LOCUS       sequence-229453-        8110 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Please note this plasmid is discontinued. If you need this plasmid 
            for experiments, please directly contact Robert Campbell at 
            University of Alberta..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8110)
  AUTHORS   Belal AS, Sell BR, Hoi H, Davidson MW, Campbell RE
  TITLE     Optimization of a genetically encoded biosensor for cyclin B1-cyclin
            dependent kinase 1.
  JOURNAL   Mol Biosyst. 2014 Feb;10(2):191-5. doi: 10.1039/c3mb70402e.
  PUBMED    24281384
REFERENCE   2  (bases 1 to 8110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-02"; volume: "10"; issue: "2"; pages: 
            "191-5"
COMMENT     Sequence Label: cyclin B1-CDK1 FRET biosensor
FEATURES             Location/Qualifiers
     source          1..8110
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(46..65)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        237..616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        617..820
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     771..791
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     865..884
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        865..883
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      900..909
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             918..1625
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             join(2184..2186,2187..2189,2190..2900)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2229..2250)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2490..2509)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2837..2858
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2912..3625
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLE
                     FLTAAGITEGMNELYK"
     promoter        complement(3665..3683)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3666..3683)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3703..3720)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3709..3933
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3979..4407
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4066..4085)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4276..4297
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4416..4436)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4421..4750
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4601..4736
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4663..4682
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4817..5611
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4871..4890)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5481..5500
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5785..5906
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5822..5841)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5876..5895
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5955..5971)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5955..5971)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5968..5990)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5979..5995
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6003..6009,6010..6027,6028..6033))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6048..6069
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6186..6203)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6357..6945)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6437..6456)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7116..7907,7908..7976))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7739..7758
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7977..8081)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc
      841 cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc aagctagccg
      901 ccaccatggc ctcgagcatg gtgagcaagg gcgaggagac cacaatgggc gtaatcaagc
      961 ccgacatgaa gatcaagctg aagatggagg gcaacgtgaa tggccacgcc ttcgtgatcg
     1021 agggcgaggg cgagggcaag ccctacgacg gcaccaacac catcaacctg gaggtgaagg
     1081 agggagcccc cctgcccttc tcctacgaca ttctgaccac cgcgttcgcc tacggcaaca
     1141 gggccttcac caagtacccc gacgacatcc ccaactactt caagcagtcc ttccccgagg
     1201 gctactcttg ggagcgcacc atgaccttcg aggacaaggg catcgtgaag gtgaagtccg
     1261 acatctccat ggaggaggac tccttcatct acgagataca cctcaagggc gagaacttcc
     1321 cccccaacgg ccccgtgatg cagaagaaga ccaccggctg ggacgcctcc accgagagga
     1381 tgtacgtgcg cgacggcgtg ctgaagggcg acgtcaagca caagctgctg ctggagggcg
     1441 gcggccacca ccgcgttgac ttcaagacca tctacagggc caagaaggcg gtgaagctgc
     1501 ccgactatca ctttgtggac caccgcatcg agatcctgaa ccacgacaag gactacaaca
     1561 aggtgaccgt ttacgagagc gccgtggccc gcaactccac cgacggcatg gacgagctgt
     1621 acaagcctag gttaacttta aaaccattgc ctgacagcat tattcaagaa agcctggaga
     1681 ttcagcaagg tgtgaatcca tttttcattg gtagatccga ggattgcaat tgtaaaattg
     1741 aagacaatag gttgtcacga gttcattgct tcattttcaa aaagaggcat gctgtaggca
     1801 aaagcatgta tgaatctccg gcacaaggtt tagatgatat ttggtattgc cacaccggaa
     1861 ctaacgtgag ctatttaaat aataaccgca tgatacaggg tacgaaattc cttttacaag
     1921 acggagatga aatcaagatc atttgggata aaaacaataa atttgtcatt ggctttaaag
     1981 tggaaattaa cgatactaca ggtctgttta acgagggatt aggtatgtta caagaacaaa
     2041 gagtagtact taagcaaaca gccgaagaaa aagatttggt gaaaaagtta gtcgacggcg
     2101 gcagcggcgg cggcggcagc ggcggcaccc ctgagcctat tttggttgat acttcctctc
     2161 caagcccaat ggaaacaggt accatggtga gcaagggcga ggagctgttc accggggtgg
     2221 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     2281 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     2341 agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg atgtgcttcg
     2401 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     2461 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     2521 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     2581 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     2641 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     2701 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     2761 ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca
     2821 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     2881 gcatggacga gctgtacaag taaaagcttg gatgtctaaa ggtgaagaat tattcactgg
     2941 tgttgtccca attttggttg aattagatgg tgatgttaat ggtcacaaat tttctgtctc
     3001 cggtgaaggt gaaggtgatg ctacgtacgg taaattgacc ttaaaattac tctgtactac
     3061 tggtaaattg ccagttccat ggccaacctt agtcactact ttaggttatg gtgttcaatg
     3121 ttttgctaga tacccagatc atatgaaaca acatgacttt ttcaagtctg ccatgccaga
     3181 aggttatgtt caagaaagaa ctattttttt caaagatgac ggtaactaca agaccagagc
     3241 tgaagtcaag tttgaaggtg ataccttagt taatagaatc gaattaaaag gtattgattt
     3301 taaagaagat ggtaacattt taggtcacaa attggaatac aactataact ctcacaatgt
     3361 ttacatcact gctgacaaac aaaagaatgg tatcaaagct aacttcaaaa ttagacacaa
     3421 cattgaagat ggtggtgttc aattagctga ccattatcaa caaaatactc caattggtga
     3481 tggtccagtc ttgttaccag acaaccatta cttatcctat caatctgcct tattcaaaga
     3541 tccaaacgaa aagagagacc acatggtctt gttagaattt ttgactgctg ctggtattac
     3601 cgagggtatg aatgaattgt acaaataaga attcatgggc aggccggcct aaactgggcc
     3661 ctattctata gtgtcaccta aatgctagag ctcgctgatc agcctcgact gtgccttcta
     3721 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     3781 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     3841 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     3901 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     3961 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     4021 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     4081 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     4141 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     4201 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     4261 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     4321 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     4381 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     4441 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     4501 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     4561 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     4621 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     4681 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     4741 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg
     4801 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     4861 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     4921 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     4981 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     5041 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     5101 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     5161 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     5221 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     5281 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     5341 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     5401 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     5461 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     5521 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     5581 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     5641 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     5701 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     5761 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     5821 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     5881 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     5941 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     6001 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     6061 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     6121 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     6181 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     6241 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     6301 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     6361 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     6421 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     6481 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     6541 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     6601 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     6661 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     6721 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     6781 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     6841 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     6901 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     6961 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     7021 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     7081 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     7141 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     7201 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     7261 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     7321 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     7381 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     7441 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     7501 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     7561 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     7621 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     7681 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     7741 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     7801 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     7861 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     7921 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     7981 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     8041 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     8101 ccacctgacg
//
