaagc gtctccccct gaacctgacc ccggaccaag
      121 tggtggctat tctagataac tgctcttagg cacaggatgt atatatctga cacgtgcctg
      181 gagactaggg agtaatcccc ttggcggtta aaacgcgggg gacagcgcgt acgtgcgttt
      241 aagcggtgct agagctgtct acgaccaatt gagcggcctc ggcaccggga ttctccaggt
      301 actggagaat tccagttcca tcagaagcac aggatgtata tatctgacac gtgcctggag
      361 actagggagt aatccccttg gcggttaaaa cgcgggggac agcgcgtacg tgcgtttaag
      421 cggtgctaga gctgtctacg accaattgag cggcctcggc accgggattc tccaggtact
      481 ggagaattcg gacgacagtg tgagcacagg atgtatatat ctgacacgtg cctggagact
      541 agggagtaat ccccttggcg gttaaaacgc gggggacagc gcgtacgtgc gtttaagcgg
      601 tgctagagct gtctacgacc aattgagcgg cctcggcacc gggattctcc aggtactgga
      661 gaattcccag ggatccttga gcacaggatg tatatatctg acacgtgcct ggagactagg
      721 gagtaatccc cttggcggtt aaaacgcggg ggacagcgcg tacgtgcgtt taagcggtgc
      781 tagagctgtc tacgaccaat tgagcggcct cggcaccggg attctccagg tactggagaa
      841 ttctgtttac accgacggca caggatgtat atatctgaca cgtgcctgga gactagggag
      901 taatcccctt ggcggttaaa acgcggggga cagcgcgtac gtgcgtttaa gcggtgctag
      961 agctgtctac gaccaattga gcggcctcgg caccgggatt ctccaggtac tggagaattc
     1021 atgctaacga gatcgcacag gatgtatata tctgacacgt gcctggagac tagggagtaa
     1081 tccccttggc ggttaaaacg cggggggtcg accgtacgtg cgtttaagcg gtgctagagc
     1141 tgtctacgac caattgagcg gcctcggcac cgggattctc caggtactgg agaattccgg
     1201 tccgtttacc agcacaggat gtatatatct gacacgtgcc tggagactag ggagtaatcc
     1261 ccttggcggt taaaacgcgg gggacagcgc gtacgtgcgt ttaagcggtg ctagagctgt
     1321 ctacgaccaa ttgagcggcc tcggcaccgg gattctccag gttgcgtggg cgggaaagaa
     1381 ttcgctagca caggatgtat atatctgaca cgtgcctgga gactagggag taatcccctt
     1441 ggcggttaaa acgcggggga cagcgcgtac gtgcgtttaa gcggtgctag agctgtctac
     1501 gaccaattga gcggcctcgg caccgggatt ctccaggtac tggagaattc tacgcatata
     1561 ctatgcacag gatgtatata tctgacacgt gcctggagac tagggagtaa tccccttggc
     1621 ggttaaaacg cgggggacag cgcgtacgtg cgtttaagcg gtgctagagc tgtctacgac
     1681 caattgagcg gcctcggcac cgggattctc caggtactgg agaattcgct caagcttgat
     1741 cgcacaggat gtatatatct gacacgtgcc tggagactag ggagtaatcc ccttggcggt
     1801 taaaacgcgg gggacagcgc gtacgtgcgt ttaagcggtg ctagagctgt ctacgaccaa
     1861 ttgagcggcc tcggcaccgg gattctccag gtactggaga attcagccca cgcagacggc
     1921 acaggatgta tatatctgac acgtgcctgg agactaggga gtaatcccct tggcggttaa
     1981 aacgcggggg acagcgcgta cgtgcgttta agcggtgcta gagctgtcta cgaccaattg
     2041 agcggcctcg gcaccgggat tctccaggta ctggagaatt cgcgtgtaat acactgcaca
     2101 ggatgtatat atctgacacg tgcctggaga ctagggagta atccccttgg cggttaaaac
     2161 gcgggggaca gcgcgtacgt gcgtttaagc ggtgctagag ctgtctacga ccaattgagc
     2221 ggcctcggca ccgggattct ccaggtagaa ttcactagtg cggcatgcgg cggcaagcaa
     2281 gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg ccgagacgtc
     2341 tagaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc
     2401 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
     2461 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
     2521 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
     2581 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
     2641 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg
     2701 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
     2761 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
     2821 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
     2881 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
     2941 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
     3001 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
     3061 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
     3121 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
     3181 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
     3241 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
     3301 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     3361 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     3421 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     3481 ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc
     3541 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     3601 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     3661 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     3721 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     3781 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     3841 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     3901 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     3961 ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     4021 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     4081 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     4141 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     4201 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     4261 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     4321 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     4381 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     4441 agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa
     4501 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     4561 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     4621 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     4681 ccgactgagc ctttcgtttt at
//
